Cryopreservation of whole adipose tissue for future use in regenerative medicine

Effect of mild heat stress on the proliferative and differentiative ability of human mesenchymal stromal cells

“…All samples were obtained with written consent from the donors. MSCs from adipose tissue were isolated as described [10]. The tissue was washed with phosphate-buffered saline (PBS) and treated with 0.2% collagenase type IV in PBS for 20 min at 37 C. Collagenase activity was neutralized with 20 mL of fetal bovine serum (FBS) containing media and filtered through a sieve.…”

Cryopreservation can be used as an anti-aging strategy

“…MSCs isolated from fresh and frozen tissue were capable of differentiating along adipogenic, chondrogenic, osteogenic and neurogenic pathways, as confirmed by histology and real-time polymerase chain reaction (RT-PCR) analysis of tissue-specific messenger RNAs (mRNAs). No significant functional differences were observed between MSCs from frozen tissue as compared with fresh tissue [4,5]. Similar experiments have also been performed by preserving isolated cells rather than whole tissues.…”

“…Therefore, different methods of both cryopreservation and thawing (most commonly slow-and rapid-cooling methods) and type and concentration of cryoprotectant (phosphate buffer saline human albumin, dimethyl sulfoxide, ethylene glycol and sucrose) have been used to get the desired outcomes such as viability, proliferation and differentiation potential. Although previous reports in the literature are varied, the methodology that we used can successfully cryopreserve cells/tissues in a liquid nitrogen container and later thaw the cells/tissues for clinical use [4,5]. The experiments performed in our laboratory in this regard are very promising (Figure 1).…”

“…Therefore, other anti-aging strategies are required that are safe and effective and that do not compromise biological properties of cells. Recent studies have demonstrated no negative effect of cryopreservation on the functionality of stem and progenitor cells obtained from various sources [4,5].…”

Long-term frozen storage of stem cells: challenges and solutions

“…Methodology has been developed, which allows for economical, closed system processing that meets FDA requirements for minimal manipulation utilizing modified syringes. 40 Automated approaches to processing can be found with companies such as BioSafe, GDP Inc., Cytori, and American Cryostem. The automated approaches tend to be more expensive, requiring the purchase of machinery and/or expensive consumables.…”