Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Hawkey, Peter M.; Penner, J L; Linton, A. H.; Hawkey, Carol A.; Crisp, L J; Hinton, M.

doi:10.1017/s0022172400063592

Cited by 14 publications

(6 citation statements)

References 34 publications

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“…As few as 10 % of the strains tested contained plasmid DNA. A similar rate (15 %) has been reported for Proteae isolated from animal sources although that study did not include Proteus penneri (11). In our study only ampiciltin and tetracycline resistance markers could be transferred to Escherichia coli.…”

Section: Methodssupporting

confidence: 84%

Analysis of antibiotic resistance determinants inProteus penneri

Łukomski

Pytlos

Serwecińska

et al. 1993

Eur. J. Clin. Microbiol. Infect. Dis.

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The plasmid profiles of 65 strains of Proteus penneri were analyzed to determine whether resistance was determined chromosomally or by plasmids. Only seven strains harboured one to three plasmids, although these strains exhibited resistance to a wide range of antibiotics. Markers for ampicillin and tetracycline resistance could be transferred to Escherichia coli by transformation. Plasmids carried resistance to chloramphenicol in two strains and resistance to sulfonamides in one strain. The result showed that resistance is determined chromosomally rather than by plasmids, however the possibility that these bacteria may acquire resistance plasmids which change their antibiotic susceptibility pattern cannot be excluded.

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Section: Methodssupporting

confidence: 84%

Analysis of antibiotic resistance determinants inProteus penneri

Łukomski

Pytlos

Serwecińska

et al. 1993

Eur. J. Clin. Microbiol. Infect. Dis.

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“…Hawkey et al [56] revealed that P. vulgaris (including the strains currently numbered among genomospecies) as well as P. mirabilis species were, respectively, the first and the third species among the tribe Proteeae most commonly isolated from beddings contaminated with feces and urine in two calf farms in South West England. The authors concluded that the high similarity of the O-serotype profile of isolated strains (e.g., serotypes O23 and O30) to those reported for human infections (Table 2) suggests that food animals may be a source of Proteeae strains carried in human gut.…”

Section: Proteus Spp In Animals—adverse and Friendlymentioning

confidence: 99%

Significance and Roles of Proteus spp. Bacteria in Natural Environments

2016

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Proteus spp. bacteria were first described in 1885 by Gustav Hauser, who had revealed their feature of intensive swarming growth. Currently, the genus is divided into Proteus mirabilis, Proteus vulgaris, Proteus penneri, Proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 O-antigenic serogroups. The bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. It is postulated that intestines are a reservoir of these proteolytic organisms. Many wild and domestic animals may be hosts of Proteus spp. bacteria, which are commonly known to play a role of parasites or commensals. However, interesting examples of their symbiotic relationships with higher organisms have also been described. Proteus spp. bacteria present in soil or water habitats are often regarded as indicators of fecal pollution, posing a threat of poisoning when the contaminated water or seafood is consumed. The health risk may also be connected with drug-resistant strains sourcing from intestines. Positive aspects of the bacteria presence in water and soil are connected with exceptional features displayed by autochthonic Proteus spp. strains detected in these environments. These rods acquire various metabolic abilities allowing their adaptation to different environmental conditions, such as high concentrations of heavy metals or toxic substances, which may be exploited as sources of energy and nutrition by the bacteria. The Proteus spp. abilities to tolerate or utilize polluting compounds as well as promote plant growth provide a possibility of employing these microorganisms in bioremediation and environmental protection.

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“…Serotyping, bacteriocin and bacteriophage typing, numerical analysis of electrophoretic protein patterns, plasmids, and antibiotic resistance pattern determination have been used for typing some species and have been applied to epidemiological investigations with variable efficiency (2,7,12,17,20,25). In more recent years, molecular typing by ribosomal DNA (rDNA) fingerprints (ribotyping), based on restriction fragment length polymorphism in the chromosomal DNA containing rRNA genes (9,18), has been applied for the epidemiological study of P. stuartii clinical isolates (19).…”

mentioning

confidence: 99%

Molecular Characterization of the Genera Proteus , Morganella , and Providencia by Ribotyping

et al. 1999

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The so-called Proteus-Providencia group is constituted at present by three genera and 10 species. Several of the recognized species are common opportunistic pathogens for humans and animals. Different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. We have determined the rRNA gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera Proteus,Morganella, and Providencia. Visual inspection of EcoRV- and HincII-digested DNA from the type strains showed remarkably different patterns for both enzymes, butEcoRV provided better differentiation. Both endonucleases were retained to study a large number of wild and collection strains belonging to the different species. Clinical isolates of Proteus mirabilis, Proteus penneri, Morganella morganii, and Providencia heimbachae showed patterns identical or very similar to those of the respective type strains, so that groups of related patterns (ribogroups) were found to correspond to the diverse species. On the contrary, distinct ribogroups were detected within Providencia alcalifaciens (two ribogroups with both enzymes), Providencia rettgeri (four ribogroups with EcoRV and five with HincII),Providencia stuartii (two ribogroups withEcoRV), Providencia rustigianii (two ribogroups with HincII), and Proteus vulgaris (two ribogroups with both enzymes). The pattern shown by the ancientP. vulgaris type strain NCTC 4175 differed considerably from both P. vulgaris ribogroups as well as from the newly proposed type strain ATCC 29905 and from any other strain in this study, thus confirming its atypical nature. Minor differences were frequently observed among patterns of strains belonging to the same ribogroup. These differences were assumed to define ribotypes within each ribogroup. No correlation was observed between ribogroups or ribotypes and biogroups of P. vulgaris, P. alcalifaciens, P. stuartii, and P. rettgeri. Since, not only different species showed different rRNA gene restriction patterns, but also different ribogroups and ribotypes have been found in the majority of the species, ribotyping would be a sensitive method for molecular characterization of clinical isolates belonging to the genera Proteus, Morganella, and Providencia.

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Speciation, serotyping, antimicrobial sensitivity and plasmid content of Proteeae from the environment of calf-rearing units in South West England

Cited by 14 publications

References 34 publications

Analysis of antibiotic resistance determinants inProteus penneri

Analysis of antibiotic resistance determinants inProteus penneri

Significance and Roles of Proteus spp. Bacteria in Natural Environments

Molecular Characterization of the Genera Proteus , Morganella , and Providencia by Ribotyping

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