Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (<i>Bombyx mori</i>) in semen extender

Ratchamak, Ruthaiporn; Ratsiri, Thanaporn; Kheawkanha, Theerapat; Vongpralub, Thevin; Boonkum, Wuttigrai; Chankitisakul, Vibuntita

doi:10.1111/asj.13428

Cited by 12 publications

(13 citation statements)

References 46 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sperm motility : Total motility and progressive motility were determined using a computer-assisted semen analyzer, CASA (Hamilton Thorne Biosciences, version 12 TOX VIOS, Beverly, MA, USA), as described by Ratchamak et al [ 19 ]. A minimum of 300 sperm per sample were counted.…”

Section: Methodsmentioning

confidence: 99%

“…Sperm viability, acrosome integrity, and mitochondrial function : The percentage sperm viability, acrosome integrity, and mitochondrial potential were determined by fluorescent multiple staining using propidium iodide (Live/dead sperm viability kit L7011; Invitrogen, Waltham, MA, USA), fluorescein isothiocyanate-labeled peanut (Arachishypogaea), agglutinin (FITC-PNA), and 5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining, respectively, as previously described by Ratchamak et al [ 19 ]. Three hundred sperm were assessed immediately by a fluorescence microscope with a triple filter, showing a set of UV-2E/C (excitation 340 to 380 nm and emission 435 to 485 nm), B-25/C (excitation 465 to 495 nm and emission 515 to 555 nm) and G-2E/C (excitation 540 to 525 nm and emission 605 to 655 nm) at ×400 magnification.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of gelatin and oxytocin supplementation in a long-term semen extender on boar semen quality and fertility potential

Chankitisakul,

Tubtimtong,

Boonkum

et al. 2024

Anim Biosci

Self Cite

View full text Add to dashboard Cite

Objective: This study investigated the efficacy of different concentrations of gelatin supplementation in long-term semen extender on boar semen quality during storage for 10 days at 17°C. Additionally, oxytocin was added to stored semen to enhance fertility.Methods: In Experiment 1, boar semen was collected, diluted with gelatin at concentrations between 0% and 2.5% (w/v) and mixed with a semen extender. Then, it was kept in a refrigerator at 17°C and stored for 10 days. In Experiment 2, the sperm quality was examined after adding 0, 5, and 10 IU oxytocin per artificial insemination dose to the most effective semen extender from Experiment 1 and placing it in a refrigerator at 17°C for 10 days. In Experiment 3, the fertility potential in terms of non-return rate and litter size was determined using the most effective solid-stored semen supplemented with oxytocin.Results: The results indicated that sperm quality decreased with increasing storage time (p<0.05). The sperm quality in terms of total motility, progressive motility, and viable sperm with intact acrosomes and high mitochondrial potential was the highest with 1.5% gelatin supplementation (p<0.001) on all days of storage. Treatment with oxytocin did not affect sperm quality (p>0.05). The non-return rate and litter size after insemination with semen supplemented with 1.5% gelatin and 10 IU of oxytocin after 8 to 10 days of storage were comparable to those of the control group (p>0.05).Conclusion: A semen extender as a solid medium supplemented with 1.5% gelatin successfully preserved boar semen for a long storage duration. Treatment with oxytocin did not affect sperm quality. In addition, the fertility capacity using 1.5% gelatin with 10 IU oxytocin and stored for 8 to 10 days was acceptable and comparable to that of short-term storage.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of gelatin and oxytocin supplementation in a long-term semen extender on boar semen quality and fertility potential

Chankitisakul,

Tubtimtong,

Boonkum

et al. 2024

Anim Biosci

Self Cite

View full text Add to dashboard Cite

show abstract

“…MDA concentration, an index of lipid peroxidation in the blood samples, was measured using the thiobarbituric acid (TBA) reaction as performed following the instructions provided by Ratchamak et al ( 30 ). Blood samples were added to 0.25 ml of ferrous sulfate (0.2 mM) and 0.25 mL of ascorbic acid (1 mM), and they were then incubated for 60 min in a 37°C water bath.…”

Section: Methodsmentioning

confidence: 99%

The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreeds Under Heat Stress Conditions

et al. 2022

View full text Add to dashboard Cite

The effect of heat stress with melatonin treatment on the superovulatory responses and embryo characteristics in Thai-Holstein crossbreeds under heat stress conditions was examined. Six non-lactating cows (replication = 4; n = 24) were assigned to one of 2 treatments in double cross-over design. All cows were superstimulated with traditional treatment. Melatonin group (n = 12): cows received intramuscularly injection 18 mg/50 kg. simultaneously with GnRH injection, while those in the control group (n = 12) received none. Bloods samples were taken to determine lipid peroxidation (MDA) and the activity of the antioxidant enzymes (superoxide dismutase; SOD). The experiment was conducted from April to September, which determined severe heat stress (the mean temperature-humidity index above 77). The results revealed that numbers of large follicles and corpora lutea were higher in the melatonin group than in the control group (p < 0.01). Numbers of recovered ova/embryos, fertilized ova, and transferable embryos were higher in the melatonin group (p < 0.01); meanwhile, more degenerated embryos were found in the control group (p < 0.01). Increased activity of the antioxidant enzymes SOD after melatonin administration decreased MDA levels (p < 0.05). In summary, melatonin administration benefited the ovarian response and embryo quality in superstimulated Thai-Holstein crossbreed affected by heat stress.

show abstract

“…Then, the mitochondria were labeled using 2 µL of JC-1 and incubated at 37 • C for 10 min. Finally, the acrosomes were labeled using 2 µL of FITC-PNA and incubated at 37 • C for 15 min in the dark [26]. Under a 400× magnification fluorescence microscope, 200 sperm were assessed.…”

Section: Assessment Of Mitochondrial Membrane Potentialmentioning

confidence: 99%

Palm Kernel Meal Protein Hydrolysates Enhance Post-Thawed Boar Sperm Quality

Khophloiklang,

Chanapiwat,

Aunpad

et al. 2023

Animals

View full text Add to dashboard Cite

Boar sperm is sensitive to particular conditions during cryopreservation, resulting in an extreme reduction in fertilizing ability due to damage to the sperm membranes. PKMPH contains bioactive peptides that have antioxidant and antimicrobial activities. There is no information on the use of palm-kernel-meal-derived bioactive peptides for boar semen cryopreservation. This study aimed to examine the effects of bioactive peptides from PKMPH on post-thawed boar sperm quality. Boar semen ejaculates (n = 17) were collected and divided into six equal aliquots based on PKMPH concentrations (0, 1.25, 2.5, 5, 10, and 15 µg/mL) in a freezing extender. Semen samples were processed and cryopreserved using the liquid nitrogen vapor method. Thereafter, the frozen semen samples were thawed at 50 °C for 12 s and evaluated for sperm motility using a computer-assisted sperm analyzer and for sperm viability, acrosome integrity, mitochondrial function, and lipid peroxidation by measuring the level of malondialdehyde (MDA). The results demonstrate that the supplementation of PKMPH with 2.5 µg/mL afforded superior post-thawed sperm qualities, such as increased total motility, viability, acrosome integrity, and mitochondrial function by 10.7%, 12.3%, 18.3%, and 12.7%, respectively, when compared to the control group. PKMPH at a concentration of 2.5 µg/mL showed the lowest level of MDA (40.6 ± 2.0 µMol/L) compared to the other groups. In conclusion, adding PKMPH peptides at 2.5 µg/mL to the freezing extender reduced the oxidative damage associated with cryopreservation and resulted in higher post-thawed sperm quality.

show abstract

Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender

Cited by 12 publications

References 46 publications

Effects of gelatin and oxytocin supplementation in a long-term semen extender on boar semen quality and fertility potential

Effects of gelatin and oxytocin supplementation in a long-term semen extender on boar semen quality and fertility potential

The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreeds Under Heat Stress Conditions

Palm Kernel Meal Protein Hydrolysates Enhance Post-Thawed Boar Sperm Quality

Contact Info

Product

Resources

About