Cryopreservation can be used as an anti-aging strategy

Choudhery, Mahmood S; Harris, David T.

doi:10.1016/j.jcyt.2014.08.012

Cited by 8 publications

(6 citation statements)

References 5 publications

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“…From this perspective, human PDLSCs or other dental stem cells, such as dental pulp stem cells and gingival-derived stem cells, may be obtained from discarded teeth during surgical procedures at various time points (e.g., in the case of tooth extraction due to impaction-related or orthodontic reasons during youth). Even for prolonged periods of time, cryopreservation of these young-aged cells under viable conditions demonstrates the potential to maintain their youthful bioactivities for future autologous applications, when needed by the donor for a specific medical condition [68]. Meanwhile, those cells offer potential allogenic applications as more work is being conducted to investigate the anti-inflammatory and immunomodulatory functions of MSCs [16,33,34].…”

Section: Discussionmentioning

confidence: 99%

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

et al. 2015

Acta Biomaterialia

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Section: Discussionmentioning

confidence: 99%

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

et al. 2015

Acta Biomaterialia

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“…Although the osteogenic potential of ability ADSCs is lower than BMSCs or DPSCs, the advantage on the availability of the tissue has made ADSCs still a good stem cell source. The different capacity of ADSCs might depend on patients and sources of fatty tissue . It is possible that ADSCs require longer induction periods or more supplement substances such as bone morphogenetics protein‐2 to facilitate bone cells generation …”

Section: Discussionmentioning

confidence: 99%

“…The different capacity of ADSCs might depend on patients and sources of fatty tissue. 8,38,39 It is possible that ADSCs require longer induction periods or more supplement substances such as bone morphogenetics protein-2 to facilitate bone cells generation. 40 In conclusion, the results in this study demonstrated that PCL/HAp scaffold supports BMSCs, DPSCs and ADSCs proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering

Chuenjitkuntaworn

Osathanon

Nowwarote

et al. 2015

J Biomedical Materials Res

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Major drawbacks of using an autograft are the possibilities of insufficient bony source and patient's morbidity after operation. Bone tissue engineering technology, therefore, has been applied for repairing bony defects. Previous study showed that a novel fabricated 3D-Polycaprolactone/Hydroxyapatite (PCL/HAp) scaffold possessed a good biocompatibility for bone cells. This study aimed to determine the ability of PCL/HAp for supporting cell growth, gene expression, and osteogenic differentiation in three types of mesenchymal stem cells, including bone marrow-derived mesenchymal stem cells (BMSCs), dental pulp stem cells (DPSCs), and adiposed-derived mesenchymal stem cells (ADSCs). These were assessed by cell viability assay (MTT), reverse-transcription polymerase chain reaction (RT-PCR) analysis, alkaline phosphatase activity, and osteogenic differentiation by alizarin red-S staining. The results showed that PCL/HAp scaffold could support growth of all three types of mesenchymal stem cells. In addition, DPSCs with PCL/HAp showed the highest level of calcium deposition compared to other groups. In conclusion, DPSCs exhibited a better compatibility with these scaffolds compared to BMSCs and ADSCs. However, the PCL/HAp could be a good candidate scaffold for all tested mesenchymal stem cells in bone tissue engineering.

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“…However, with this growing interest in possible cell therapeutics using AT-MSCs [5], many fundamental questions arise as to the logistics of a clinical application of these cells. One issue that arises in pursuit of this type of application is the method of conservation and maintenance of differentiation potential of AT-MSCs after undergoing a period of time.…”

Section: Introductionmentioning

confidence: 99%

Study on viability and chondrogenic differentiation of cryopreserved adipose tissue-derived mesenchymal stromal cells for future use in regenerative medicine

et al. 2015

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Adipose-derived mesenchymal stromal cells are promising as a regenerative therapy tool for defective tissues in mesenchymal lineage, including fat, bone, cartilage, and blood vessels. In potential future clinical applications, adipose-derived stem cell cryopreservation is an essential fundamental technology. The aim of this study is to define an adequate protocol for the cryopreservation of adipose-derived mesenchymal stromal cells, by comparing various protocols so as to determine the effects of cryopreservation on viability and chondrogenic differentiation potential of adipose-derived stem cells upon freeze-thawing of AT-MSCs colonies cryopreserved with standard and modified protocols, using flow cytometry and confocal microscopy. The study concludes that adipose-derived mesenchymal stromal cells could be long-term cryopreserved without any loss of their proliferative or differentiation potential.

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Cryopreservation can be used as an anti-aging strategy

Cited by 8 publications

References 5 publications

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering

Study on viability and chondrogenic differentiation of cryopreserved adipose tissue-derived mesenchymal stromal cells for future use in regenerative medicine

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