Cryopreservation of the Mammalian Kidney

Fahy, Gregory M.; Ali, S.E.

doi:10.1006/cryo.1997.2026

Cited by 43 publications

(15 citation statements)

References 18 publications

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“…Cryprotectants are generally toxic to cells, and at the concentrations typically used for vitrification, almost anything except water is toxic to cells. Even the simple osmotic stresses associated with addition and removal of such massive amounts of additives can be lethal 15,17,19. The challenge of finding vitrification solutions that do not kill the cells they are intended to preserve, and the challenge of introducing those solutions into every part of a complex tissue without causing severe damage, remain formidable.…”

Section: Vitrificationmentioning

confidence: 99%

Cryo‐Injury and Biopreservation

Fowler

Toner

2006

Annals of the New York Academy of Sciences

214

177

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Mammalian cells appear to be naturally tolerant to cold temperatures, but the formation of ice when cells are cooled leads to a variety of damaging effects. The study of cryo-injury, therefore, becomes the study of when and how ice is formed both inside and outside the cell during cooling. Protectant chemicals are used to control or prevent ice formation in many preservation protocols, but these chemical themselves tend to be damaging. Cooling and warming rates also strongly affect the amount and location of ice that is formed. Through careful modification of these parameters successful cold preservation techniques for many cell types have been developed, but there are many more cell types that have defied preservation techniques, and the extension of cell-based techniques to tissues and whole organs has been very limited. There are many aspects to the damaging effects of ice in cells that are still poorly understood. In this brief article we review our current understanding of cellular injury and highlight the aspects of cellular injury during cryopreservation that are still poorly understood.

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Section: Vitrificationmentioning

confidence: 99%

Cryo‐Injury and Biopreservation

Fowler

Toner

2006

Annals of the New York Academy of Sciences

214

177

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“…The mere addition and removal of the chemicals can kill the cells due to osmotic stress, and the chemicals are toxic. 215,217,218 It seems clear that many cell types will never be successfully vitrified unless new, nontoxic cryoprotectants can be found, and it is not clear that this will even happen. Vitrification may ultimately be effective for the storage of certain organs whose cells can tolerate the required chemicals, but it is unlikely to be the best method for preserving the many different cell types the field of tissue engineering will require.…”

Section: Cryopreservation and Tissue Engineeringmentioning

confidence: 99%

Engineering Complex Tissues

2012

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“…Hypothermic storage is the currently preferred method to minimize such irreversible tissue damage [4, 5]. The quality and properties of the preservation solution play a key additional role.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of B2 Preservation Solution in Comparison to a Standard Solution (Histidine-Tryptophan-Ketoglutarate/Bretschneider) in a Model of Isolated Autologous Hemoperfused Porcine Kidney

Fehrenberg

Baeyer²,

Unger³

et al. 2004

Nephron Physiol

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Reperfusion injuries after organ transplantation affect graft function and influence long-term graft survival. As hypothermic storage, which minimizes the extent of unspecific tissue injury after ischemia and reperfusion, is significantly influenced by the composition of preservation solutions, strategies to optimize the different components may lead to longer graft survival. In the present study the effects of the preservation solution B2 on early renal function and histopathological changes were compared to histidine-tryptophan-ketoglutarate solution (HTK, Bretschneider) in a model of isolated blood-perfused porcine kidneys. B2-preserved kidneys displayed a lower renal resistance and significantly better creatinine clearance as compared to HTK. Mean differences were also found for filtration fraction and sodium fraction reabsorption. The functional data were also related to histopathological changes. Together, these data indicate that the recently developed preservation solution B2 offers new principles of preservation and is a useful preservation solution for experimental isolated perfused kidney models. B2 may also be an interesting model for optimizing preservation within other organ perfusion models.

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Cryopreservation of the Mammalian Kidney

Cited by 43 publications

References 18 publications

Cryo‐Injury and Biopreservation

Cryo‐Injury and Biopreservation

Engineering Complex Tissues

Protective Effects of B2 Preservation Solution in Comparison to a Standard Solution (Histidine-Tryptophan-Ketoglutarate/Bretschneider) in a Model of Isolated Autologous Hemoperfused Porcine Kidney

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