Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols

Courbière, Blandine; Odagescu, Valentina; Baudot, Anne; Massardier, Jérôme; Mazoyer, Claire; Salle, Bruno; Lornage, Jacqueline

doi:10.1016/j.fertnstert.2006.05.019

Cited by 73 publications

(77 citation statements)

References 33 publications

(48 reference statements)

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“…Lower concentrations of cryoprotectant are required in freezing media for slow cooling, which reduces the risk of toxic and osmotic damage to cells, but this is insufficient to prevent ice crystal formation jeopardizing cell survival during the freezing procedures (Vajta 2006). Vitrification, though still regarded as a relatively novel freezing method, has recently been reported as an effective alternative method for the cryopreservation of ovarian tissue in various species, including mouse (Wang et al 2009), rat (Deng et al 2009), pig (Gandolfi et al 2006), goat (Santos et al 2007), sheep (Al-aghbari & Menino 2002, Courbiere et al 2006, monkey (Yeoman et al 2005), and human (Keros et al 2009, Zhou et al 2010. It combines a high cooling rate with a high concentration of cryoprotectant in the vitrification media, which rapidly dehydrates the cells and prevents water from precipitating as ice (Vajta 2006).…”

Section: Introductionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary follicle with a diameter around 100-130 mm) were mechanically isolated from vitrified-warmed and fresh adult mouse ovarian tissues and cultured for 12 days followed by an ovulation induction protocol at the end of this period to initiate oocyte maturation. Oocytes were then released from these follicles, fertilized in vitro, and cultured to the blastocyst stage and vitrified. After storage in liquid nitrogen for 2 weeks, groups of vitrified blastocysts were warmed and transferred into pseudo-pregnant recipient females. Although most of the isolated mouse pre-antral follicles from fresh (79.4%) and vitrified (75.0%) ovarian tissues survived the 12-day in vitro culture period, significantly fewer mature oocytes developed from vitrifiedwarmed pre-antral follicles than from the fresh controls (62.2 vs 86.4%, P!0.05). No difference was observed in embryo cleavage rates between these two groups, but the proportion of embryos that developed into blastocysts in the vitrification group was only half that of the controls (24.2 vs 47.2%, P!0.05). Nevertheless, live births of healthy normal pups were achieved after transfer of vitrified blastocysts derived from both experimental groups. This study shows that successful production of healthy offspring using an in vitro follicle culture system is feasible, and suggests that this procedure could be used in cancer patients who wish to preserve their fertility using ovarian tissue cryopreservation.

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Section: Introductionmentioning

confidence: 99%

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Wang¹,

Catt²,

Pangestu³

et al. 2011

Reproduction

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“…As a consequence, we have chosen to evaluate oocyte morphology considering only the chromatin material aspect. Because the trypan blue test provides a means to estimate the follicular viability after cryopreservation, it has been used as a tool to assess the efficiency of the cryopreservation procedure [16][17][18]25]. Besides, hormone production in culture is a sign of follicular growth in culture and thus an evaluation marker of viability, as hormone can be secreted only from secondary stages onwards, therefore the hormonal activity of thawed and cultured tissue is a criterion for the effectiveness of the cryopreservation protocol [40].…”

Section: Discussionmentioning

confidence: 99%

“…With the aim of increasing cryoprotectant penetration of the medullar compartment and avoiding intravascular ice formation, cryoprotective medium was perfused through the ovarian artery at a constant pressure [15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Zhang

Ruan

et al. 2012

J Assist Reprod Genet

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Purpose The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation Methods Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured. Results When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols. Conclusion Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Zhang

Yan

Cao

et al. 2011

J Assist Reprod Genet

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Purpose The aim of this study was to detect the effects of different cooling rates and different ice-seeding temperatures on the cryopreservation of whole ovaries. Methods Cow whole ovaries were slowly frozen using different protocols with different cooling rates and different ice-seeding temperatures. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the follicular densities of grafts were measured. Results Protocol IIb was most effective protocol. Protocol Ib was more effective than protocol Ia and protocol Ic, and protocol IIIb was more effective than protocol IIIa and protocol IIIc. Conclusions Protocol IIb (the cooling rate was 0.2°C/min, and the ice-seeding temperature was −5°C) was appropriate for slow freezing of cow whole ovaries.

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Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols

Cited by 73 publications

References 33 publications

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

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