Cryoprotective effect of antifreeze polyamino-acid (Carboxylated Poly-l-Lysine) on bovine sperm: A technical note

Fujikawa, T.; Imamura, S.; Tokumaru, Motoyuki; Ando, Takaaki; Gen, Yuki; Hyon, Suong‐Hyu; Kubota, Chikara

doi:10.1016/j.cryobiol.2018.02.009

Cited by 16 publications

(15 citation statements)

References 13 publications

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“…It has previously been confirmed that partial replacement of glycerol with CPLL protects the bovine sperm through reducing the membrane damage. 16 A recent study reported improvement in postthaw quality and fertility of buffalo sperm with 0.75% CPLL and 5% glycerol. 17 It is relevant to mention that CPLL possesses AFP-like properties that might have added protection to the sperm cells as has been reported using AFPs for buffalo sperm.…”

Section: Fig 6 Effect Of Different Concentrationsmentioning

confidence: 99%

“…39 Previous studies have reported its use as a cryoprotectant for cell [12][13][14] and oocyte 15 and sperm. 16 A recent study on Nili-Ravi buffalo sperm investigated two levels of glycerol (7% and 5%) with 0.25%-1% levels of CPLL for cryopreservation of Nili Ravi buffalo sperm. 17 This study is a step further to find some positive synergism between glycerol and CPLL at some lower level of glycerol, with a corresponding increased level of CPLL for buffalo bull sperm cryopreservation.…”

Section: Fig 4 Effect Of Different Concentrationsmentioning

confidence: 99%

“…Combinations of 0% to 1% glycerol with 7% and 6% CPLL did not show any beneficial effect on the aforementioned parameters. It is suggested that CPLL is a nonpermeating cryoprotectant and might not have been dehydrated adequately for cryopreservation, 16 while, glycerol, being a permeating cryoprotectant, might have induced rapid dehydration to protect cells from freezing. It has previously been confirmed that partial replacement of glycerol with CPLL protects the bovine sperm through reducing the membrane damage.…”

Section: Fig 6 Effect Of Different Concentrationsmentioning

confidence: 99%

“…12 The CPPL has been found to improve the quality and fertility of cryopreserved Japanese black bull semen. 16 A recent study investigated CPLL (0.25%-1%) with 5% and 7% glycerol levels for cryosurvivability of Nili-Ravi buffalo bull sperm. 17 However, we hypothesized that positive synergism might exist at some lower level of glycerol with a corresponding increased level of CPLL.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen

Akhter

Awan

Arshad

et al. 2020

Biopreservation and Biobanking

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Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezability of buffalo semen. The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergism toward cryosurvivability of Nili-Ravi buffalo sperm. Semen collected from four Nili-Ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based extenders, Control: (0% CPLL +7% Glycerol); E1: (1% CPLL +6% Glycerol); E2: (2% CPLL +5% Glycerol); E3: (3% CPLL +4% Glycerol); E4: (4% CPLL +3% Glycerol); E5: (5% CPLL +2% Glycerol); E6: (6% CPLL +1% Glycerol), and E7: (7% CPLL +0% Glycerol), and cryopreserved using a programmable cell freezer. Percentages of post-thaw sperm motility, plasma membrane integrity, and acrosomal integrity were found to be higher (p < 0.05) in extenders E1, E2, E3, E4, and E5 compared to E6 and the control. Sperm livability (%; live/dead ratio) and viability (%; live sperm with intact acrosome) were higher (p < 0.05) in extender E4 compared to all the other extenders. Sperm DNA integrity was higher (p < 0.05) in extender E2, E3, E4, and E5 compared to control, E1, and E6 extenders. Sperm lipid peroxidation levels were lower (p < 0.05) in E3, E4, E5, and E6 compared to control, E1, E2, and E7 extenders. Total antioxidant capacity of seminal plasma was higher (p < 0.05) in extenders E5, E6, and E7 than control, E1, E2, E3, and E4 extenders. It is concluded that synergism between CPLL and glycerol (4% CPPL and 3% Glycerol) seems to improve the freezability of Nili-Ravi buffalo semen by reducing oxidative stress.

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Section: Fig 6 Effect Of Different Concentrationsmentioning

confidence: 99%

Section: Fig 4 Effect Of Different Concentrationsmentioning

confidence: 99%

Section: Fig 6 Effect Of Different Concentrationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen

Akhter

Awan

Arshad

et al. 2020

Biopreservation and Biobanking

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show abstract

“…For this reason, the combination of glycerol with non-permeating cryoprotectants (egg yolk, fructose, sucrose or trehalose) seems to be the best alternative to reduce glycerol concentration and its side effects in red deer [126], buffalo [127], bull [128], buck [129] and ram sperm [130]. Recently, a novel cryoprotective agent, carboxylated poly-L-lysine, has been used to reduce glycerol concentration in the freezing medium, enhancing in vivo fertility of cryopreserved buffalo and bull sperm [131,132]. Regarding non-permeating cryoprotectants, it has been reported that egg yolk also alters the proteome of ram sperm before cryopreservation [40].…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

138

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Protective effects of trehalose on frozen-thawed ovarian granulosa cells of cattle

Zheng

Liu

et al. 2019

Animal Reproduction Science

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Cryoprotective effect of antifreeze polyamino-acid (Carboxylated Poly-l-Lysine) on bovine sperm: A technical note

Cited by 16 publications

References 13 publications

Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen

Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Protective effects of trehalose on frozen-thawed ovarian granulosa cells of cattle

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