Cryopreservation of Phytodiversity: A Critical Appraisal of Theory &amp; Practice

Benson, Erica E.

doi:10.1080/07352680802202034

Cited by 237 publications

(179 citation statements)

References 258 publications

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“…Besides long-term storage, cryopreservation can also be used for virus elimination, a method named cryotherapy (Benson 2008b;Valkonen 2009a, 2009b;Wang et al 2008aWang et al , 2008bWang et al , 2009). Cooling and freezing of explants down to LN temperatures facilitates viral elimination in larger meristems, because many viruses are unable to infect the meristem proper, and virus-free plants can be grown from excised apices of small size (Martin and Postman 1999).…”

Section: Cryotherapymentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in

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Section: Cryotherapymentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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“…One of the advantages of tissue culture is the capacity to preserve germplasm in vitro for long periods without the large investments in land, labour, water, fertiliser, and pesticide that would be required for plantation-or nursery-based germplasm archives [42,[51][52][53]178,179]. In vitro storage can also delay the maturation of valuable clones, especially if their shoots or callus are stored under minimal-growth or nil-growth conditions [39,40,42,[180][181][182].…”

Section: In Vitro Preservationmentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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“…Sun (2000); Walters (2007); Buitink & Leprince (2008) ascertained that glass stability is not upheld per se, it is based on groups of different biomolecules, linked by hydrogen bonds with water molecules. Bryant et al (2001) and Benson (2008) suggested that the formation of a glassy matrix (i.e., vitrification) in could represent a strategy for desiccation tolerance and storage stability, in general. Vitrification achieves a high viscosity without a great deal of molecular reorganization (Hatanaka & Sugawara, 2010) and, therefore, limits major changes in the cellular structures (Buitink & Leprince, 2008;Walters et al, 2010).…”

Section: The Thermodynamics Of Seed and The Maintenance Of Seed Viabimentioning

confidence: 99%