Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation–vitrification and encapsulation–dehydration

Wang, Qiaochun; Laamanen, Jaana; Uosukainen, Marjatta; Valkonen, Jari P. T.

doi:10.1007/s00299-005-0936-x

Cited by 83 publications

(58 citation statements)

References 19 publications

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“…Furthermore, DAB has recently applied cryotherapy as a novel means for eradication of viruses and phytoplasma from vegetatively propagated plants, which results in virusfree plants with a much higher frequency than the previously used methods . At the same time, Laukaa Research and Elite Plant Unit of MTT has developed cryopreservation techniques for perennial horticultural plants (Wang et al 2005) and in collaboration with DAB introduced the cryotherapy technique to healthy plant production. Consequently, Finland has become a forerunner in the use of this approach.…”

Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

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Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

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“…These authors found that only the shoot apices with water content lower than 22.5% survived. Wang, Laamanen, Uosukainen, & Valkonen (2005) observed that sodium alginate capsules containing apical buds of Rubus idaeus L., pre-cultured in MS medium containing sucrose, had an initial moisture content of 66%, and the water content decreased to 22.2% in the first 4 hours in an airflow hood, and to 14.3% after 8 hours of dehydration. According to these authors, the highest recovery rates of the apical buds were obtained by dehydration for at least 3 hours in an airflow hood.…”

Section: Encapsulation-dehydrationmentioning

confidence: 99%

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

Prudente

Paiva

Nery

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study's objective was to assess the behavior of lateral bud's regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants.Keywords: Mangabeira, recalcitrant species, long-term conservation, dehydration.Comportamento de gemas laterais de Hancornia speciosa após criopreservação por encapsulamento-vitrificação RESUMO. Hancornia speciosa é uma frutífera do Cerrado com elevado potencial econômico. Entretanto, o extrativismo desordenado causou a redução populacional em sua área endêmica. Além disso, a recalcitrância da semente afeta negativamente sua conservação convencional. Buscando alternativas de conservação para essa espécie, objetivou-se avaliar a regeneração das gemas laterais após a técnica de encapsulamento-vitrificação. Cápsulas de alginato de sódio contendo gemas laterais foram pré-cultivadas em meio WPM acrescido de 1,0 M de glicerol e, posteriormente, imersas em diferentes concentrações de sacarose (0,3; 0,75 e 1,0 M) por 24 ou 48 horas. As cápsulas foram submetidas à desidratação em sílica gel ou em fluxo laminar por 0, 1, 2 e 3 horas antes de sua incubação em diferentes tempos de PVS2 (0, 15, 30, 60 e 120 minutos). Elevada porcentagem de regeneração de gemas laterais foi observada após a criopreservação de cápsulas tratadas com 0,75 M de sacarose + 1,0 M de glicerol por 24 horas, associado com a desidratação em fluxo laminar (1 hora) e imersão em PVS2 (15 minutos). O encapsulamento-vitrificação é eficiente para a conservação de longo prazo e permite a obtençao de altas taxas de sobrevivência após a criopreservação de explantes sensíveis (gemas laterais) de Hancornia speciosa.Palavras-chave: Mangabeira, espécie recalcitrante, conservação em longo prazo, desidratação.

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“…A este respecto, se han probado técnicas como la organogénesis directa e indirecta utilizando hojas de diferentes variedades comerciales (Isac, 2009) y la criopreservación de brotes apicales (Wang et al, 2005;Gupta y Reed, 2006). Algunos sistemas para la micropropagación de frambuesa han utilizadoel medio de cultivo MS (Muashige y Skoog, 1692) semisólido y líquido en inmersión temporal complementado con bencil amino purina (BAP), ácido giberélico (AG 3 ) y ácido ascórbico (Jones y Flores, 2007); también se reporta el uso de N 6 -benciladenina, tidiazuron y zeatina (Reed, 1991;Popescue Isac, 1999;González et al, 2000;Vujović et al, 2009).…”

Section: Introductionunclassified

“…In this regard, techniques like direct and indirect organogenesis have been used, using leaves from different commercial varieties (Isac, 2009) and cryopreservation of apical shoots (Wang et al, 2005;Gupta and Reed, 2006). Some systems for raspberry micro propagation have used MS medium (Muashige and Skoog, 1692) semisolid and liquid in temporary immersion supplemented with benzyl amino purine (BAP), gibberellic acid (GA 3 ) and ascorbic acid (Jones and Flores, 2007); the use of N 6 -benzyladenine, thidiazuron and zeatin (Reed, 1991;Popescue Isac, 1999;González et al, 2000;Vujović et al, 2009) has also been reported.…”

Section: Introductionmentioning

confidence: 99%

La elongación de brotes adventicios de frambuesa (Rubus ideaus L.) es influenciada por brasinosteroides

Robres-Torres

López-Medina

Rocha-Granados

2017

Remexca

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ResumenLa técnica de cultivo in vitro fue utilizada para estudiar el efecto de la combinación de benciladenina (BA) y brasinolido (BL), dos reguladores de crecimiento vegetal, en la formación y elongación de brotes adventicios de frambuesa (Rubus idaeus L.). También se evaluó el enraizamiento de los brotes regenerados tanto en condiciones in vitro como ex vitro. Tres selecciones de frambuesa, UMC-702, UMC-706 y UMC-708, generadas de un programa de mejoramiento genético en la Facultad de Agrobiología, fueron seleccionadaspara llevar a cabo esta investigación. En la fase de multiplicación, el medio MS fue suplementado con diferentes combinaciones de bencialadenina (0 y 1.5 mg L -1 ) y brasinolido (0, 0.00001 y 0.00002 µM). La formación de brotes adventicios no fue dependiente de la combinación de los reguladores; sin embargo, la elongación de los mismos fue significativamente influenciada por el genotipo y el uso de brasinolido (BL); los mejores resultados se obtuvieron con la selección UMC-706 expuesta a 0.00002 µM de BL. El enraizamiento de los brotes adventicios fue mejor en condiciones ex vitro que in vitro, con mayor formación y elongación tanto de raíz como de los brotes; ello permitió una mejor aclimatación y adaptación de las plantas, lo que significa un ahorro tanto en tiempo como en costos de labor para la micropropagación de frambuesa. Así, este es el primer reporte del uso de brasinosteroides en frambuesa. AbstractIn vitro culture techniques have been used to study the effect of the combination of benzyladenine (BA) and brassinolide (BL), two plant growth regulators, in the formation and adventitious shoot elongation of raspberry (Rubus idaeus L.). Rooting of regenerated shoots both in vitro and ex vitro conditions were evaluated. Three selections of raspberry, UMC-702 UMC-706 and UMC-708, generated from a breeding program at the Faculty of Agrobiology, were selected to conduct this research. In the multiplication phase, the MS medium was supplemented with different combinations of benzyladenine (0 and 1.5 mg L -1 ) and brassinolide (0, 0.00001 and 0.00002 µM). Adventitious shoot formation was not dependent on the combination of regulators; however, the elongation of it was significantly influenced by genotype and use of brassinolide (BL); the best results were obtained with UMC-706 exposed to 0.00002 µM of BL. Rooting of adventitious shoots were better in ex vitro than in vitro conditions, with higher formation and elongation both root and shoots; allowing a better acclimatization and adaptation of plants, which means savings in time and labor for micro propagation of raspberry. So, this is the first report on the use of brassinosteroids in raspberry.

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Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation–vitrification and encapsulation–dehydration

Cited by 83 publications

References 19 publications

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

<b>Behavior of lateral buds of <i>Hancornia speciosa</i> after cryopreservation by encapsulation-vitrification

La elongación de brotes adventicios de frambuesa (Rubus ideaus L.) es influenciada por brasinosteroides

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