Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes

Yamaji, Yohei; Valdez, Delgado M.; Seki, Shinsuke; Yazawa, Ken-ichi; Urakawa, C; Jin, Bo; Kasai, Magosaburo; Kleinhans, F.W.; Edashige, Keisuke

doi:10.1016/j.cryobiol.2006.06.008

Cited by 34 publications

(24 citation statements)

References 21 publications

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“…The first one, an acid phosphatase tartrate-resistant, is expressed in zebrafish embryonic development (Thisse et al, 2001). Members of the aquaporins family play a role in the ovarian follicle maturation and egg hydration in fishes (Bobe et al, 2006, Fabra et al, 2005Yamaji et al, 2006), and AQP3 has been detected in zebrafish gastrula (Thisse and Thisse, 2004). NPC2, whose name recall a human disorder of cholesterol storage, the NiemannPick disease type C2, is a gene poorly described in the ovary, and involved in sperm maturation in the epididymus (Dacheux et al, 2006).…”

Section: Discussionmentioning

confidence: 98%

Genes expressed in Blue Fin Tuna (Thunnus thynnus) liver and gonads

Chini

Cattaneo

Rossi

et al. 2008

Gene

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Section: Discussionmentioning

confidence: 98%

Genes expressed in Blue Fin Tuna (Thunnus thynnus) liver and gonads

Chini

Cattaneo

Rossi

et al. 2008

Gene

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“…In most cases, this protein is expressed in epithelial cells or on the lumen surface. Using X. laevis oocytes, several studies showed that this protein carries glycerol and other substances such as ethylene glycol, propylene glycol and acetamide (Yamaji et al, 2006), reasons why it is considered a GLP. Beyond these observations, the permeability and hydraulic conductivity of the channel were evaluated, where it was found that AQP3 plays an important role in transporting ethylene glycol, propylene glycol, and glycerol during bovine embryo cryopreservation.…”

Section: Main Types and Functions Of Aquaporinsmentioning

confidence: 99%

Review Structure, function, and localization of aquaporins: their possible implications on gamete cryopreservation

Sales¹,

Lobo²,

Carvalho³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The discovery of water channels (aquaporins, AQPs) was a landmark event for the clarification of water transport through the plasma membrane. AQPs belong to a family of intrinsic membrane proteins that act as selective channels for water and for solutes such as glycerol and urea. AQPs were found in different tissues and organs, including male and female reproductive systems. In the swine female reproductive system, the AQPs were localized in the uterus, oviduct, and ovary, as well as in the granulosa cells from primordial follicles. Knowing the involvement of AQPs with the male and female germ cells, as well as their acknowledged role in transporting water through the plasma membrane, the research of these proteins in cryopreservation processes becomes essential. Thus, this review aims to describe the structure and function of AQPs in membranes, highlighting their role 6719 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 6718-6732 (2013) Overview and role of aquaporins on gamete cryopreservation in the reproductive system (male and female). We also discuss the involvement of AQPs in cryopreservation, focusing on the effect and importance of these proteins on the rates of vitrification protocols for preantral follicles present in the ovarian tissue of domestic mammals.

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“…AQP3 cRNA was synthesized as described elsewhere [8,9]. Briefly, AQP3 cDNA was cloned from rat kidney cDNA by polymerase chain reaction (PCR); the sense strand was 5'-CGGGATCCCATGGGTCGACAGAAGGAGTT-3', and the antisense strand was 5'-GCTCTAGAGGGTTTTATGGGGT-GTCC-3' (underlined sequences indicate inserted BamHI and XbaI sites, respectively).…”

Section: Preparation Of Aqp3 Crnamentioning

confidence: 99%

Developmental Ability of Vitrified Mouse Oocytes Expressing Water Channels

Yamaji

Seki

Matsukawa

et al. 2011

Journal of Reproduction and Development

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Abstract. Previously, we showed that the exogenous expression of aquaporin 3 (AQP3), an aquaglyceroporin, improved the tolerance of mouse oocytes to vitrification with a glycerol-based solution. In the present study, we examined conditions suitable for the expression of AQP3 and the ability of vitrified oocytes to develop in vitro and in vivo after fertilization. After only partial remove of cumulus cells, immature mouse oocytes (germinal vesicle stage) were injected with 5, 10 or 20 pg of AQP3 cRNA and cultured for 12 h for maturation. When matured oocytes were vitrified with a glycerol-based solution, 57-61% survived regardless of the amount of cRNA injected (5-20 pg). By contrast, no oocytes injected with water (control) survived. When the zona pellucida was removed from the vitrified oocytes and the oocytes were then fertilized in vitro and cultured, the proportions that were fertilized and developed into blastocysts were higher when the amount of cRNA injected was 5 pg than 10-20 pg. When 16 blastocysts were transferred to a pseudopregnant mouse, 5 developed to term, demonstrating that oocytes vitrified after injection of AQP3 cRNA retained the ability to develop to term. The water-permeability of cRNA-injected oocytes was higher than that of control oocytes from the maturing stage to the 1-cell zygote stage, whereas glycerol-permeability was higher only at metaphase II. This indicates that AQP3 was expressed for a relatively short period of time. These results suggest that the transient expression of water/cryoprotectant channels is effective for cryopreserving cells that have low membrane-permeability, such as mammalian oocytes. Key words: Aquaporin, Development, Membrane-permeability, Oocyte, Vitrification (J. Reprod. Dev. 57: [403][404][405][406][407][408] 2011) he permeability of the plasma membrane to water and cryoprotectants is one of the most important factors for successful cryopreservation of oocytes/embryos because it influences the formation of intracellular ice, the toxicity of cryoprotectants and osmotic swelling [1]. For cryopreservation of oocytes/embryos, vitrification has advantages over slow freezing in terms of the simplicity of the method and viability of the cells. As the solution used for vitrification contains a high concentration of cryoprotectants, it is much more toxic than the solution for slow-freezing. Therefore, the permeability of the plasma membrane would affect the survival of cryopreserved cells more in vitrification than in slow freezing. If the permeability is low, insufficient exposure to the cryoprotectants causes formation of intracellular ice. However, excessive exposure results in toxic effects of the cryoprotectants. Therefore, high permeability to water and cryoprotectants would be preferable for vitrification.We showed that a simple one-step method is effective for vitrification of mouse morulae [2] but that a two-step method with a pretreatment with a lower concentration of the cryoprotectant is favorable for vitrifying mouse embryos at early cleavage stages...

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Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes

Cited by 34 publications

References 21 publications

Genes expressed in Blue Fin Tuna (Thunnus thynnus) liver and gonads

Genes expressed in Blue Fin Tuna (Thunnus thynnus) liver and gonads

Review Structure, function, and localization of aquaporins: their possible implications on gamete cryopreservation

Developmental Ability of Vitrified Mouse Oocytes Expressing Water Channels

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