Cryoprotective effect of sericin supplementation in freezing and thawing media on the outcome of cryopreservation in human sperm

Aghaz, Faranak; Khazaei, Mozafar; Vaisi‐Raygani, Asad; Bakhtiyari, Mitra

doi:10.1080/13685538.2018.1529156

Cited by 22 publications

(10 citation statements)

References 42 publications

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“…This might be related to the higher lipid peroxidation in this group. Even though some studies showed that radical scavenging activity was dose‐dependent increasing with an increase in sericin concentration (Aghaz et al., 2018; Wu et al., 2007), in contrast the finding in our study was disputation. Our results are consistent with those of Terada, Nishimura, Sasaki, Yamada, and Miki (2002), in that 1% sericin supplementation had a negative effect on cell lines in mammals after storage.…”

Section: Discussioncontrasting

confidence: 99%

Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender

Ratchamak

Ratsiri

Kheawkanha

et al. 2020

Animal Science Journal

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Cryopreservation of boar semen is a technique used to extend the shelf life over that of cooled semen, which is useful to preserve genetic material for long periods (Qian, Yu, & Zhou, 2016). Artificial insemination (AI) was the first studied in 1957 (Hess, Teague, Ludwick, & Martig, 1957) and swine industry has been applying this biotechnology for several decades (Rath et al., 2009). On the other hand, this procedure does not exceed 1% of all the swine industry farm worldwide (Yeste, 2015). Frozen semen is commonly used in cattle due to the quality of semen after freezing and the satisfactory conception rate. However, the quality of boar semen after freezing has not been satisfactory. It results in a low conception rate and litter size that is not high enough to meet the requirements of the manufacturer. The issue occurs because of the low semen quality and low survival rate of spermatozoa after the freezing process (Eriksson, Petersson, & Rodriguez-Martinez, 2002). It was found that boar semen are sensitive to exposure to temperatures below 12°C, which induces destabilizing sperm membrane changes called "cold shock" as expressed by decreased sperm motility that appears to be the critical temperature to spermatozoa.

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Section: Discussioncontrasting

confidence: 99%

Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender

Ratchamak

Ratsiri

Kheawkanha

et al. 2020

Animal Science Journal

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“…This might be related to the higher lipid peroxidation in this group. Even though some studies showed that the scavenging activity of ROS was dose-dependent increasing with an increase in sericin concentration (Wu et al 2007;Aghaz et al 2018), however the best quality of post-thaw semen in our study was not observed at the highest sericin supplementation (1.0%). The higher concentration of antioxidants has been reported as toxic to sperm.…”

Section: Effect Of Sericin Concentration On Post-thaw Semen Qualitycontrasting

confidence: 87%

“…Results of another study indicated that 0.25-0.5% sericin supplementation improved the frozen-thawed buffalo bull semen quality by preventing oxidative stress (Kumar et al 2015). Also, the addition of 2.5-5% sericin to a freezing and thawing medium successfully increased the total motility and viability in human sperm (Aghaz et al 2018). This difference in concentration levels between studies might be due to the nature of extender, different species, and cryopreservation protocols.…”

Section: Effect Of Sericin Concentration On Post-thaw Semen Qualitymentioning

confidence: 99%

“…When in vitro-produced bovine embryos were frozen conventionally in the freezing medium supplemented with various concentrations (0.1%, 0.5%, and 1.0%). In sperm cells, sericin supplementation to an extender improved the quality of human, buffalo bull, goat, and boar frozen-thawed semen by protecting sperm from oxidative stress (Kumar et al 2015;Aghaz et al 2018;Reddy et al 2018;Ratchamak et al 2020). However, information about the use of sericin in the cryopreservation of dairy bull semen has never been reported.…”

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confidence: 99%

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Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

Yangnam¹,

Chapanya²,

Vongpralub³

et al. 2021

CZECH J ANIM SCI

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Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde – MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

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“…Antioxidant supplementation can potentially improve sperm cryopreservation outcomes. The addition of 5% sericin to freezing and thawing media increased total motility and viability and decreased DNA fragmentation relative to media without sericin [79]. Melatonin may exert its cryoprotective effects on spermatozoa by counteracting intracellular ROS and thereby reducing MDA generation, leading to an increase in the post-thaw viability and motility of cryopreserved human spermatozoa [68] and reducing oxidative damage by upregulating heat shock protein 90 (HSP-90) expression [80].…”

Section: Gpx4mentioning

confidence: 99%

Protective Effects of Curcumin on the Outcome of Cryopreservation in Human Sperm

et al. 2021

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Cryopreservation causes decreased sperm fertility potential due to reactive oxygen species (ROS) production and physical-chemical damage, resulting in reduced sperm viability and motility. The addition of antioxidants to freezing media could protect sperm from cryo-damage, counteracting the harmful effects of ROS. The aim of this study was to assess the effects of curcumin supplementation in freezing medium on preventing cryo-damage in human semen. Semen samples collected from fertile men were cryopreserved in freezing medium supplemented with different concentrations of curcumin (2.5, 5, 10, and 20 μM). After freezing-thawing, sperm parameters, DNA fragmentation, intracellular ROS, and glutathione peroxidase 4 (GPX4) gene expression were evaluated. Supplementation with 20 μM curcumin in freezing medium caused increases in progressive and nonprogressive motility and significant reductions in intracellular ROS and DNA fragmentation in frozen-thawed sperm cells. Following cryopreservation, GPX4 mRNA expression was significantly upregulated in thawed semen supplemented with 20 μM curcumin compared to the control. The results showed that curcumin supplementation in freezing medium was protective against human sperm parameters and sperm DNA, counteracting oxidative damage induced by the freeze-thaw process.

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Cryoprotective effect of sericin supplementation in freezing and thawing media on the outcome of cryopreservation in human sperm

Cited by 22 publications

References 42 publications

Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender

Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender

Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

Protective Effects of Curcumin on the Outcome of Cryopreservation in Human Sperm

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