CryptoCEST: A promising tool for spatially resolved identification of fungal brain lesions and their differentiation from brain tumors with MRI

Vanherp, Liesbeth; Govaerts, Kristof; Riva, Mattéo; Poelmans, Jennifer; Coosemans, An; Lagrou, Katrien; Gsell, Willy; Velde, Greetje Vande

doi:10.1016/j.nicl.2021.102737

Cited by 5 publications

(5 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, increased local perfusion of the affected foot was noticed after amputation in patients with diabetic foot ulcers ( 1 ). Impaired microvascular permeability in tibial anterior muscles and larger T1-weighted signal characteristics of osteomyelitis were observed by DCE-MRI ( 10 , 11 ).…”

Section: Discussionmentioning

confidence: 90%

“…Because of the wide range of CEST effect, it will also affect the signal at MTRasym (3.5 ppm) and lead to its indirect increase. For the same reason, the CryptoCEST contrast at 4 ppm ( 11 ) will also lead to an elevated MTRasym (3.5ppm). Third, technical factors such as non-uniform B1 field and motion during scanning ( 19 ) could also lead to abnormal increase in signal at MTRasym (3.5 ppm).…”

Section: Discussionmentioning

confidence: 99%

“…BacCEST MRI makes it possible to identify, classify, and keep track of bacterial infections in rat brain with a bacterial abscess ( 10 ). CryptoCEST contrast enabled the detection of cryptococcomas in the brains of mice ( 11 ). The necrotic tissue and interstitial exudate from the DF lesions are rich in protein.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Amide proton transfer weighted contrast has diagnostic capacity in detecting diabetic foot: an MRI-based case–control study

Lu,

Tian,

Zhao

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

ObjectiveTo evaluate the role of foot muscle amide proton transfer weighted (APTw) contrast and tissue rest perfusion in quantifying diabetic foot (DF) infection and its correlation with blood parameters.Materials and methodsWith approval from an ethical review board, this study included 40 diabetes mellitus (DM) patients with DF and 31 DM patients without DF or other lower extremity arterial disease. All subjects underwent MRI, which included foot sagittal APTw and coronal arterial spin labeling (ASL) imaging. The normalized MTRasym (3.5 ppm) and the ratio of blood flow (rBF) in rest status of the affected side lesions to the non-affected contralateral side were determined. The inter-group differences of these variables were evaluated. Furthermore, the association between normalized MTRasym (3.5 ppm), rBF, and blood parameters [fasting blood glucose (FBG), glycosylated hemoglobin content, C-reactive protein, neutrophil percentage, and white blood cell count] was explored. Using an ROC curve, the diagnostic capacity of normalized MTRasym (3.5 ppm), BF, and blood biochemical markers in differentiating with or without DF in DM was assessed.ResultsIn the DF group, MTRasym (3.5 ppm) and BF in lesion and normalized MTRasym (3.5 ppm) were higher than those in the control group (p < 0.05). In addition, correlations were identified between normalized MTRasym (3.5 ppm) and blood parameters, such as C-reactive protein, glycosylated hemoglobin content, FBG, neutrophil ratio, and white blood cell (p < 0.001). Meanwhile, association between BF in lesion and blood parameters, such as C-reactive protein, neutrophil percentage, and FBG (p < 0.01). AUC of normalized MTRasym (3.5 ppm) in identifying with/without DF in patients with DM is 0.986 (95% CI, 0.918–1.00) with the sensitivity of 97.22% and the specificity of 100%.ConclusionNormalized MTRasym (3.5 ppm) and the BF in lesion may be treated as a safer and more convenient new indicator to evaluate the tissue infection without using a contrast agent, which may be useful in monitoring and preoperatively assessing DF patients with renal insufficiency.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Amide proton transfer weighted contrast has diagnostic capacity in detecting diabetic foot: an MRI-based case–control study

Lu,

Tian,

Zhao

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…Trehalose can be identified by in vivo MR spectroscopy in animal models and in patients with largesized cryptococcoma [168]. The use of trehalose as a marker compound has been further optimized in so-called CEST MRI protocols, which also allow for the identification and quantification of trehalose in small lesions [289]. The in vivo quantification of trehalose is Trehalose and mannitol are compounds that are not produced in high concentrations in mammalian tissue or abscess-causing microorganisms but by CN and CG.…”

Section: Magnetic Resonance Imaging and Spectroscopymentioning

confidence: 99%

Preclinical Models for Cryptococcosis of the CNS and Their Characterization Using In Vivo Imaging Techniques

Roosen,

Maes,

Musetta

et al. 2024

JoF

Self Cite

View full text Add to dashboard Cite

Infections caused by Cryptococcus neoformans and Cryptococcus gattii remain a challenge to our healthcare systems as they are still difficult to treat. In order to improve treatment success, in particular for infections that have disseminated to the central nervous system, a better understanding of the disease is needed, addressing questions like how it evolves from a pulmonary to a brain disease and how novel treatment approaches can be developed and validated. This requires not only clinical research and research on the microorganisms in a laboratory environment but also preclinical models in order to study cryptococci in the host. We provide an overview of available preclinical models, with particular emphasis on models of cryptococcosis in rodents. In order to further improve the characterization of rodent models, in particular the dynamic aspects of disease manifestation, development, and ultimate treatment, preclinical in vivo imaging methods are increasingly used, mainly in research for oncological, neurological, and cardiac diseases. In vivo imaging applications for fungal infections are rather sparse. A second aspect of this review is how research on models of cryptococcosis can benefit from in vivo imaging methods that not only provide information on morphology and tissue structure but also on function, metabolism, and cellular properties in a non-invasive way.

show abstract

“…Recent studies found that Chemical Exchange Saturation Transfer (CEST) MRI might be promising to assess diagnosis whenever morphological study and MRS fail. This technique detects low concentrations of targeted substrates, such as amide acid, to detect protein rings around bacterial abscesses or trehalose sugar content in fungal abscesses [24,25]. As for nuclear imaging, 18 F-fluorodeoxyglucose (FDG)-PET/computed tomography (CT) suffers from a lack of specificity, as does CT scan.…”

Section: Neuroimagingmentioning

confidence: 99%

Brain abscess in immunocompetent patients: recent findings

Thy

Gaudemer²,

d’Humières

et al. 2022

Current Opinion in Infectious Diseases

View full text Add to dashboard Cite

Purpose of reviewWe conducted a systematic review of the literature to update findings on the epidemiology and the management of cerebral abscesses in immunocompetent patients.Recent findingsObservational studies suggest that the overall prognosis has improved over the last decades but mortality rates remain high. Several parameters may contribute to a better prognosis, including the identification of common risk factors for brain abscess, the systematic use of brain MRI at diagnosis, the implementation of appropriate neurosurgical and microbiological techniques for diagnosis, the optimization of the antibacterial treatment based on epidemiology and pharmacokinetic/pharmacodynamic studies, and a long-term follow-up for detection of secondary complications. Outcome research on brain abscess is mainly based on observational studies. Randomized controlled trials have yet to be performed to identify clinically relevant interventions associated with improved patient-centered outcomes.SummaryOur review highlights the importance of a multidisciplinary approach to optimize brain abscess management both at the acute phase and in the long-term. Randomized controlled studies are urgently needed to identify interventions associated with improved outcomes.

show abstract

CryptoCEST: A promising tool for spatially resolved identification of fungal brain lesions and their differentiation from brain tumors with MRI

Cited by 5 publications

References 62 publications

Amide proton transfer weighted contrast has diagnostic capacity in detecting diabetic foot: an MRI-based case–control study

Amide proton transfer weighted contrast has diagnostic capacity in detecting diabetic foot: an MRI-based case–control study

Preclinical Models for Cryptococcosis of the CNS and Their Characterization Using In Vivo Imaging Techniques

Brain abscess in immunocompetent patients: recent findings

Contact Info

Product

Resources

About