Gold nanoparticle-based multivalent carbohydrate probes: selective photoaffinity labeling of carbohydrate-binding proteins

Sakurai, Kaori; Hatai, Yuki; Okada, Ayumi

doi:10.1039/c5sc03275j

Cited by 30 publications

(36 citation statements)

References 44 publications

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“…In this regard, we judiciously combined a BA with the photoaffinity labelling (PAL) reagent trifluoromethylphenyl diazirine (Diaz) for irreversible crosslinking of the constant Fc region of mAbs to planar glass surfaces without affecting the antigen binding affinity, which showed resistance to fouling from biological fluids 37. Recently, PAL reagent-functionalized glyco-gold NPs were introduced and described for the enrichment of photocrosslinked lectins 38. To fully facilitate the implementation of covalent Ab conjugation in the development of biomolecular assays, further studies are essential to expand the scope of materials that can be surface engineered by UV light activation.…”

Section: Introductionmentioning

confidence: 99%

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

et al. 2019

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“…In another example, difunctionalized GNPs were assembled from a multivalent carbohydrate and a photoreactive group for affinity extraction of carbohydrate‐binding proteins . The enriched protein was analyzed by MALDI‐TOF MS analysis and gel based fluorescence imaging after release using 2‐mercaptoethanol.…”

Section: Gnps In Sample Preparationmentioning

confidence: 99%

Functionalized gold nanoparticles for sample preparation: A review

Liu

Lämmerhofer

2019

Electrophoresis

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Sample preparation is a crucial step for the reliable and accurate analysis of both small molecule and biopolymers which often involves processes such as isolation, pre‐concentration, removal of interferences (purification), and pre‐processing (e.g., enzymatic digestion) of targets from a complex matrix. Gold nanoparticle (GNP)‐assisted sample preparation and pre‐concentration has been extensively applied in many analytical procedures in recent years due to the favorable and unique properties of GNPs such as size‐controlled synthesis, large surface‐to‐volume ratio, surface inertness, straightforward surface modification, easy separation requiring minimal manipulation of samples. This review article primarily focuses on applications of GNPs in sample preparation, in particular for bioaffinity capture and biocatalysis. In addition, their most common synthesis, surface modification and characterization methods are briefly summarized. Proper surface modification for GNPs designed in accordance to their target application directly influence their functionalities, e.g., extraction efficiencies, and catalytic efficiencies. Characterization of GNPs after synthesis and modification is worthwhile for monitoring and controlling the fabrication process to ensure proper quality and functionality. Parameters such as morphology, colloidal stability, and physical/chemical properties can be assessed by methods such as surface plasmon resonance, dynamic light scattering, ζ‐potential determinations, transmission electron microscopy, Taylor dispersion analysis, and resonant mass measurement, among others. The accurate determination of the surface coverage appears to be also mandatory for the quality control of functionality of the nanoparticles. Some promising applications of (functionalized) GNPs for bioanalysis and sample preparation are described herein.

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“…13b ). In addition to the probes, Kaori Sakurai et al [ 59 ] developed gold nanoparticle-based multivalent carbohydrate probes for selective PAL of carbohydrate-binding proteins. The probes were assembled using AuNPs as scaffolds, a carbohydrate ligand and a photoreactive group in a modular fashion (Fig.…”

Section: Benzophenone-based Probes To Study Ppimentioning

confidence: 99%

Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

et al. 2016

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Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

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Gold nanoparticle-based multivalent carbohydrate probes: selective photoaffinity labeling of carbohydrate-binding proteins

Abstract: Multivalent carbohydrate photoaffinity probes were developed based on gold nanoparticles (AuNPs) to provide a streamlined approach toward identification of carbohydrate-binding proteins.

Cited by 30 publications

References 44 publications

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies

Functionalized gold nanoparticles for sample preparation: A review

Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

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