Cryopreservation of rainbow trout semen using a glucose-methanol extender

Motility and fertilizing ability of cryopreserved Caspian brown trout (Salmo trutta caspius) sperm: Effect of post-thaw storage time and different sperm-to-egg ratios

“…This method secures good fertilization rates, but a high sperm-to-egg ratio (6.2 Â 10 6 ) is necessary. On the other hand, recent studies shown that storage times up to 15 min did not affect the post-thaw fertility of salmonid semen and even times of 30, 45 and 60 min have been used [2,10]. Thus, the use of lower than previously published sperm-to-egg ratios and also higher post-thaw storage time, leading to high fertilizing ability of Caspian brown trout semen is necessary.…”

“…Furthermore, an elaborated procedure for securing low sperm-to-egg ratio would improve the effectiveness of fish cryopreservation and could lead to the implementation of this procedure in hatchery conditions [2]. Previously, an effective cryopreservation procedure was reported for Caspian brown trout sperm [12].…”

“…Therefore, this study was carried out to test the influence of post-thaw storage time on motility parameters [percentage of motility (MOT), straight line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), linearity (LIN) amplitude of lateral head displacement (ALH)] and fertilization ability (at the eyed and hatching stages) of Caspian brown trout semen. Furthermore, we examined the effect of sperm-to-egg ratios of 100,000:1, 300,000:1 and 600,000:1 [2] on fertilizing ability of cryopreserved Caspian brown trout semen.…”

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

“…The latter methods employed extenders consisting of potassium ions and sucrose buffered by Tris-HCl in methanol. Simple extenders containing glucose and methanol have been recently used for cryopreservation of salmonid fish sperm [10,13,22]. These results indicate that the buffering of extenders for cryopreservation to specific pH and the addition of potassium ions may not be necessary.…”

“…The experiment was conducted on10.03.2014. The effect of glucose concentration (0, 0.10, 0.15, 0.20 and 0.30 M) in 15% methanol (semen: extender 1:1) on the sperm motility of fresh-diluted and cryopreserved semen was tested on three individual samples of sperm (n = 3).…”