Cryptic Plasmids in Hospital Isolates of Providencia Stuarti

Hawkey, Peter M.; Bennett, PM; Hawkey, Carol A.

doi:10.1099/00222615-18-2-277

Cited by 14 publications

(8 citation statements)

References 8 publications

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“…stuartii. (Hawkey, Bennet & Hawkey, 1984;Hollick et al 1984). Thus although plasmid profiles may be useful epidemiological markers, considerable care is required in using them to interpret the movement of strains.…”

Section: Discussionmentioning

confidence: 99%

Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Hawkey

Penner

Linton

et al. 1986

J. Hyg.

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rettgeri. A wide range of serotypes was found, many having been previously reported from nosocomial isolates. A total of 15 % of isolates carried plasmids; six pairs of isolates were identified which had identical serotypes but different patterns of plasmid carriage. The antimicrobial sensitivity of the isolates was generally similar to isolates of Proteeae from humans. Although no truly aminoglycosideresistant isolates were found, some isolates of Prov. stuartii and Prov. rettgeri had MIC's higher than the other isolates to gentamicin and netilmicin, suggesting the presence of low levels of the enzyme AAC 2'. The study demonstrates that there is a considerable diversity of species and types of Proteeae associated with calves and their environment. It seems likely that a potential cause of colonization of the human gut by Proteeae is the consumption of meat.

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Section: Discussionmentioning

confidence: 99%

Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Hawkey

Penner

Linton

et al. 1986

J. Hyg.

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“…Plasmids have been reported in Providencia stuartii (79,132), as well as in Providencia rettgeri (93). Urease production, sucrose utilization, and lactose fermentation can be plasmid mediated in Providencia stuartii (132).…”

Section: Other Enteric Bacteria and Miscellaneousmentioning

confidence: 99%

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Mayer

1988

Clin Microbiol Rev

151

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Plasmids are circular deoxyribonucleic acid molecules that exist in bacteria, usually independent of the chromosome. The study of plasmids is important to medical microbiology because plasmids can encode genes for antibiotic resistance or virulence factors. Plasmids can also serve as markers of various bacterial strains when a typing system referred to as plasmid profiling, or plasmid fingerprinting is used. In these methods partially purified plasma deoxyribonucleic acid species are separated according to molecular size by agarose gel electrophoresis. In a second procedure, plasmid deoxyribonucleic acid which has been cleaved by restriction endonucleases can be separated by agarose gel electrophoresis and the resulting pattern of fragments can be used to verify the identity of bacterial isolates. Because many species of bacteria contain plasmids, plasmid profile typing has been used to investigate outbreaks of many bacterial diseases and to trace inter- and intra-species spread of antibiotic resistance.

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“…More recently, this approach has been widely used to study the epidemiology of both plasmids and their host bacteria (Beul et al 1985;Lyon et al 1984; O'Brien et al 1982;Platt, Chesham & Kristinsson, 1986;Rubens et al 1981). The information provided by the application of these techniques is particularly useful for the study of genera for which no established typing scheme is available (Hawkey, Bennett & Hawkey, 1984) and the investigation of cryptic plasmids (Callihan, Young & Clark, 1983). Furthermore, the fingerprints may contain fragments characteristic of individual phenotypic traits specified by the plasmid (Platt, 1983).…”

Section: Introductionmentioning

confidence: 99%

“…(1984), EcoRI, HpaII and HindIII to investigate staphylococci, and Hawkey, Bennett & Hawkey (1984) digested Providencia stuartii plasmids with EcoRl and HinCII. Thus, it is not possible at present to compare plasmids from different genera or different laboratories without employing a wide range of enzymes, and the purification of large quantities of DNA is required from each strain.…”

Section: Introductionmentioning

confidence: 99%

Restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application

Platt

Chesham

Brown

et al. 1986

J. Hyg.

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SUMMARYRestriction enzyme fingerprints were generated from purified plasmid DNA from 324 clinical isolates that belonged to 7 enterobacterial genera and 88 single plasmids in Escherichia coli K 12 according to the following strategy.Purified plasmid DNA was digested with PstI. The number of fragments detected in a 0-8 agarose gel was used to determine which 2 of 6 restriction enzymes including PstI was most likely to provide a fingerprint comprising sufficient fragments to ensure specificity but sufficiently few to allow easy visual assessment and minimize coincidental matching. When Pst I produced > 20 fragments, Eco RI and Hind III were used; when PstI generated < 6 fragments Bsp 1286 and AvaII were used and SmaI was employed when between 6 and 20 fragments were obtained from PstI digests. Using a minimum of 12 fragments from a combination of 2 enzymes as the criterion for characterizing a strain/plasmid, satisfactory 2-enzyme fingerprints were obtained from 87 % of the strains and plasmids studied using Pst I and no more than two additional enzymes per strain. Of the remaining 54 strains, 51 harboured only small plasmids (< 10 kb) and 3 produced satisfactory fingerprints when digested with a fourth enzyme.

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Cryptic Plasmids in Hospital Isolates of Providencia Stuarti

Cited by 14 publications

References 8 publications

Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application

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