Cryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA)

Pereira, Rúben; Baptista, M.C.; Vasques, M.I.; Horta, A.E.M.; Portugal, P.V.; Bessa, Rui J. B.; Silva, J. Chagas e; Pereira, Martinha; Marques, C.C.

doi:10.1016/j.anireprosci.2006.03.015

Cited by 82 publications

(98 citation statements)

References 31 publications

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“…Here it was shown that after 44 to 48 h of culture, t10,c12 CLA oocytes were lighter (dark gray 5 55.6% and light gray 5 44.4%) than those of control and forskolin treatments (dark gray 5 100%). Reports confirm the reduction of lipid accumulation in in vitro embryos (Pereira et al, 2007 and or the decrease in lipogenesis of porcine adipose explants (José et al, 2008) after culture with this CLA isomer. Moreover, the presence of t10,c12 CLA during maturation improved bovine oocyte developmental competence (Lapa et al, 2011).…”

Section: Discussionmentioning

confidence: 69%

“…Trans-10, cis-12 octadecadienoic acid (100 mM t10,c12 CLA, Matreya, Pleasant Gap, Pennsylvania, USA; Pereira et al, 2007) and forskolin (10 mM, Men et al, 2006) were first diluted in ethanol because of its hydrophobicity and added to oocytes in the maturation media using different supplementation times according to the following experiments. The final concentration of ethanol was adjusted to 0.1% in all treatment groups.…”

Section: Experimental Designmentioning

confidence: 99%

“…Recently, two chemicals, trans-10, cis-12 conjugated linoleic acid (t10,c12 CLA) and forskolin, intracellular lipid modulators, were shown to improve porcine and bovine embryo survival after cryopreservation (Men et al, 2006;Pereira et al, 2007 and. Indeed, in vitro-produced bovine embryos cultured with 100 mM t10,c12 CLA were characterized by a reduced lipid accumulation and an improved blastocyst cryosurvival (Pereira et al, 2007 and.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, in vitro-produced bovine embryos cultured with 100 mM t10,c12 CLA were characterized by a reduced lipid accumulation and an improved blastocyst cryosurvival (Pereira et al, 2007 and. Moreover, alterations in the lipid profile of bovine oocytes matured with this CLA isomer were associated with an enhanced blastocyst quality (Lapa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

Prates

Marques

Baptista

et al. 2013

Animal

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Lipid droplets (LD) in porcine oocytes form a dark mass reaching almost all cytoplasm. Herein we investigated changes in fat areas, cytoplasmic tone and LD morphology duringin vitromaturation (IVM) of porcine oocytes cultured with 100 μMtrans-10,cis-12 conjugated linoleic acid (t10,c12 CLA) or 10 μM forskolin at different time periods. Four groups were constituted: control, excipient,t10,c12 CLA and forskolin, with drugs being supplemented during 44 to 48 h and the initial 22 to 24 h in Experiments 1 and 2, respectively. In Experiment 3, forskolin was supplemented for the first 2 h. Matured oocytes were inseminated with frozen-thawed boar semen and cleavage rate recorded. Before and during IVM, samples of oocytes were evaluated for LD, total and fat areas and fat gray value or for meiotic progression. Results showed that forskolin supplementation during 44 to 48 h or 22 to 24 h inhibits oocyte maturation (exp. 1: forskolin = 5.1 ± 8.0%, control = 72.6 ± 5.0%; exp. 2: forskolin = 24.3 ± 7.4%, control = 71.6 ± 5.6%) and cleavage (exp. 1: forskolin = 0.0 ± 0.0%, control = 55.4 ± 4.1%; exp. 2: forskolin = 8.3 ± 3.3%, control = 54.5 ± 3.0%). Forskolin also reduced oocyte and fat areas. In Experiment 3, forskolin negative effect on oocyte maturation and cleavage disappeared, although minor (P⩽ 0.03) LD and oocyte fat areas were identified at 22 to 24 h of IVM. Oocytes supplemented witht10,c12 CLA during 44 to 48 h presented a lighter (P⩽ 0.04) colour tone cytoplasm than those of control and forskolin. In conclusion,t10,c12 CLA and forskolin were capable of modifying the distribution and morphology of cytoplasmic LD during porcine oocyte maturation, thus reducing its lipid content in a time-dependent manner.

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Section: Discussionmentioning

confidence: 69%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

Prates

Marques

Baptista

et al. 2013

Animal

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“…Research has demonstrated that linoleic acid and oleic acid have antioxidant properties and are thus required for animal growth and animal health (13,14). It has been determined that following the freezing and thawing of bovine embryos, the supplementation of their culture medium with trans-10, cis-12 octadecadienoic acid (t10, c12 CLA), a conjugated linoleic acid derivative, increases the viability of embryos (15). The low survival capability of the embryos results in low rates of gestation and limits the use of in vitro-derived bovine embryos (16).…”

Section: Introductionmentioning

confidence: 99%

The effect of oleic and linoleic acids on in vitro bovineembryonic development and embryo quality

Karaşahi̇n¹,

Arıkan²

2015

Turk J Vet Anim Sci

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IntroductionIt has been proven that the morphological and physiological quality of in vivo-derived embryos is better than that of in vitro-derived embryos (1,2). One of the major reasons for the relative inadequacy of in vitro embryonic development is the generation of reactive oxygen species (ROS) (2). ROS generation occurs as a result of the reduction of free oxygen during the metabolism of molecular oxygen required for embryonic development. The major ROS produced by the embryo itself are the superoxide anion radical, hydrogen peroxide, and the hydroxyl radical. When released at a basal level, ROS have a regulatory effect on embryonic metabolism, embryonic development, and embryo implantation (3).Oxidative stress-induced damage to the embryo arises from excessively released ROS, and these radicals pass the cell membrane, causing structural changes in cellular molecules such as lipids, proteins, and nucleic acids, which, in turn, result in mitochondrial alterations, blockage of cellular development in the embryo, excessive consumption of ATP, and apoptosis (3-5). Eventually, embryonic development is inhibited and the implantation of the embryo is negatively affected (3).Against the oxidative stress and damages caused by ROS, in vivo antioxidant defense systems, which protect the embryo, exist in both the embryo and the female reproductive tract that it resides in. These antioxidant systems include, among others, glutathione, taurine, hypotaurine, ascorbate, pyruvate, vitamin E, glutathione peroxidase, superoxide dismutase, and catalase (3). Under in vitro conditions, as the embryo depends only on the antioxidant systems it is itself equipped with against oxidative stress, additional preventive measures need to be taken (6-8). However, the elimination of oxidative stress and oxidative stress factors from the embryo culture medium is both an inevitable and complex problem. The selection of antioxidants and antioxidant doses for the supplementation of culture media can be challenging, as excessive antioxidant doses in the medium may cause negative impacts (6,9).Previous research has shown that fatty acids incorporated into in vitro culture media exhibit positive effects on oocyte maturation, fertilization, and embryonic development (10,11). The feeding of ruminants on rations of high fat content produces positive impacts on reproductive performance and enables high blastocyst

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trans‐10, cis‐12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes

Jia

et al. 2013

Molecular Reproduction Devel

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The aim of this study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (t10c12 CLA) supplementation on oocyte maturation and embryo development in pigs. Compared with the control, supplementation of 50 µM t10c12 CLA to in vitro maturation (IVM) medium significantly increased the proportion of oocytes at the metaphase-II (MII) stage and subsequent parthenogenetic embryo development in terms of cleavage rate, blastocyst formation rate, and cell numbers in blastocysts. The t10c12 CLA-treated oocytes resumed meiotic maturation and progressed to the MII stage significantly faster than those of control. The expression of phosphorylated mitogen-activated protein kinase 3/1 (p-MAPK3/1) and cyclooxygenase-2 (COX2) in cumulus oocyte complexes (COCs) at 5, 10, and 22 hr of IVM were significantly increased in the t10c12 CLA-treatment group. The level of p-MAPK3/1 in t10c12 CLA-treated MII oocytes was also higher (P < 0.05) than that of control. Moreover, t10c12 CLA supplementation partially overcame the negative effects of U0126 on cumulus expansion and nuclear maturation, and completely recovered COX2 protein levels in the presence of U0126. Treatment of COCs with NS398 also significantly suppressed cumulus expansion and nuclear maturation, which was overcome by t10c12 CLA. Yet, this simulatory effect of t10c12 CLA was blocked in the presence of both U0126 and NS398. The t10c12 CLA treatment significantly reduced reactive oxygen species level and increased glutathione concentrations in MII oocyte. In conclusion, supplementation of t10c12 CLA during porcine oocyte maturation exerts its beneficial effects on nuclear and cytoplasmic maturation, which contributes to enhancing subsequent embryo development.

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Cryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA)

Cited by 82 publications

References 31 publications

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

Fat area and lipid droplet morphology of porcine oocytes during in vitro maturation with trans-10, cis-12 conjugated linoleic acid and forskolin

The effect of oleic and linoleic acids on in vitro bovineembryonic development and embryo quality

trans‐10, cis‐12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes

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