Cryopreservation of primed tobacco seeds

Lopes, Camila Aparecida; Carvalho, María Laene Moreira de; Souza, Ana Cristina de; Oliveira, João Almir; Andrade, Dayliane Bernardes de

doi:10.1590/2317-1545v40n4207216

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…Seeds were harvested on plants before pod shattering and removed from fruits, after that, were stored in cold chamber for about 20 days, when the experiments were started. Priming consisted in five solutions: distilled water, 0.2% potassium nitrate (Cardoso et al, 2015), 0.5 mmol.L -1 spermidine (SPE) (Hussain et al, 2015;Lopes et al, 2018), 50 mg.L -1 gibberellin (GA) (Batista et al, 2015;Cardoso et al, 2015), and 0.10 mmol.L -1 sodium nitroprusside (SNP) (Faraji and Sepehri, 2018). Treatments consisted in immersion of 40 g seeds in 400 mL of above-described solutions in Erlenmeyer, being the solution kept aerated with the support of an air compressor.…”

Section: Methodsmentioning

confidence: 99%

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

et al. 2022

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As forage production increase, high-quality seeds demand follows. Priming have been reported as a technique for improve seed quality and stress tolerance. The aim of this study was to evaluate the priming agents effect on Urochloa ruziziensis seed germination and seedling establishment under water deficit and salt stress. A completely randomized experiment assessing six priming agents and three germination conditions were established. We evaluated: seed water content; germination percentage and speed index; viability of seeds remaining from the germination test; seedling length; antioxidant enzymatic activity; and malondialdehyde quantity. Seeds primed under water deficit and salt stress showed better physiological performance than dry seeds. There was a significant increase in seed germination percentage and speed and in seedling roots and shoots length. Sodium Nitroprusside shows significant potential for use in the physiological priming of U. ruziziensis seeds.

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Section: Methodsmentioning

confidence: 99%

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

et al. 2022

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“…Each treatment was primed in a single Erlenmeyer flask, without replications. The priming solutions used were distilled water (water), 0.2% potassium nitrate solution (KNO 3 ) (Cardoso et al, 2014;Cardoso et al, 2015), 0.5 mmol.L −1 spermidine solution (SPER) (Hussain et al, 2015;Lopes et al, 2018), 50 mg.L −1 pure gibberellin solution (GB) (Batista et al, 2015;Cardoso et al, 2015), and 0.10 mmol.L −1 sodium nitroprusside solution (SNP) (Ataíde et al, 2015;Silva et al, 2015;Faraji et al, 2018). The seeds used as a control were not primed.…”

Section: Methodsmentioning

confidence: 99%

“…Various authors have found that the positive effects of the SPER are associated with maintenance of membrane integrity, regulation of gene expression, breaking of dormancy, and seed germination, flower and fruit development, and senescence (Gupta et al, 2014;Lopes et al, 2018;Yalamalle et al, 2019). In addition, the SPER also plays a crucial role in tolerance to abiotic stresses (Yalamalle et al, 2019).…”

Section: Journal Of Seedmentioning

confidence: 99%

Protective action of priming agents on Urochloa brizantha seeds under water restriction and salinity conditions

et al. 2021

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Analyses of germination of seeds under artificial stress conditions are tools for a better understanding of the survival and adaptation capacity of species under natural stress conditions. Therefore, the aim of this study was to evaluate the effect of priming on seed germination, on breaking of dormancy, and on the length of Urochloa brizantha seedlings under water restriction and salt stress conditions. A completely randomized experimental design was used with four replications in a 3 × 6 factorial arrangement consisting of 3 germination conditions (water, water restriction with PEG, and salinity with NaCl) and 6 treatments [five priming solutions (distilled water, potassium nitrate, spermidine, gibberellin, and sodium nitroprusside) and a control (unprimed seeds)]. The following determinations were made: seed moisture content after priming (before drying) and after drying the seeds, germination test at seven and twenty-one days after sowing, germination speed index, viability by the tetrazolium test of the seeds remaining from the germination test, and seedling length. Priming is effective in minimizing the negative effects of water restriction and salinity. Use of sodium nitroprusside as a priming solution results in a lower percentage of dormant seeds and greater germination, vigor, and plant development.

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“…Priming with ascorbic acid and sodium nitroprusside allowed an improved performance of stored sunflower seeds, possibly due to a boost in the antioxidant defense (Pereira et al 2022 ). Priming with water and spermidine, followed by drying in presence of silica gel and a saturated saline solution was applied to tobacco seeds that were cryopreserved in liquid nitrogen for 24 h without loss of germinability (Lopes et al 2018 ). These findings suggest that the longevity of primed seeds can be improved by selecting optimal priming parameters, storage, and post-storage conditions (Fabrissin et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular dynamics of seed priming at the crossroads between basic and applied research

Pagano¹,

Macovei²,

Balestrazzi³

2023

Plant Cell Rep

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Key message The potential of seed priming is still not fully exploited. Our limited knowledge of the molecular dynamics of seed pre-germinative metabolism is the main hindrance to more effective new-generation techniques. Abstract Climate change and other recent global crises are disrupting food security. To cope with the current demand for increased food, feed, and biofuel production, while preserving sustainability, continuous technological innovation should be provided to the agri-food sector. Seed priming, a pre-sowing technique used to increase seed vigor, has become a valuable tool due to its potential to enhance germination and stress resilience under changing environments. Successful priming protocols result from the ability to properly act on the seed pre-germinative metabolism and stimulate events that are crucial for seed quality. However, the technique still requires constant optimization, and researchers are committed to addressing some key open questions to overcome such drawbacks. In this review, an update of the current scientific and technical knowledge related to seed priming is provided. The rehydration–dehydration cycle associated with priming treatments can be described in terms of metabolic pathways that are triggered, modulated, or turned off, depending on the seed physiological stage. Understanding the ways seed priming affects, either positively or negatively, such metabolic pathways and impacts gene expression and protein/metabolite accumulation/depletion represents an essential step toward the identification of novel seed quality hallmarks. The need to expand the basic knowledge on the molecular mechanisms ruling the seed response to priming is underlined along with the strong potential of applied research on primed seeds as a source of seed quality hallmarks. This route will hasten the implementation of seed priming techniques needed to support sustainable agriculture systems.

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Cryopreservation of primed tobacco seeds

Cited by 4 publications

References 14 publications

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

Priming Urochloa ruziziensis (R.Germ. & Evrard) seeds with signalling molecules improves germination

Protective action of priming agents on Urochloa brizantha seeds under water restriction and salinity conditions

Molecular dynamics of seed priming at the crossroads between basic and applied research

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