Crystal structure of an acetyl-CoA acetyltransferase from PHB producing bacterium Bacillus cereus ATCC 14579

Hong, Jung Hwa; Park, Woojin; Seo, Hogyun; Kim, Il-Kwon; Kim, Kyungjin

doi:10.1016/j.bbrc.2020.09.048

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…The present study suggested that p -nitrophenyl acyl chains bind in Channel A prior to hydrolytic cleavage, and previous data support the OleA-catalyzed Claisen reaction following a ping-pong mechanism like other thiolases [25] , [18] , [19] , [20] , [21] , [22] . Via that mechanism, acyl transfer from the p -nitrophenyl ester to C143 is followed by leaving of the first product, p -nitrophenol ( Fig.…”

Section: Resultssupporting

confidence: 86%

“…1 A). Thiolase enzymes are involved in many different pathways in the metabolism of fatty acids, polyhydroxybutyrate storage, and natural product biosynthesis [18] , [19] , [20] , [21] , [22] . OleA homologs that produce natural products include NltAB, a heterodimer biosynthesized by a Nocardia species that produces the β-lactone nocardiolactone [23] ( Fig.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA

Smith

Tassoulas²,

Biernath³

et al. 2021

Computational and Structural Biotechnology Journal

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA

Smith

Tassoulas²,

Biernath³

et al. 2021

Computational and Structural Biotechnology Journal

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show abstract

“…It is also necessary to develop open culture of Bacillus. Additionally, Bacillus PHA surface binding protein including PhaR, PhaP, PhaQ, PhaC have different composition from Gram-negative bacteria, mainly in amino acid composition and protein structure (Hong et al 2020). Thus far, there is no research on the application of PHA surface binding protein from Bacillus.…”

Section: Introductionmentioning

confidence: 99%

Alkali-Tolerant Bacillus Producing PHA and Application of its PHA Surface Binding Protein as Surfactant

Fan

Jiang

et al. 2023

Preprint

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Polyhydroxyalkanoates (PHA) produced by Bacillus bacteria have potential advantages in medical applications, due to its cell membrane do not contain outer membrane lipopolysaccharide (LPS). However, the PHA accumulation level is far lower than that of Gram-negative bacteria. In this study, an alkali-tolerant Bacillus cereus HBL-AI was screened using Nile Red and Sudan Black B in a Low-Salt LB solid medium at pH 8.0. This strain displayed high PHA accumulation capacity in fed‑batch fermentation with glucose as the carbon source in a 5 L unsterilized fermentor. The highest PHA accumulation reached 12.8 g/L and the extracted product was finally determined as (Poly-β-3-hydroxybutyrate, PHB). It has the potential to be used for large-scale open and continuous fermentation. Furthermore, PHA membrane binding protein (PhaP), PHA regulatory protein (PhaQ and PhaR) derived from HBL-AI were heterologously expressed inE. coli and purified to investigate their application as biosurfactants. The results showed that the emulsification ability and stability of three amphiphilic proteins were higher than those of widely used chemical surfactants. The protein concentration of PhaQ used to form a stable emulsion layer in vegetable oil was the lowest (50 μg/mL), which greatly reduced the amount of protein used in emulsification. This clearly demonstrated that HBL-AI has great potential in the production of PHA without endotoxin, and its PHA binding protein can be well applied as an environmentally friendly biosurfactants.

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Harnessing economical biopolymer extrusion: the Bacillus clade as endotoxin-free platforms for next-generation bioprocesses

Kumar,

Li,

Mishra

et al. 2024

Rev Environ Sci Biotechnol

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Crystal structure of an acetyl-CoA acetyltransferase from PHB producing bacterium Bacillus cereus ATCC 14579

Cited by 9 publications

References 26 publications

p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA

p-Nitrophenyl esters provide new insights and applications for the thiolase enzyme OleA

Alkali-Tolerant Bacillus Producing PHA and Application of its PHA Surface Binding Protein as Surfactant

Harnessing economical biopolymer extrusion: the Bacillus clade as endotoxin-free platforms for next-generation bioprocesses

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