Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

Kutluyer, Filiz; Kayim, Murathan; Öğretmen, Fatih; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros

doi:10.1016/j.cryobiol.2014.10.005

Cited by 67 publications

(44 citation statements)

References 27 publications

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“…In accord with previous studies Kutluyer, Kayim, Öğretmen, Büyükleblebici, & Tuncer, 2014), antioxidants did not appear to influence the fertilizing ability of sturgeon sperm following the freeze-thaw process. Further investigation is needed to determine whether different antioxidant doses might show greater effects.…”

Section: Discussionsupporting

confidence: 91%

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

et al. 2017

Reprod Domestic Animals

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High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25 U/ml, 0.25-0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermatozoan motility in A. sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.

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Section: Discussionsupporting

confidence: 91%

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

et al. 2017

Reprod Domestic Animals

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“…Oxidative damage causes lower motility, DNA damages, and lipid peroxidation [55]. Membrane phospholipids are related to cell freezability, which affects sperm motility and mitochondrial potential [16, 56]. SOD2 is a major antioxidant, and it reacts with ROS directly to protect against oxidative stress [40].…”

Section: Discussionmentioning

confidence: 99%

Proteomic identification of cryostress in epididymal spermatozoa

Yoon

Rahman

Kwon

et al. 2016

J Animal Sci Biotechnol

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Background: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis (2-DE) results using two commercial antibodies. Results: Cryopreservation reduced viability (%), motility (%), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and capacitated spermatozoa, whereas straightness (STR) and the acrosome reaction increased after cryopreservation (P < 0.05). Nine proteins were differentially expressed (two proteins decreased and seven increased) (>3 fold, P < 0.05) before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation, and the guanylate cyclase pathway.

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“…The plasma membranes of spermatozoa contain large amounts of polyunsaturated fatty acid (PUFA), which are very susceptible to oxidative damage (Martínez‐Páramo, Diogo, Dinis, Herráez, Sarasquete & Cabrita ; Kutluyer et al . ). Previously, it was demonstrated that the cryopreservation may cause damages to the spermatozoa, probably due to the production of reactive oxygen species, such as superoxide and hydrogen peroxide (Martínez‐Páramo et al .…”

Section: Discussionmentioning

confidence: 97%

“…Cabrita, Anel & Herráez ; Horvath, Wayman, Urbányi, Ware, Dean & Tiersch ; Vuthiphandchai et al . ; Boryshpolets, Dzyuba, Rodina, Alavi, Gela & Linhart ; Alavi, Hatef, Psenicka, Kaspar, Boryshpolets, Dzyuba, Cosson, Bondarenko, Rodina, Gela & Linhart ; Kutluyer, Kayım, Öğretmen, Büyükleblebici & Tuncer ; Judycka, Szczepkowski, Ciereszko & Dietrich ). The efficiency of CPAs used in sturgeon sperm cryopreservation has been found to be species‐specific (Alavi et al .…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Persian sturgeon (Acipenser persicus) sperm: effects of cryoprotectants, antioxidant, membrane stabilizer, equilibration time and dilution ratio on sperm motility and fertility

2015

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Three experiments were performed to develop protocols for cryopreservation of Persian sturgeon Acipenser persicus, sperm. In the first experiment, sperm from six males was individually split in three subsamples and cryopreserved using Modified Tsvetkova's extender (mT) supplemented with dimethyl sulfoxide (DMSO), methanol (MeOH), glycerol (Gly) and ethylene glycol (EG) at concentration of 5%, 10%, 15% and 20%. In the second set of experiments, the effects of six equilibration times (0, 5, 10, 20, 40 and 60 min) and dilution ratios (volume sperm: volume extender 1:0.5, 1:1, 1:2, 1:3, 1:5 and 1:10) and the additive advantage of bovine serum albumin (BSA; 0, 2.5, 5 and 10 mg mL À1 ) and ascorbic acid (0, 2.5, 5 and 10 U mL À1 ), on the post-thaw survival of sperm (triplicate set of six fish) were evaluated. Then, sperm was diluted in 1:1 mT extender with 10 mg mL À1 BSA with selected cryoprotectants (15% MeOH and 10% DMSO) for 5 min. After a month of storage in liquid nitrogen, post-thawed sperm motility; fertilization and hatching rate and viability of derived larvae were measured (Exp.3). Evaluation of cryoprotectants efficiency showed that MeOH 15% and DMSO 10% were suitable for cryopreservation of Persian sturgeon sperm. Gly and EG resulted in very low post-thaw motility rates even at lowest concentration. No significant difference was observed among the four different equilibration times (0, 5, 10, 20 min) (P > 0.05) although higher equilibration times than 20 min resulted low post-thaw motility (P < 0.05). The motility of frozen-thawed sperm did not significantly change when dilution ratio was increased from 1:0.5 to 1:3 (P > 0.05). However, higher dilution ratios (1:5 and 1:10) reduced the percentage of motile sperm. Supplementation of the cryoprotectant solution with 10 mg mL À1 BSA significantly improved post-thaw motility (P < 0.05), but ascorbic acid did not improve post-thaw motility (P > 0.05). The results of experiment 3 showed that the highest fertilization (30.2 AE 5.75) and hatching rates (28.2 AE 5.25) were observed when samples were frozen with 15% MeOH (P > 0.05). Our study indicates that the use of mT extender consisting of 10 mg mL À1 BSA in 15% MeOH diluted with sperm at 1:1 ratio for 5 min can be recommended cryopreservation method for Persian sturgeon sperm.

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Cryopreservation of rainbow trout Oncorhynchus mykiss spermatozoa: Effects of extender supplemented with different antioxidants on sperm motility, velocity and fertility

Cited by 67 publications

References 27 publications

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Proteomic identification of cryostress in epididymal spermatozoa

Cryopreservation of Persian sturgeon (Acipenser persicus) sperm: effects of cryoprotectants, antioxidant, membrane stabilizer, equilibration time and dilution ratio on sperm motility and fertility

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