Cryopreservation of the human gut microbiota: Current state and perspectives

Smirnova, Daria V.; Zalomova, L. V.; Zagainova, A. V.; Макаров, В. Н.; Mezhevikina, L. M.; Фесенко, Е. Е.; Yudin, S. M.

doi:10.1016/j.ijmm.2019.06.001

Cited by 15 publications

(16 citation statements)

References 90 publications

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“…So far, different approaches to enhance the FMT concept were tested for the treatment of C. difficile infections (Chiu et al, 2021). The application of a processed, frozen and thawed donor stool resulted in recovery rates for CDI of up to 95% (Hamilton et al, 2012;Smirnova et al, 2019;Nicco et al, 2020). Further to that, different researchers investigated the treatment success with lyophilized faeces.…”

Section: Introductionmentioning

confidence: 99%

Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability

Haindl

Totzauer

Kulozik

2022

Microbial Biotechnology

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Faecal microbiota transplantation is an emerging medical concept for the treatment of gastrointestinal diseases. This concept, however, has disadvantages as low storability of stool and intensive donor screening. A solution to overcome these problems would be the preservation of an in vitro microbiota through freeze-drying. However, the influence of the entire preservation process, including cultivation and lyophilization, has not been assessed so far. In this study, the influences of the process steps cultivation, drying and re-cultivation were determined with cell count, production of metabolites, microbial composition and diversity in the system as evaluation criteria. All pH conditions resulted in stable, culturable communities after re-cultivation. Cell count, richness, diversity and microbial composition were affected by freeze-drying, but these effects were reversible and vanished during re-cultivation. Hence, the re-cultivated system did not differ from the system before drying. The metabolism, measured by short-chain fatty acids as indicators, showed slight changes due to natural dynamics. Consequently, the cultivation prior to drying was identified to have more influence than the drying itself on the preservation process and therefore the biggest potential for optimization. Hence, the highest similarity with the initial stool sample was obtained with pH 6.0 -6.5 during cultivation.

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Section: Introductionmentioning

confidence: 99%

Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability

Haindl

Totzauer

Kulozik

2022

Microbial Biotechnology

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“…Mainly low freezing temperature, ice crystal formation as well as glass transitions are causing cellular damage due to occurring cell stresses including cold, osmotic, mechanical as well as oxidant stress. During freezing, damages of membrane lipids, cell proteins and DNA is observed, leading to cell functionality losses and finally loss of viability (Fonseca et al 2006 , 2015b ; Smirnova et al 2019 ). The bacterial viability as well as their biological activity after freezing and frozen storage depends on: the strain, culture conditions, cryoprotectants and their formulations, freezing conditions and storage time (Fonseca et al 2001 , 2006 ; Smith and Ryan 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation and probiotic potential of lactic acid bacteria from swine feces for feed additive composition

Marchwińska

Gwiazdowska

2021

Arch Microbiol

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Animal microbiota is becoming an object of interest as a source of beneficial bacteria for commercial use. Moreover, the escalating problem of bacterial resistance to antibiotics is threatening animals and humans; therefore, in the last decade intensive search for alternative antimicrobials has been observed. In this study, lactic acid bacteria (LAB) were isolated from suckling and weaned pigs feces (376) and characterized to determine their functional properties and usability as pigs additives. Selection of the most promising LAB was made after each stage of research. Isolates were tested for their antimicrobial activity (376) and susceptibility to antibiotics (71). Selected LAB isolates (41) were tested for the production of organic acids, enzymatic activity, cell surface hydrophobicity and survival in gastrointestinal tract. Isolates selected for feed additive (5) were identified by MALDI-TOF mass spectrometry and partial sequence analysis of 16S rRNA gene, represented by Lentilactobacillus, Lacticaseibacillus (both previously classified as Lactobacillus) and Pediococcus genus. Feed additive prototype demonstrated high viability after lyophilization and during storage at 4 °C and − 20 °C for 30 days. Finally, feed additive was tested for survival in simulated alimentary tract of pigs, showing viability at the sufficient level to colonize the host. Studies are focused on obtaining beneficial strains of LAB with probiotic properties for pigs feed additive.

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“…One of the main reasons for the development of preservation methods of intestinal microorganisms is the possible production of therapeutic microbiota [66], such as fecal transplants and probiotics [67]. The microbiome is a unique natural resource that has possible medical applications.…”

Section: Preservation Of Intestinal Microorganismsmentioning

confidence: 99%

“…The microbiome is a unique natural resource that has possible medical applications. The result of the currently occurring human way of life entails the extinction of microbial species due to exposure to adverse environmental conditions such as diet, antibiotic use or stress [67][68][69][70]. Therefore, it is important to find suitable methods for preserving the intestinal microbiome to allow the timely restoration of the intestinal microflora with minimal safety risks.…”

Section: Preservation Of Intestinal Microorganismsmentioning

confidence: 99%

Distribution of Sulfate-Reducing Bacteria in the Environment: Cryopreservation Techniques and Their Potential Storage Application

et al. 2021

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Sulfate-reducing bacteria (SRB) are a heterogeneous group of anaerobic microorganisms that play an important role in producing hydrogen sulfide not only in the natural environment, but also in the gastrointestinal tract and oral cavity of animals and humans. The present review was written with the inclusion of 110 references including the time period from 1951 to 2021. The following databases were evaluated: Web of Science, Scopus and Google Scholar. The articles chosen to be included in the review were written mainly in the English and Czech languages. The molecular mechanisms of microbial cryoprotection differ depending on the environment where microorganisms were initially isolated. It was observed that the viability of microorganisms after cryopreservation is dependent on a number of factors, primarily colony age, amount of inoculum, cell size or rate of cooling, and their molecular inventory. Therefore, this paper is devoted to assessing the performance and suitability of various cryopreservation methods of intestinal bacteria, including molecular mechanisms of their protection. In order to successfully complete the cryopreservation process, selecting the correct laboratory equipment and cryopreservation methodology is important. Our analysis revealed that SRB should be stored in glass vials to help mitigate the corrosive nature of hydrogen sulfide, which can affect their physiology on a molecular level. Furthermore, it is recommended that their storage be performed in distilled water or in a suspension with a low salt concentration. From a molecular biological and bioengineering perspective, this contribution emphasizes the need to consider the potential impact associated with SRB in the medical, construction, and environmental sectors.

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Cryopreservation of the human gut microbiota: Current state and perspectives

Cited by 15 publications

References 90 publications

Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability

Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability

Isolation and probiotic potential of lactic acid bacteria from swine feces for feed additive composition

Distribution of Sulfate-Reducing Bacteria in the Environment: Cryopreservation Techniques and Their Potential Storage Application

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