Cryptocandin, a potent antimycotic from the endophytic fungus Cryptosporiopsis cf. quercina

Strobel, Gary A.; Miller, Russell; Martinez-Miller, C.; Condron, Margaret M.; Teplow, David B.; Hess, W. M.

doi:10.1099/13500872-145-8-1919

Cited by 222 publications

(115 citation statements)

References 3 publications

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“…Podem ser utilizados como vetores para introdução de genes de interesse nas plantas (Fahey, 1988;Murray et al, 1992), como agentes inibidores de pragas e patógenos (Volksch et al, 1992;Hallmann & Sikora, 1996) e como fontes de metabólitos primários (Stamford et al, 1998) e secundários de interesse como o taxol, poderoso anticancerígeno (Stierle et al, 1993;Wang et al, 2000), a cryptocandina, lipopeptídeo antimicótico (Strobel et al, 1999) e diversos outros antibióticos. Relatos como o do taxol, inicialmente isolado de Taxus brevifolia e, em seguida, de diversos endófitos desta e de outras plantas que o produzem, sugerem um relacionamento entre planta e microrganismo que deve ser melhor explorado.…”

Section: Introductionunclassified

Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl.) rich e Strychnos cogens bentham

et al. 2004

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RESUMODas plantas tóxicas da Amazônia Palicourea longiflora e Strychnos cogens foram isolados 571 fungos endofíticos e 74 bactérias endofíticas. Palicourea longiflora (Rubiaceae) e outras espécies desse gênero estão relacionadas a 90% das mortes de gado na região Amazônica. Strychnos cogens (Loganiaceae) e outras espécies de Strychnos são utilizadas pelos indígenas na confecção de "curares". Entre os endófitos isolados de P. longiflora foram identificados os fungos: Colletotrichum sp. e seu telemorfo Glomerella sp., Guignardia sp., Aspergillus niger, Phomopsis sp. e Xylaria sp., além da bactéria Burkholderia gladioli, pertencente a um grupo de fixadoras de nitrogênio. Dos isolados de S. cogens foram identificados os fungos: Colletotrichum sp., Guignardia sp., Aspergillus niger e Trichoderma sp. Uma amostra de 79 isolados fúngicos teve seus metabólitos extracelulares bioensaiados contra microrganismos patogênicos e fitopatogênicos: 19 isolados inibiram um ou mais microrganismos-teste. Dos oito isolados com melhores resultados de inibição, as móleculas bioativas eram menores que 12.000 daltons, fato verificado pela diálise dos metabólitos. PALAVRAS-CHAVEPalicourea longiflora, Strychnos cogens, fungos endofíticos, bactérias endofíticas, bioensaios. Antimicrobial activity of endophytic fungi isolated from

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Section: Introductionunclassified

Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl.) rich e Strychnos cogens bentham

et al. 2004

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“…and Pezicula sp. in Pinus sylvestris and Fagus sylvatica (Noble et al, 1991) and the antifungal cyclopeptide cryptocandin from the endophytic Cryptosporiopsis compared with quercina of redwood (Strobel et al, 1999). We are currently isolating the oligopeptide identified in this study from endophyteinfected perennial ryegrass to elucidate its structure and to test its potential antimicrobial activity.…”

Section: Biological Implications Of Identified Metabolitesmentioning

confidence: 99%

Advanced Data-Mining Strategies for the Analysis of Direct-Infusion Ion Trap Mass Spectrometry Data from the Association of Perennial Ryegrass with Its Endophytic Fungus,Neotyphodium lolii

et al. 2008

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Direct-infusion mass spectrometry (MS) was applied to study the metabolic effects of the symbiosis between the endophytic fungus Neotyphodium lolii and its host perennial ryegrass (Lolium perenne) in three different tissues (immature leaf, blade, and sheath). Unbiased direct-infusion MS using a linear ion trap mass spectrometer allowed metabolic effects to be determined free of any preconceptions and in a high-throughput fashion. Not only the full MS 1 mass spectra (range 150-1,000 mass-to-charge ratio) were obtained but also MS 2 and MS 3 product ion spectra were collected on the most intense MS 1 ions as described previously (Koulman et al., 2007b). We developed a novel computational methodology to take advantage of the MS 2 product ion spectra collected. Several heterogeneous MS 1 bins (different MS 2 spectra from the same nominal MS 1 ) were identified with this method. Exploratory data analysis approaches were also developed to investigate how the metabolome differs in perennial ryegrass infected with N. lolii in comparison to uninfected perennial ryegrass. As well as some known fungal metabolites like peramine and mannitol, several novel metabolites involved in the symbiosis, including putative cyclic oligopeptides, were identified. Correlation network analysis revealed a group of structurally related oligosaccharides, which differed significantly in concentration in perennial ryegrass sheaths due to endophyte infection. This study demonstrates the potential of the combination of unbiased metabolite profiling using ion trap MS and advanced data-mining strategies for discovering unexpected perturbations of the metabolome, and generating new scientific questions for more detailed investigations in the future.With the advent of metabolomics, methods for the simultaneous analysis of a large number of small molecules (metabolites) have been developed and improved, providing more details about the metabolism of complex biological systems (Sumner et al., 2003;Dettmer et al., 2007). Metabolite fingerprinting methods provide relatively unbiased and high-throughput information on complex biological systems and have been used for yeast (Saccharomyces cerevisiae) strain classification (Allen et al., 2003) and annotation of gene functions (Raamsdonk et al., 2001). Comprehensive and unbiased metabolite analysis is also an indispensable tool for systems biology together with transcriptomics and proteomics (see commentary by Sauer et al., 2007). Direct-infusion (without prior chromatographic separation) electrospray ionization (ESI) mass spectrometry (MS) was introduced as a tool for the identification of novel fungal metabolites in culture (Smedsgaard and Frisvad, 1996) and is now widely applied in metabolomics (for a recent review, see Dettmer et al., 2007). High resolution MS instrumentation such as time-of-flight MS (Dunn et al., 2005) or Fourier transform ion cyclotron resonance MS (FT-ICR-MS; Aharoni et al., 2002) is often preferred for the specificity provided by resolution of isobaric ions and highly accurate e...

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“…Cryptosporiopsis quercina is the imperfect stage of Pezicula cinamomea, a fungus commonly associated with the hardwood species in Europe. It was isolated by Strobel et al (1999) as an endophyte from Tripterigeum wilfordii, a medicinal plant native to Eurasia. On Petri plates, C. quercina exhibited excellent antifungal activity against C. albicans.…”

Section: Secondarymentioning

confidence: 99%

Endophytes: exploitation as a tool in plant protection

Dutta

Puzari

Gogoi

et al. 2014

Braz. arch. biol. technol.

139

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Cryptocandin, a potent antimycotic from the endophytic fungus Cryptosporiopsis cf. quercina

Cited by 222 publications

References 3 publications

Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl.) rich e Strychnos cogens bentham

Atividade antimicrobiana de fungos endofíticos isolados de plantas tóxicas da amazônia: Palicourea longiflora (aubl.) rich e Strychnos cogens bentham

Advanced Data-Mining Strategies for the Analysis of Direct-Infusion Ion Trap Mass Spectrometry Data from the Association of Perennial Ryegrass with Its Endophytic Fungus,Neotyphodium lolii

Endophytes: exploitation as a tool in plant protection

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