<i>trans</i>‐10, <i>cis</i>‐12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes

Jia, Baoyu; Wu, Guoquan; Fu, Xiangwei; Mo, Xianhong; Du, Min; Hou, Yunpeng; Zhu, Shi-En

doi:10.1002/mrd.22273

Cited by 16 publications

(7 citation statements)

References 68 publications

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“…We also selected a JAK inhibitor to disrupt JAK/STAT3 signaling because JAK is the key kinase responsible for the activation of STAT3. According to dose–response experiments done previously by our lab and others (Liu et al, ; Marei et al, ; Nguyen et al, ; Jia et al, ), we pretreated cultured bovine COCs with 10 µM U0126 or 5 µM JAK inhibitor, and then measured levels of phosphorylated MAPK (p‐MAPK3/1) or STAT3 (p‐STAT3). Adding U0126 or JAK inhibitor significantly reduced the relative levels of p‐MAPK3/1 or p‐STAT3 compared to total MAPK3/1 and STAT3 levels (Fig.…”

Section: Resultsmentioning

confidence: 99%

Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development

Yuan

et al. 2014

Molecular Reproduction Devel

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The present study was conducted to examine the effects of leukemia inhibitory factor (LIF) on bovine oocyte maturation and early embryo development in vitro. Results showed that LIF supplementation (25 ng/ml) enhanced nuclear maturation of intact cumulus-oocyte complexes (COCs) compared to the vehicle control. Similar results were observed in denuded oocytes, indicating that LIF directly influences oocyte development. LIF-treated oocytes showed a higher cortical-granule-migration rate and increased expression of CD9, a tetraspanin transmembrane protein essential for fertilization. After in vitro fertilization, oocytes receiving LIF supplementation exhibited a higher cleavage rate and yielded a significantly higher number of blastocysts. To further dissect the molecular mechanism underlying this LIF-induced bovine oocyte maturation phenotype, we examined the involvement of two signaling cascades, mitogen-activated protein kinases (MAPK3/1)- and the signal transducer and activator of transcription 3 (STAT3)-dependent pathways. Western blot results revealed that LIF phosphorylated MAPK3/1 and STAT3. Inhibition of MAPK3/1 activation with MEK inhibitor U0126 only partially blocked LIF-induced nuclear maturation, although it attenuated oocyte cytoplasmic maturation. Inhibition of JAK/STAT3 activation with a specific pharmacological inhibitor completely abolished the LIF-response in bovine oocyte. In summary, these data revealed a novel role for LIF in bovine oocyte maturation subsequent embryonic development.

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Section: Resultsmentioning

confidence: 99%

Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development

Yuan

et al. 2014

Molecular Reproduction Devel

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“…Various nutrients, metabolites, and substrates are transported through gap junction between cumulus cells and oocytes for sucessful maturation (Li et al, 2015). And several genes including PTX3, GJA1, and PTGS2 are involved with cumulus expansion and function of gap junction in mammalian oocytes (Zhang et al, 2005; Jia et al, 2014; Li et al, 2015). In present study, although treatment of 50 µM ALA enhanced cumulus expansion at 22 h after maturation, GJA1 mRNA in cumulus cells was downregulated at 44 h after maturation.…”

Section: Discussionmentioning

confidence: 99%

“…Reduction of GJA1 mRNA by ALA was similar with results of FA metabolism-related genes. Jia et al (2014) reported that trans-10, cis-12 conjugated linoleic acid (t19c12 CLA) promoted protein expression of cyclo-oxygenase 2 (COX2; also known as PTGS2) in porcine oocytes for 22 h after maturation, whereas it was not differed at 44 h. These findings lead to predict that ALA treatment during first 22 h of maturation would promote FAs metabolism and gap junction for transport and production of energy, however, these are supressed to maintain energy balance during 22 h after first maturarion period. Therefore, our futher plan include invastigation of cumulus expansion-related genes and cAMP levels during first 22 h in IVM.…”

Section: Discussionmentioning

confidence: 99%

Alpha-Linolenic Acid: It Contribute Regulation of Fertilization Capacity and Subsequent Development by Promoting of Cumulus Expansion during Maturation

Lee¹,

Hwangbo²,

Cheong³

et al. 2018

Dev. Reprod.

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The objective of this study was to evaluate the effects of alpha-linolenic acid (ALA) during in vitro maturation (IVM) on cumulus expansion, nuclear maturation, fertilization capacity and subsequent development in porcine oocytes. The oocytes were incubated with 0, 25, 50, and 100 μM ALA. Cumulus expansion was measured at 22 h, and gene expresison and nuclear maturation were analyzed at 44 h after maturation. Then, mature oocytes with ALA were inseminated, and fertilization parameters and embryo development were evaluated. In results, both of cumulus expansion and nuclear maturation were increased in 50 μM ALA groups compared to control groups (p<0.05). However, expression of gap junction protein alpha 1 (GJA1, cumulus expansion-related gene), delta-6 desaturase (FADS1, fatty acid metabolism-related gene), and delta-5 desaturase (FADS2) mRNA in cumulus cells were reduced by 50 μM ALA treatment (p<0.05). Cleavage rate was enhanced in 25 and 50 μM ALA groups (p<0.05), especially, treatment of 50 μM ALA promoted early embryo develop to 4 and 8 cell stages (p<0.05). However, blastocyst formation and number of cells in blastocyst were not differ in 25 and 50 μM ALA groups. Our findings show that ALA treatment during maturation could improve nuclear maturation, fertilization, and early embryo development through enhancing of cumulus expansion, however, fatty acid metabolism- and cumulus expansion-related genes were down-regulated. Therefore, addition of ALA during IVM of oocytes could improve fertilization and developmental competence, and further studies regarding with the mechanism of ALA metabolism are needed.

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“…The level of GSH in each blastocyst was measured with 10 μmol/L 4-chloromethyl-6.8-difluoro-7-hydroxycoumarin (Cell-Tracker Blue) with a filter at 370 nm excitation. The experimental procedure was same as the ROS measurement described above [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Zuogui Wan rescues the high-glucose-induced damaging effects on early embryo development

Bai

Feng

Zhu

et al. 2016

BMC Complement Altern Med

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BackgroundHigh concentration of glucose in culture medium affects the developmental process and the quality of the pre-implantation embryo. This study examined the effects of Zuogui Wan (ZGW) supplementation on early embryo development cultured in high-glucose medium.MethodsEmbryos were cultured in high-glucose medium with or without ZGW supplementation. Developmental rate and competence was evaluated by cleavage rate, blastocyst rate, and blastocyst total cell number, reactive oxygen species (ROS) level, glutathione (GSH) concentration, and metabolome were also measured to determine the effect of ZGW on embryo development at the cellular level.ResultsCompared with the vehicle group, supplementation of 0.01 % (v/v) ZGW to high-glucose medium significantly increased cleavage rate (80.1 ± 1.0 % vs 72.1 ± 1.3 %), blastocyst rate (50.5 ± 1.0 % vs 41.3 ± 1.7 %), and blastocyst total cell number (63.2 ± 2.2 vs 57.2 ± 1.6). ROS level was lower and GSH concentration in blastocysts was higher in ZGW-treated group. Metabolomic analysis found that the ratio of glucose to succinic acid and glucose to fumaric acid were lower in the ZGW-treated group .ConclusionsDevelopmental rates of zygotes in high-glucose culture medium were significantly lower than those in regular culture medium. ZGW supplementation significantly improved embryo development and quality in high-glucose medium. Supplementing ZGW in high-glucose medium also significantly increased total cell number and GSH concentration but decreased ROS level in blastocysts likely by modifying metabolic profile during embryo development. Together, these data suggest that supplementation of ZGW rescues high-glucose-induced detrimental effects on pre-implantation embryo development.

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trans‐10, cis‐12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes

Cited by 16 publications

References 68 publications

Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development

Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development

Alpha-Linolenic Acid: It Contribute Regulation of Fertilization Capacity and Subsequent Development by Promoting of Cumulus Expansion during Maturation

Zuogui Wan rescues the high-glucose-induced damaging effects on early embryo development

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