Cryosurgical technique: Assessment of the fundamental variables using human prostate cancer model systems

Klossner, Daniel P.; Robilotto, Anthony T.; Clarke, Dominic M.; VanBuskirk, Robert G.; Baust, John M.; Gage, Andrew A.

doi:10.1016/j.cryobiol.2007.07.003

Cited by 67 publications

(53 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was also observed in double heat exposure to 48°C, but not 45°C exposure. This suggests that in line with previous studies from Gage and Klossner, a repeat thermal exposure should cause a greater amount of damage and reduce the likelihood of disease recurrence in a clinical scenario [18,38].…”

Section: Discussionsupporting

confidence: 63%

See 1 more Smart Citation

Dual thermal ablation of pancreatic cancer cells as an improved combinatorial treatment strategy

Baumann¹,

Baust²,

Snyder³

et al. 2017

Liver Pancreat Sci

View full text Add to dashboard Cite

Pancreatic cancer (PaCa) is one of the leading causes of cancer related deaths in the world today. It is estimated that 95% of patients diagnosed with PaCa will die because of limited treatment options. As standard treatments have not yielded an improvement in patient outcome, alternative approaches, such as thermal ablation, may offer a new treatment path. We previously reported the lethal temperatures necessary for PaCa ablation for both freeze and heat based treatments. In this study, we investigated the response of PaCa cells in vitro to a combination treatment of heating and freezing, dual thermal ablation (DTA), in an effort to further improve ablation efficacy.PaCa cell lines, PANC-1 and BxPC-3, were subjected to heating (45 to 50°C), freezing (-10 to -20°C), or DTA exposure. Post-exposure viability was assessed over a 7-day recovery interval. Modes of cell death were analyzed using fluorescence microscopy and flow cytometry.Our results indicated that in comparison to single thermal modalities, DTA resulted in greater cell destruction at a more rapid rate. Specifically, -15°C had a moderate impact on day one cell viability (PANC-1=80%; BxPC-3=21%) and 50°C (PANC-1=74%; BxPC-3=18%) caused a slow decline in cell number post-treatment. The combination of these treatments resulted in an increased cell death one-day post-treatment (PANC-1=28%; BxPC-3=5%) and achieved complete cell destruction within three days. Overall, these data suggest that a combination of heat and freeze ablation, DTA, may provide for an improved treatment strategy for PaCa.

show abstract

Section: Discussionsupporting

confidence: 63%

“…Previous reports have demonstrated that repeated exposure of cancer cells to thermal treatment results in increased cell death [18,38]. As such, thermal ablation is typically applied as a monotherapy in a repeat exposure protocol.…”

Section: Impact Of Dual Thermal Ablationmentioning

confidence: 99%

Dual thermal ablation of pancreatic cancer cells as an improved combinatorial treatment strategy

Baumann¹,

Baust²,

Snyder³

et al. 2017

Liver Pancreat Sci

View full text Add to dashboard Cite

show abstract

“…First, passive thawing following freezing yielded greater cell death than active thawing. This observation is consistent with clinical comparison of cell viability from passive and active thawing samples [30]. Secondly, greater amount of cells survived when higher thawing temperatures were employed.…”

Section: Thawing Temperaturesupporting

confidence: 86%

On the study of the freeze–thaw thermal process of a biological system

Chua

Chou

2009

Applied Thermal Engineering

View full text Add to dashboard Cite

“…Thus, a passive thaw, using body heat for warming, is recommended for the first cycle while an active thaw may be appropriate during the second cycle. Though the duration of treatment to achieve irreversible tissue damage is not clear, freezing tissue between 5-10 min seems to adequately cause cell death [15]. Durable and similar outcomes have been seen with both laparoscopic and percutaneous approaches [16].…”

Section: Ablative Techniquesmentioning

confidence: 93%

Ablative Therapies for the Treatment of Small Renal Masses: a Review of Different Modalities and Outcomes

2016

View full text Add to dashboard Cite

The widespread utilization of abdominal imaging has led to an increase in incidentally detected small renal masses. Although partial nephrectomy is still considered the gold standard treatment for these masses, there are risks associated with surgical excision, potentially limiting treatment for older patients with multiple comorbidities. A variety of ablative techniques have developed over the past several decades, altering the management of small renal masses. It is likely that improvements in technology will only broaden the applications of ablative therapy. This article provides an update on the various ablative techniques and outcomes.

show abstract

Cryosurgical technique: Assessment of the fundamental variables using human prostate cancer model systems

Cited by 67 publications

References 29 publications

Dual thermal ablation of pancreatic cancer cells as an improved combinatorial treatment strategy

Dual thermal ablation of pancreatic cancer cells as an improved combinatorial treatment strategy

On the study of the freeze–thaw thermal process of a biological system

Ablative Therapies for the Treatment of Small Renal Masses: a Review of Different Modalities and Outcomes

Contact Info

Product

Resources

About