Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience

Toker, Mehmed Berk; Alçay, Selim; Gökçe, Elif; Üstüner, Burcu

doi:10.1016/j.cryobiol.2016.05.001

Cited by 44 publications

(43 citation statements)

References 31 publications

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“…These undesirable effects may cause irreversible decrease on sperm function parameters (Nur et al, 2010; Ustuner et al, 2016). The success of sperm cryopreservation depends on the composition of the extenders which is a crucial factor to protect spermatozoon (Alcay, Gokce, et al, 2016; Toker, Alcay, Gokce, & Ustuner, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Lecithin has an important effect on the regulation of animal cells’ bio‐membrane (Gokce, Alcay, & Gul, 2017; Ustuner, Alcay, Nur, Sagirkaya, & Soylu, 2014). Therefore, it is used for sperm cryopreservation either as an extracted substance or as an ingredient of egg yolk (Toker et al, 2016; Ustuner et al, 2014). Lecithin protects the sperm plasma membrane by restoring the phospholipids lost during the heat shock (Toker et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post‐thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin‐based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 × 106 spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA‐FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post‐thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin‐based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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“…Thus, various extenders and cryoprotective agents have been investigated for the cryopreservation of ram semen (Nur et al, 2010;Najafi et al, 2013;Taşdemir et al, 2013). Previous studies have demonstrated the cryoprotectant properties of soybean-derived lecithin for protection of ram semen during freeze-thawing processes (MataCampuzano et al, 2015;Mehdipour et al, 2016;Najafi et al, 2014b;Toker et al, 2016). To the best of our knowledge, however, no studies have dealt with the supplementation of soybean lecithin-based extenders with different cryoprotectants (glycerol, ethylene glycol and DMSO).…”

Section: Discussionmentioning

confidence: 95%

“…However, the use of egg yolk has been associated with sanitary risks, including the production of harmful metabolites, toxins and the risk of infection and increases the agglutination and pre-capacitation of sperm, all resulting in reduced semen quality and damaging the fertilizing capacity of semen (Najafi et al, 2014b;Mehdipour et al, 2016). Egg yolk can be replaced by products of vegetable origin, such as soybean lecithin, which has been described as a suitable substitute for egg-yolk because it improves both the quality and fertilization ability of cryopreserved semen (Mata-Campuzano et al, 2015;Toker et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Najafi

Kia

Dodaran

et al. 2017

Animal Reproduction Science

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“…Low-molecular weight amino acids were widely used as additives in ram semen extenders. Among them methionine, cysteamine and cysteine were extensively studied and were indicated as supplements that can reduce malondialdehyde's levels and improve post-thaw ram semen parameters Najafi et al, 2014;Sharafi et al, 2015;Toker et al, 2016). However, there are relative to the aforementioned amino acids studies, which report negative effects of cysteamine on semen motility, mitochondrial activity and DNA integrity (Cirit et al, 2013; Mata-Campuzano et al,…”

Section: Ii) Non-enzymatic Antioxidantsmentioning

confidence: 99%

Current status and advances in ram semen cryopreservation

Ntemka

Tsakmakidis

Kiossis

et al. 2018

J Hellenic Vet Med Soc

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Ram semen cryopreservation contributes to genetic improvement through artificial insemination, eliminates geographical barriers in artificial insemination application and supports the preservation of endangered breeds thus the conservation of biodiversity. Sperm freezing process induces ultrastructural, biochemical and functional changes of spermatozoa. Especially, spermatozoa’s membranes and chromatin can be damaged, sperm membranes’ permeability is increased, hyper oxidation and formation of reactive oxygen species takes place, affecting fertilizing ability and subsequent early embryonic development. Aiming to improve ram frozen-thawed semen’s fertilizing capacity, many scientific investigations took place. Among them the composition of semen extenders, was a main point of interest. Semen preservation extenders regulate and support an environment of adequate pH and buffering capacity to protect spermatozoa from osmotic and cryogenic stress. Therefore, permeating (glycerol, dimethyl sulfoxide) and non-permeat ing (egg yolk, skimmed milk) cryoprotectants, sugars (glucose, lactose, trehalose, raffinose), salts (sodium citrate, citric acid) and antioxidants (amino acids, vitamins, enzymes) have been added and tested. Moreover, semen dilution rate, storage temperature, cooling rate and thawing protocol, are also some key factors that have been studied. The research results of this scientific topic are encouraging, not only about the freezing and thawing procedures, but also about the improvement of the additives’ properties. However, further research is needed to enhance the fertilizing ability of ram frozen-thawed semen, making its use practical in sheep reproductive management by the application of cervical artificial insemination.

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Cryopreservation of ram semen with antioxidant supplemented soybean lecithin-based extenders and impacts on incubation resilience

Cited by 44 publications

References 31 publications

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Current status and advances in ram semen cryopreservation

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