Crystal seeding by salivary calculus and its inhibition in vitro

Turesky, Samuel; Glickman, Irving; Krasnoff, Miriam

doi:10.1016/0003-9969(65)90027-0

Cited by 12 publications

(4 citation statements)

References 7 publications

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“…Metal salts can retard calculus formation by inhibiting mineralization and crystal growth. 28,29 Vitamin C, a surface-active organophosphorous compound, has been shown 30,31 to inhibit calcium-phosphate crystallization in vitro.…”

Section: Mechanism Of Action Of Trk-530 In Calculus Inhibitionmentioning

confidence: 99%

Inhibitory Effect of a Novel Bisphosphonate, TRK‐530, on Dental Calculus Formation in Rats

Sikder

Itoh

Iwatsuki

et al. 2004

Journal of Periodontology

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Section: Mechanism Of Action Of Trk-530 In Calculus Inhibitionmentioning

confidence: 99%

Inhibitory Effect of a Novel Bisphosphonate, TRK‐530, on Dental Calculus Formation in Rats

Sikder

Itoh

Iwatsuki

et al. 2004

Journal of Periodontology

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“…coli can mineralize in liquid medium . Despite not being naturally present in mammalian saliva, β-glycerophosphate is traditionally used as a model for the organic source of phosphates in dental plaque calcification. , Two concentrations of calcium chloride were tested: a concentration of 1 mM corresponding to the typical ion concentration in human saliva , (experiment named P-Ca 10:1) and a concentration of 10 mM, which has previously been used to induce mineralization in liquid medium (experiment named P-Ca 10:10). Finally, salt-free LB agar was used as a control condition (nonmineralizing medium).…”

Section: Resultsmentioning

confidence: 99%

Induced Mineralization of Hydroxyapatite inEscherichia coliBiofilms and the Potential Role of Bacterial Alkaline Phosphatase

et al. 2023

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Biofilms appear when bacteria colonize a surface and synthesize and assemble extracellular matrix components. In addition to the organic matrix, some biofilms precipitate mineral particles such as calcium phosphate. While calcified biofilms induce diseases like periodontitis in physiological environments, they also inspire the engineering of living composites. Understanding mineralization mechanisms in biofilms will thus provide key knowledge for either inhibiting or promoting mineralization in these research fields. In this work, we study the mineralization of Escherichia coli biofilms using the strain E. coli K-12 W3110, known to produce an amyloid-based fibrous matrix. We first identify the mineralization conditions of biofilms grown on nutritive agar substrates supplemented with calcium ions and β-glycerophosphate. We then localize the mineral phase at different scales using light and scanning electron microscopy in wet conditions as well as X-ray microtomography. Wide-angle X-ray scattering enables us to further identify the mineral as being hydroxyapatite. Considering the major role played by the enzyme alkaline phosphatase (ALP) in calcium phosphate precipitation in mammalian bone tissue, we further test if periplasmic ALP expressed from the phoA gene in E. coli is involved in biofilm mineralization. We show that E. coli biofilms grown on mineralizing medium supplemented with an ALP inhibitor undergo less and delayed mineralization and that purified ALP deposited on mineralizing medium is sufficient to induce mineralization. These results suggest that also bacterial ALP, expressed in E. coli biofilms, can promote mineralization. Overall, knowledge about hydroxyapatite mineralization in E. coli biofilms will benefit the development of strategies against diseases involving calcified biofilms as well as the engineering of biofilm-based living composites.

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“…31 Despite not being naturally present in mammalian saliva, β-glycerophosphate is traditionally used as a model for organic phosphates in dental plaque calcification. 34,35 Two concentrations of calcium chloride were tested: a concentration of 1 mM corresponding to the typical ion concentration in human saliva 12,36 (experiment named P-Ca 10:1) and a concentration of 10 mM, that has previously been used to induce mineralization in liquid medium 31 (experiment named P-Ca 10:10). Finally, salt-free LB agar was used as a control condition (non-mineralizing medium).…”

Section: Resultsmentioning

confidence: 99%

Induced mineralization in Escherichia coli biofilms: the key role of bacterial alkaline phosphatase

Zorzetto

Scoppola

Raguin

et al. 2022

Preprint

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Biofilms appear when bacteria colonize a surface and synthesize and assemble extracellular matrix components. In addition to the organic matrix, some biofilms precipitate mineral particles such as calcium phosphate. While calcified biofilms induce diseases like periodontitis in physiological environments, they also inspire the engineering of living composites. Understanding mineralization mechanisms in biofilms will thus provide key knowledge for either inhibiting or promoting mineralization in these research fields. The enzyme alkaline phosphatase (ALP) plays a key role in calcium phosphate precipitation in mammalian bone tissue. Produced by eukaryotic cells, ALP catalyzes the hydrolysis of monophosphates starting from different precursors (e.g., alkaloids, proteins) and makes phosphate ions readily available for the precipitation with calcium. Bacterial ALPs are expressed by the well-characterized gram-negative and gram-positive bacteria E. coli and S. aureus as well as a large number of marine and soil bacteria. While it was recently proposed that bacterial ALPs induce mineral precipitation, their role in biofilm mineralization is not fully understood. In this work, we address this question using the biofilm-forming E. coli K-12 strain W3110, which expresses periplasmic ALP from the phoA gene. We first identify the mineralization conditions of biofilms grown on nutritive agar substrates supplemented with calcium ions and β-glycerophosphate. We then localize the mineral phase at different scales, using light and scanning electron microscopy as well as X-ray microtomography. Wide-angle X-ray scattering enables us to further identify the mineral as being hydroxyapatite. Finally, growing E. coli cells on mineralizing medium supplemented with an ALP inhibitor demonstrates that ALP is essential for biofilm mineralization. This is confirmed with a bacteria-free model, where the deposition of a drop of bacterial ALP solution on calcium and β-glycerophosphate containing agar substrate is sufficient to induce mineralization. Overall, these results will benefit the development of strategies against diseases involving calcified biofilms as well as the engineering of biofilm-based living composites.

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Crystal seeding by salivary calculus and its inhibition in vitro

Cited by 12 publications

References 7 publications

Inhibitory Effect of a Novel Bisphosphonate, TRK‐530, on Dental Calculus Formation in Rats

Inhibitory Effect of a Novel Bisphosphonate, TRK‐530, on Dental Calculus Formation in Rats

Induced Mineralization of Hydroxyapatite inEscherichia coliBiofilms and the Potential Role of Bacterial Alkaline Phosphatase

Induced mineralization in Escherichia coli biofilms: the key role of bacterial alkaline phosphatase

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