2006
DOI: 10.1016/j.jhep.2005.07.008
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Cryopreservation of hepatic stellate cells

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Cited by 13 publications
(7 citation statements)
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“…The cryopreservation of human hepatocytes shows greatest success with a progressive freezing, with its slow freezing rate, following a 30-min preincubation at 37°C and could potentially be used in cell banking (2). Work has been done also involving the cryopreservation of hepatic stellate cells (26), which provide critical paracrine signaling for the hHBs. Here, both cooling rates and cryoprotectants were investigated and found to have a clear effect on the resulting viability and cell survival.…”
Section: Discussionmentioning
confidence: 99%
“…The cryopreservation of human hepatocytes shows greatest success with a progressive freezing, with its slow freezing rate, following a 30-min preincubation at 37°C and could potentially be used in cell banking (2). Work has been done also involving the cryopreservation of hepatic stellate cells (26), which provide critical paracrine signaling for the hHBs. Here, both cooling rates and cryoprotectants were investigated and found to have a clear effect on the resulting viability and cell survival.…”
Section: Discussionmentioning
confidence: 99%
“…A recent report describes a cryopreservation technique for freezing primary stellate cells using dimethyl sulfoxide (442). If reproducible in other laboratories, the technique will also accelerate progress by enabling the sharing of cells between investigators, especially those without the means to isolate their own primary cells.…”
Section: B Cell Linesmentioning
confidence: 99%
“…CRP2 antibodies were raised against the epitope GIKPESAQPHRPTTN, corresponding to amino acid 89-103 of murine CRP2, linked to KLH. 18 MMP-9 IgGs were purchased from R&D Systems (Wiesbaden, Germany), CD45 IgGs from BD (Heidelberg, Germany), F4/80 IgGs from Dianova (Hamburg, Germany), and myeloperoxidase IgGs from Abcam (Cambridge, UK). Alexa 468-and Alexa 488-conjugated secondary antibodies were purchased from Molecular Probes (Eugene, OR, USA).…”
Section: Immunofluorescencementioning
confidence: 99%