Crystal structure of PilF: Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

Kim, Kyunggon; Oh, Jongkil; Han, Dohyun; Kim, Eunice EunKyeong; Lee, Byung‐Il; Kim, Youngsoo

doi:10.1016/j.bbrc.2005.12.108

Cited by 54 publications

(56 citation statements)

References 44 publications

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“…A total of 25 components are involved in the type IV pilus biogenesis (Kim et al, 2006). In the present small-scale investigation we observed ten mutations affecting five genes.…”

Section: Cross-resistance and Reversibility Of Mutantsmentioning

confidence: 64%

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Latino

2016

Microbiology

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Coevolution between bacteriophages (phages) and their prey is the result of mutualistic interactions. Here, we show that pseudolysogeny is a frequent outcome of infection by virulent phages of Pseudomonas aeruginosa and that selection of resistant bacterial mutants is favoured by continuous production of phages. We investigated the frequency and characteristics of P. aeruginosa strain PAO1 variants resisting infection by different combinations of virulent phages belonging to four genera. The frequency of resistant bacteria was 10 25 for single phage infection and 10 26 for infections with combinations of two or four phages. The genome of 27 variants was sequenced and the comparison with the genome of the parental PAO1 strain allowed the identification of point mutations or small indels. Four additional variants were characterized by a candidate gene approach. In total, 27 independent mutations were observed affecting 14 genes and a regulatory region. The mutations affected genes involved in biosynthesis of type IV pilus, alginate, LPS and O-antigen. Half of the variants possessed changes in homopolymer tracts responsible for frameshift mutations and these phase variation mutants were shown to be unstable. Eleven double mutants were detected. The presence of free phage DNA was observed in association with exclusion of superinfection in half of the variants and no chromosomal mutation could be found in three of them. Upon further growth of these pseudolysogens, some variants with new chromosomal mutations were recovered, presumably due to continuous evolutionary pressure.

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“…A total of 25 components are involved in the type IV pilus biogenesis (Kim et al, 2006). In the present small-scale investigation we observed ten mutations affecting five genes.…”

Section: Cross-resistance and Reversibility Of Mutantsmentioning

confidence: 64%

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Latino

2016

Microbiology

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“…G82R frameshifts frameshift W75A with such a view, the very C terminus of the P. aeruginosa PilZ protein has recently been proposed to interact with the PilF protein required for type 4 pilus assembly (37). To complement our picture of the c-di-GMP circuitry, future studies will have to focus on interaction partners of DgrA and related PilZ domain proteins.…”

Section: Discussionmentioning

confidence: 99%

DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus

Christen

Allan

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

186

218

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Bacteria are able to switch between two mutually exclusive lifestyles, motile single cells and sedentary multicellular communities that colonize surfaces. These behavioral changes contribute to an increased fitness in structured environments and are controlled by the ubiquitous bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP). In response to changing environments, fluctuating levels of c-di-GMP inversely regulate cell motility and cell surface adhesins. Although the synthesis and breakdown of c-di-GMP has been studied in detail, little is known about the downstream effector mechanisms. Using affinity chromatography, we have isolated several c-di-GMP-binding proteins from Caulobacter crescentus . One of these proteins, DgrA, is a PilZ homolog involved in mediating c-di-GMP-dependent control of C. crescentus cell motility. Biochemical and structural analysis of DgrA and homologs from C. crescentus , Salmonella typhimurium , and Pseudomonas aeruginosa demonstrated that this protein family represents a class of specific diguanylate receptors and suggested a general mechanism for c-di-GMP binding and signal transduction. Increased concentrations of c-di-GMP or DgrA blocked motility in C. crescentus by interfering with motor function rather than flagellar assembly. We present preliminary evidence implicating the flagellar motor protein FliL in DgrA-dependent cell motility control.

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“…However, with the exception of the bacterial PilF protein from Pseudomonas aeruginosa (rmsd of 4.1 Å over 200 Ca atoms; 8% sequence identity) (Kim et al 2006), these proteins display high rmsd values compared with the entire crystallized Nro1 fragment (rmsd of 7-9 Å for structural alignment of 200-230 residues). Since the structure of Nro1 can be divided into two domains (Nro1-N and Nro1-C), we have searched the structure database for the subdomains separately.…”

Section: Comparison With Other Tpr Proteinsmentioning

confidence: 98%

Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O₂-mediated gene control in S. pombe

Rispal¹,

Henri²,

Tilbeurgh³

et al. 2011

RNA

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In Saccharomyces cerevisiae, the putative 2-OG-Fe(II) dioxygenase Tpa1 and its partner Ett1 have been shown to impact mRNA decay and translation. Hence, inactivation of these factors was shown to influence stop codon read-though. In addition, Tpa1 represses, by an unknown mechanism, genes regulated by Hap1, a transcription factor involved in the response to levels of heme and O 2 . The Schizosaccharomyces pombe orthologs of Tpa1 and Ett1, Ofd1, and its partner Nro1, respectively, have been shown to regulate the stability of the Sre1 transcription factor in response to oxygen levels. To gain insight into the function of Nro1/Ett1, we have solved the crystal structure of the S. pombe Nro1 protein deleted of its 54 N-terminal residues. Nro1 unexpectedly adopts a Tetratrico Peptide Repeat (TPR) fold, a motif often responsible for protein or peptide binding. Two ligands, a sulfate ion and an unknown molecule, interact with a cluster of highly conserved amino acids on the protein surface. Mutation of these residues demonstrates that these ligand binding sites are essential for Ett1 function in S. cerevisiae, as investigated by assaying for efficient translation termination.

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Crystal structure of PilF: Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

Cited by 54 publications

References 44 publications

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus

Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O₂-mediated gene control in S. pombe

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Crystal structure of PilF: Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

Cited by 54 publications

References 44 publications

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

Pseudolysogeny and sequential mutations build multiresistance to virulent bacteriophages in Pseudomonas aeruginosa

DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus

Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O2-mediated gene control in S. pombe

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Structural and functional analysis of Nro1/Ett1: a protein involved in translation termination in S. cerevisiae and in O₂-mediated gene control in S. pombe