Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (<i>Bubalus bubalis</i>) Semen

Akhter, Shamim; Awan, Muhammad Amjad; Arshad, Javeria; Rakha, Bushra Allah; Ansari, Muhammad Tayyab; Iqbal, Sajid

doi:10.1089/bio.2019.0120

Cited by 8 publications

(13 citation statements)

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“…A total of 100 spermatozoa were assessed and differentiated into four categories as acrosome intact and live (AIL), acrosome lost and live (ALL), acrosome intact and dead (AID) and acrosome lost and dead (ALD) (Nasreen et al., 2020). The following formulae were used for calculation of sperm viability and livability (Akhter et al., 2020).

Sperm Viability (%) = \frac{AIL}{ALL + AID + ALD} \times 100

Sperm Livability (Live / Dead Ratio) (%) = \frac{AIL + ALL}{AID + ALD} \times 100

…”

Section: Methodsmentioning

confidence: 99%

“…A total of 200 spermatozoa form different microscopic fields were evaluated under epifluorescence microscope (Labomed LX400) equipped with excitation filter (480) and barrier filter (550 nm) filter at 400×. Spermatozoa having damaged DNA emitted yellow to red fluorescence, while spermatozoa having intact DNA emitted green fluorescence (Akhter et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

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Sperm binding to hyaluronan is an excellent predictor of Nili‐Ravi buffalo bull fertility

et al. 2021

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This study reports the first evaluation of sperm hyaluronan binding assay (HBA) for predicting the fertility of Nili‐Ravi buffalo bulls in relation to standard parameters of sperm quality. Cryopreserved semen doses of low (n = 6), medium (n = 3) and high fertility (n = 8) bulls based on their respective return rates were used. Significantly, more spermatozoa bound to hyaluronan from the most fertile bulls (57.15% ± 1.44) compared with medium (42.46% ± 1.08) and low fertility bulls (29.70% ± 0.78). A strongly positive correlation (r = .824, p < .01) was found between HBA and fertility that predicts a 67.9% variability (r2 = .679, p < .01) in fertility. HBA was also strongly positively correlated with sperm viability (r = .679, p < .01) followed by their live/dead ratio (r = .637, p < .01), uncapacitated spermatozoa (r = .631, p < .01), normal apical ridge (r = .459, p < .01), motility (r = .434, p < .01), mature spermatozoa with low residual histones (r = .364, p < .01), high plasma membrane integrity (r = .316, p < .01) and nonfragmented DNA levels (r = .236, p < .05). It was negatively correlated with spermatozoa having reacted acrosome (r = −.654, p < .01). A fertility model built using a combination of sperm HBA and either sperm livability or viability predicts, respectively, 86.1% (r2 = .861, p < .01) and 85.9% (r2 = .859, p < .01) variability in buffalo bull fertility. In conclusion, sperm HBA may prove to be a single robust predictor of Nili‐Ravi buffalo bull fertility.

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Sperm Viability (%) = \frac{AIL}{ALL + AID + ALD} \times 100

Sperm Livability (Live / Dead Ratio) (%) = \frac{AIL + ALL}{AID + ALD} \times 100

…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Sperm binding to hyaluronan is an excellent predictor of Nili‐Ravi buffalo bull fertility

et al. 2021

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“…Cryoprotectant agents (CPAs) are used since their first discovery for reducing cryo-damages to sperm across the species (Akhter et al, 2020). Methanol, DMSO, sucrose and glycerol are the most commonly used CPAs for fish sperm.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of carboxylated poly‐l‐lysine for cryopreservation of Labeo rohita sperm

Bibi

Awan

Rakha

et al. 2023

Reprod Domestic Animals

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Carboxylated poly‐l‐lysine (CPLL) is an anti‐freeze agent having pronounced non‐permeating yet membrane stabilizing cryoprotective capabilities. The objective was to evaluate the CPLL supplementation in extender in terms of post‐thaw quality (sperm), total anti‐oxidant activity (milt) and fertilization potential of cryopreserved Labeo rohita sperm. For this purpose, male brood fish reared at a fish seed hatchery, Rawal Town Islamabad, Pakistan were captured from different rearing ponds and acclimatized in hatchery ponds for 6 h. The brooder was injected with Ovaprim (0.2 mL/kg), and milt was collected after 8 h in cooled sterilized falcon tubes, maintained at 4°C and evaluated for sperm motility. The milt collected from three brooders (n = 3) was diluted in extenders viz., modified Kurokura‐2 extender having 10% methanol (control); experimental extenders with CPLL supplementation at the rate of 0.5%, 1% and 1.5%. Diluted milt was filled in 0.5 mL straws, exposed to liquid nitrogen vapours and cryopreserved. Cryopreserved milt was thawed at 25°C and assessed for post‐thaw sperm quality. Sperm motility, motility duration, viability, total anti‐oxidant capacity and DNA integrity was significantly higher (p < 0.05) in the extender having 1.5% CPLL than control. To evaluate the fertilization rates, male and female brooders were injected with Ovaprim at 0.2 mL/Kg and 0.5 mL/Kg body weight respectively. Fresh eggs and milt were collected through abdominal stripping. Batches of 10 g of eggs from each female (n = 2) were fertilized with one straw, each from frozen sperm with KE + methanol (control), KE + methanol + 1.5% CPLL and 50 μL fresh milt (negative control). After 1.5 h of fertilization, eggs were collected from all jars and a total of 200 eggs were counted. The fertilized eggs appeared clear and transparent while unfertilized eggs looked opaque with disintegrated nuclei. Sperm fertilization rate (%) was higher (p < 0.05) in extender KE + methanol + 1.5% CPLL (78.7 ± 0.5) compared to control (KE + methanol) (52.0 ± 0.4) however, it was lower compared to that of negative control, the fresh milt (85.2 ± 0.6). In conclusion, supplementation of carboxylated poly‐l‐lysine (1.5%) to modified Kurokura‐2 extender having 10% methanol improves post‐thaw motility, motility duration, viability, DNA integrity, anti‐oxidant capacity (milt) and fertilizing ability of cryopreserved L. rohita sperm.

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“…Thiobarbituric acid (TBA) assay was used to determine the malondialdehyde (MDA) concentrations of spermatozoon. MDA profile is considered as indices of lipid peroxidation in spermatozoa as described in the previous studies (Akhtar et al, 2020; Garg et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant effect of Moringa olifera leaves extract in extender improves post‐thaw quality, kinematics, lipid peroxidation, total antioxidant capacity and fertility of water buffalo bull semen

Iqbal¹,

Naz²,

Bhutta³

et al. 2021

Andrologia

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Sperm cryopreservation is an important reproductive biotechnology, widely used to preserve the male fertility. It has made possible the successful implementation of reproductive techniques such as artificial insemination (A.I) and in vitro fertilization (IVF) to improve the rapid genetic potential of domestic animals worldwide (Bailey et al., 2003;Kumar et al., 2019;Medeiros et al., 2002). The spermatozoa experience several structural and functional alterations during the cryopreservation process, which ultimately compromise the sperm quality

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Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen

Cited by 8 publications

References 38 publications

Sperm binding to hyaluronan is an excellent predictor of Nili‐Ravi buffalo bull fertility

Sperm binding to hyaluronan is an excellent predictor of Nili‐Ravi buffalo bull fertility

Evaluation of carboxylated poly‐l‐lysine for cryopreservation of Labeo rohita sperm

Antioxidant effect of Moringa olifera leaves extract in extender improves post‐thaw quality, kinematics, lipid peroxidation, total antioxidant capacity and fertility of water buffalo bull semen

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