Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Zhang, Jianmin; Yan, Sheng; Cao, Yongzhi; Wang, Hongyan; Chen, Zi‐Jiang

doi:10.1007/s10815-011-9557-1

Cited by 18 publications

(11 citation statements)

References 29 publications

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“…A comparable perfusion time (40 min) required for optimal cryopreservation of bovine ovaries with DMSO was reported by Zhang et al [ 33 , 34 ]. While the majority of our data was obtained using bovine ovaries as a model system, our final experiments with human ovaries show that our bovine data actually can be extrapolated to the human situation.…”

Section: Discussionmentioning

confidence: 52%

Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant

Westphal

Gerritse

Braat

et al. 2017

J Assist Reprod Genet

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PurposeThis study aims to determine the optimal cryopreservation protocol for whole ovaries intended for preservation of fertility in women.MethodsWe investigated the optimal cryopreservation procedure for whole ovaries in a bovine model. The following parameters were investigated to determine their effect on ovarian tissue viability: type of cryoprotectant, administration route of the cryoprotectant (perfusion and/or submersion), and the maximum tolerable interval between death of the animal and start of the cryopreservation process. The resulting optimal cryopreservation procedure for bovine ovaries was subsequently tested on human ovaries. In vitro glucose uptake, histology, and immunohistochemistry were used to assess the integrity of the ovarian tissue.ResultsStarting the cryopreservation procedure (including perfusion with and submersion in DMSO) within 10–15 min after death of the animal proved critical, resulting in a 90–100% protection level against cryodamage. When cryopreserving human ovaries using the same protocol, over 95% protection against cryodamage was observed on all tissue levels. In addition, no apparent morphological damage to either the follicles or the vascular endothelium was observed.ConclusionOur findings suggest that using the optimized protocol presented in this paper allows good cryopreservation of whole human ovaries and represents an important step in considering whole ovary autotransplantation for clinically applied fertility preservation.

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Section: Discussionmentioning

confidence: 52%

Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant

Westphal

Gerritse

Braat

et al. 2017

J Assist Reprod Genet

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“…During slow freezing processes, cells undergo dehydration to remove intracellular water and to prevent intracellular ice formation (ZHANG et al 2011). To enhance dehydration, the cells are subjected to an ice-seeding process, which induces extracellular ice crystallization.…”

Section: Discussionmentioning

confidence: 99%

Cryoconservation of Chicken Blastodermal Cells: Effects of Slow Freezing, Vitrification, Cryoprotectant Type and Thawing Method During <I>In Vitro</I> Processing

Sawicka

Chojnacka-Puchta

Brzezińska

et al. 2015

folia biol (krakow)

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SAWICKA D., CHOJNACKA-PUCHTA L., BRZEZINSKA J., LAKOTA P., BEDNARCZYK M. 2015. Cryoconservation of chicken blastodermal cells: Effects of slow freezing, vitrification, cryoprotectant type and thawing method during in vitro processing. Folia Biologica (Kraków) 63: 129-134. Cryoconservation of blastodermal cells (BCs) can preserve genetic material for the future reconstruction of poultry breeds. The aim of our study was to compare the effects of three slow freezing programs and vitrification, different cryoprotectants (5% DMSO, 10% DMSO, or multi-component cryoprotectant (MC) and two thawing methods on the viability of chicken BCs. Significant differences in the survival of slowly frozen BCs using program 3 (2°C/min. to 0.4°C/min.) compared with programs 1 (1°C/min. to 0.3°C/min.) and 2 (4°C/min. to 0.3°C/min.) were observed. The percentage of live BCs was significantly higher after slow freezing in the presence of the MC compared with DMSO. The thawing method did not have a significant effect on the percentage of live BCs. We also observed significant differences in the survival rate of BCs after vitrification (81%) and slow freezing in the presence of 10% DMSO using program 3 (60%). The highest percentage of viable BCs was achieved by slow freezing with the MC using program 2 and thawing with method 1 (94%). The most unfavorable combination for BCs survival was slow freezing in 5% DMSO using program 3 and thawing with method 2 (58.3%). This is the first study to apply MC to the slow freezing of BCs. We also showed successful BCs vitrification.

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“…In sheep studies, most follicular population was lost because of vascular complications after transplantation with vascular anastomosis [16,17,23], although a successful pregnancy and an ovarian function of 6 years were reported after whole ovary cryopreservation and autotransplantation in sheep [12,32]. In cow study, the results were also not encouraging [24,33]. To date, it has not been possible to perform whole ovary transplantation after cryopreservation in humans because of technical difficulties in cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Zhang

Ruan

et al. 2012

J Assist Reprod Genet

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Purpose The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation Methods Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured. Results When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols. Conclusion Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.

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Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Cited by 18 publications

References 29 publications

Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant

Complete protection against cryodamage of cryopreserved whole bovine and human ovaries using DMSO as a cryoprotectant

Cryoconservation of Chicken Blastodermal Cells: Effects of Slow Freezing, Vitrification, Cryoprotectant Type and Thawing Method During <I>In Vitro</I> Processing

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

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