Cryothermic and Hyperthermic Treatments of Human Leiomyomata and Adjacent Myometrium and Their Implications for Laparoscopic Surgery

Rupp, Christopher C.; Nagel, Theodore C.; Swanlund, David J.; Bischof, John C.; Coad, James E.

doi:10.1016/s1074-3804(05)60241-1

Cited by 20 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As previously reported in an in vitro study, in part cryothermic treatment produces irreversibile cell injury or death on leiomyoma smooth muscle at temperatures of )20°to )80°C with a 15-min hold. Although complete direct ablation of the leiomyoma smooth muscle is not detected with direct immersion in LN2 ()196°C), additional factors such as ischemic injury from vascular thrombosis may have to be exploited for complete cryothermic ablation of leiomyomata [17]. T 0 , volume before cryomyolysis; T 1 , 6 months of follow-up; T 2 , 12 months of follow-up * p < 0.05 ** p < 0.001 T 0 , volume before cryomyolysis; T 2 , 12 months of follow-up…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic cryomyolysis: An alternative to myomectomy in women with symptomatic fibroids

et al. 2004

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Laparoscopic cryomyolysis: An alternative to myomectomy in women with symptomatic fibroids

et al. 2004

View full text Add to dashboard Cite

show abstract

“…This led to the selection of 300 mm as the thickness of the tissue slices that would allow for adequate culture. However, one has had good success using with this culture technique to assess the evolution of tissue injury after cryothermic or hyperthermic treatment with much thicker (1-3 mm) tissue slices under various culture conditions 24,27,32 .…”

Section: Validation Of Tissue Slicing and Culture Methodsmentioning

confidence: 99%

“…Dye uptake analyses were performed after thermal treatment, as described by Koushafar et al 26 ; however, injury was assessed after 3, 24 and 72 h of culture using the method outlined in Rupp et al 27 . In brief, the tumour slices were incubated for 30 min in 1 ml of Dulbecco's phosphate-buffered saline (Invitrogen; Gaithersburg, MD) with 9 mM Hoechst 33342 (Sigma) and 16 mM EthD-1 (Molecular Probes; Eugene, OR).…”

Section: Dye Uptakementioning

confidence: 99%

“…However, the exact point where cells can be definitively classified as dead (without obvious nuclear degradation) is still not clear. This prompted the use of an additional viability assay, notably quantitative dye uptake analysis, which was previously used as a marker of cell viability after treatment at temperatures relevant to thermal therapy 14,27 .…”

Section: Comparison Of Injury Assaysmentioning

confidence: 99%

See 1 more Smart Citation

In vitrothermal therapy of AT-1 Dunning prostate tumours

Bhowmick

Coad

Swanlund

et al. 2004

International Journal of Hyperthermia

View full text Add to dashboard Cite

To advance the utility of prostate thermal therapy, this study investigated the thermal thresholds (temperature-time) for prostate tissue destruction in vitro. The AT-1 Dunning prostate tumour model was chosen for the study. Three hundred micron thick sections were subjected to controlled temperature-time heating, which ranged from low (40 degrees C, 15 min) to high thermal exposures (70 degrees C, 2 min) (n = 6). After subsequent tissue culture at 37 degrees C, the sections were evaluated for tissue injury at 3, 24 and 72 h by two independent methods: histology and dye uptake. A graded increase in injury was identified between the low and high thermal exposures. Maximum histologic injury occurred above 70 degrees C, 1 min with >95% of the tissue area undergoing significant cell injury and coagulative necrosis. The control and 40 degrees C, 15 min sections showed histologic evidence of apoptosis following 24 and 72 h in culture. Similar signs of apoptosis were minimal or absent at higher thermal histories. Vital-dye uptake quantitatively confirmed complete cell death after 70 degrees C, 2 min. Using the dye data, Arrhenius analysis showed an apparent breakpoint at 50 degrees C, with activation energies of 135.8 kcal/mole below and 4.7 kcal/mole above the threshold after 3 h in culture. These results can be used as a conservative benchmark for thermal injury in the cancerous prostate. Further characterization of the response to thermal therapy in an animal model and in human tissues will be important in establishing the efficacy of the procedure

show abstract

“…Use of 20°C instead of 37°C allows for the irreversibly injured cells to manifest damage histologically, while uninjured cells appear histologically viable. 25,26,34 The concentrations were chosen such that osmotic stress on the cells did not induce SMC death.…”

Section: Osmotic (Mannitol) Treatmentmentioning

confidence: 99%

Freeze–Thaw Induced Biomechanical Changes in Arteries: Role of Collagen Matrix and Smooth Muscle Cells

et al. 2010

Self Cite

View full text Add to dashboard Cite

Applications involving freeze-thaw, such as cryoplasty or cryopreservation can significantly alter artery biomechanics including an increase in physiological elastic modulus. Since artery biomechanics plays a significant role in hemodynamics, it is important to understand the mechanisms underlying these changes to be able to help control the biomechanical outcome post-treatments. Understanding of these mechanisms requires investigation of the freeze-thaw effect on arterial components (collagen, smooth muscle cells or SMCs), as well as the components' contribution to the overall artery biomechanics. To do this, isolated fresh swine arteries were subjected to thermal (freeze-thaw to -20 degrees C for 2 min or hyperthermia to 43 degrees C for 2 h) and osmotic (0.1-0.2 M mannitol) treatments; these treatments preferentially altered either the collagen matrix (hydration/stability) or smooth muscle cells (SMCs), respectively. Tissue dehydration, thermal stability and SMC functional changes were assessed from bulk weight measurements, analyses of the thermal denaturation profiles using Fourier transform infrared (FTIR) spectroscopy and in vitro arterial contraction/relaxation responses to norepinephrine (NE) and acetylcholine (AC), respectively. Additionally, Second Harmonic Generation (SHG) microscopy was performed on fresh and frozen-thawed arteries to directly visualize the changes in collagen matrix following freeze-thaw. Finally, the overall artery biomechanics was studied by assessing responses to uniaxial tensile testing. Freeze-thaw of arteries caused: (a) tissue dehydration (15% weight reduction), (b) increase in thermal stability (approximately 6.4 degrees C increase in denaturation onset temperature), (c) altered matrix arrangement observed using SHG and d) complete SMC destruction. While hyperthermia treatment also caused complete SMC destruction, no tissue dehydration was observed. On the other hand, while 0.2 M mannitol treatment significantly increased the thermal stability (approximately 4.8 degrees C increase in denaturation onset), 0.1 M mannitol treatment did not result in any significant change. Both 0.1 and 0.2 M treatments caused no change in SMC function. Finally, freeze-thaw (506+/-159 kPa), hyperthermia (268+/-132 kPa) and 0.2 M mannitol (304+/-125 kPa) treatments all caused significant increase in the physiological elastic modulus (Eartery) compared to control (185+/-92 kPa) with the freeze-thaw resulting in the highest modulus. These studies suggest that changes in collagen matrix arrangement due to dehydration as well as SMC destruction occurring during freeze-thaw are important mechanisms of freeze-thaw induced biomechanical changes.

show abstract

Cryothermic and Hyperthermic Treatments of Human Leiomyomata and Adjacent Myometrium and Their Implications for Laparoscopic Surgery

Cited by 20 publications

References 22 publications

Laparoscopic cryomyolysis: An alternative to myomectomy in women with symptomatic fibroids

Laparoscopic cryomyolysis: An alternative to myomectomy in women with symptomatic fibroids

In vitrothermal therapy of AT-1 Dunning prostate tumours

Freeze–Thaw Induced Biomechanical Changes in Arteries: Role of Collagen Matrix and Smooth Muscle Cells

Contact Info

Product

Resources

About