Cryopreservation of epididymal stallion sperm

Olaciregui, Maite; Gil, L.; Montón, A.; Luño, Victoria; Jerez, R. A.; Martí, J.I.

doi:10.1016/j.cryobiol.2013.12.009

Cited by 43 publications

(30 citation statements)

References 28 publications

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“…The data reported in the present study showed similar postthawing values compared with those of equine ejaculated semen with the same extender [34]. On the other hand, the data on sperm characteristics after cryopreservation in both ISs and HSs were higher than those reported in recent studies on equine epididymal spermatozoa [12,13]. In these studies, a similar base extender was used (INRA96), but a higher concentration of glycerol was used (5% vs. 2.5%) making a direct comparison of the results difficult.…”

Section: Discussioncontrasting

confidence: 46%

“…Recently, articles reported the collection of spermatozoa from the cauda epididymis in both horses and donkeys [9][10][11][12][13]. These studies were performed in experimental conditions, with normal and healthy males undergoing elective castration.…”

Section: Introductionmentioning

confidence: 97%

“…Furthermore, unlike other studies [12,13], differences between sperm characteristics in epididymal samples from ill or healthy stallions (ISs and HSs, respectively) were evaluated using objective systems, such as the computer-assisted sperm analyzer (CASA) system and flow cytometry, which allow the detection of fine differences between samples [14].…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

et al. 2016

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Section: Discussioncontrasting

confidence: 46%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

et al. 2016

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“…En un estudio con el hipopótamo pigmeo (Choeropsis liberiensis), a partir de muestras de semen obtenidas por electro-eyaculación, se encontraron valores promedio para MT, IA, MA y HOS, de 75.7% ± 6.9%; 87.8% ± 1.2%; 52.7% ± 4.3% y 79.3% ± 4.4%, respectivamente (Saragusty et al 2010a). La recuperación exitosa de espermatozoides directamente desde los epidídimos ha sido realizada en diferentes especies de mamíferos, en los que se destacan bovinos, equinos y caninos (Turri et al 2012;Olaciregui et al 2014, Mota et al 2014. Para el caso del hipopótamo común, un estudio reportó la extracción de espermatozoides desde la cola del epidídimo y su posterior evaluación, con resultados para MT, IA, MA y HOS de 88.1 ± 4.2%; 87.7 ± 1.8%; 51.6 ± 4.1% y 83.3 ± 1.8%, respectivamente (Saragusty et al 2010b).…”

Section: Discussionunclassified

Evaluación de la calidad espermática epididimal en hipopótamos Hippopotamus amphibius (Artiodactyla: Hippopotamidae) ubicados en el Magdalena Medio, Colombia

Betancur¹,

Giraldo²,

Suarez³

2016

AZM

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. Evaluación de la calidad espermática epididimal en hipopótamos Hippopotamus amphibius (Artiodactyla: Hippopotamidae) ubicados en el Magdalena Medio, Colombia. Acta Zoológica Mexicana (n. s.), 32(2): 158-167. RESUMEN. El hipopótamo común (Hippopotamus amphibius)es un mamífero del orden Artiodactyla, que habita en África, con una población decreciente a nivel global. Con fines de conservación genética, se han realizado procesos de biotecnología reproductiva, entre los que se reporta, la recuperación de espermatozoides por métodos como la electroeyaculación y la extracción desde epidídimos. Este mamífero es considerado una especie invasora exótica y fue introducido en Colombia en 1985; actualmente se reproduce de forma descontrolada en la región del Magdalena Medio. Como medida de control poblacional, se ha realizado la esterilización quirúrgica de algunos ejemplares. El objetivo de este estudio fue evaluar diferentes parámetros espermá-ticos e histológicos, relacionados con la calidad seminal epididimal de hipopótamos comunes ubicados en el Magdalena Medio, Colombia. Dos hipopótamos fueron orquiectomizados quirúrgicamente y los espermatozoides fueron recuperados por los métodos combinados de lavado retrógrado e inyección de medio. Se realizó la evaluación de la movilidad (sistema SCA ® ), la vitalidad espermática y la morfología espermática (prueba supravital), el potencial de membrana mitocondrial (JC-1), la integridad acrosómica (FITC-PNA), y la integridad estructural (SYBR14/IP) y funcional (prueba HOS) de la membrana plasmática. Asimismo, se realizó un análisis histológico de testículos y epidídimos. A excepción de la movilidad progresiva, la integridad funcional de membrana y la morfología espermática, se hallaron resultados muy similares para los parámetros de calidad seminal de ambos individuos. Las anormalidades espermáticas predominantes fueron gota citoplasmática y cola enrollada. El análisis histológico evidenció un proceso activo de espermatogénesis. Se concluye que la extracción epididimal de espermatozoides, posterior a la orquiectomía de hipopótamos comunes ubicados en un hábitat no nativo, permite obtener muestras espermáticas de buena calidad. Palabras clave: Colombia, cinética espermática, histología. Restrepo ABSTRACT. Common hippopotamus (Hippopotamus amphibius)is a mammal of the Artiodactyla order, living in Africa, with a declining population globally. With genetic conservation purposes, there have been processes of reproductive biotechnology, among which it is reported, recovery of sperm by methods as electroejaculation and extraction from the epididymis. This mammal is considered an exotic and invasive species was introduced in Colombia 1985; currently it reproduces out of control in the Middle Magdalena region. As a means of population control, it has performed the surgical sterilization of some specimens. The aim of this study was to evaluate different sperm and histological parameters related with epididymal semen quality of common hippopotamus located in the Colombian Middle Magdalen...

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“…It has been shown that viable sperm cells can be recovered from the cauda epididymis in bulls (Alapati et al, 2009; Ardon e Suarez, 2013), stallions (Weiss et al, 2008;Olaciregui et al, 2014), dogs (Hishinuma e Sekine, 2004) and rams (Kaabi et al, 2003). The use of genetic material obtained from the epididymis is particularly important following the sudden death of breeding livestock or in the preservation of endangered species.…”

Section: Introductionmentioning

confidence: 99%

Viability of ovine spermatozoa collected from epididymides stored at 18°-25°C for 48 hours post mortem

Bergstein-Galan

Weiss

Barbosa

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25°C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0±1.9%) compared to AV (86.4±1.0%). PM decreased (P<0.05) from 12 hours after death (31.3±4.0%) compared to AV group (73.2±1.4%). The percentage of viable cells in HOST decreased (P<0.05) in the G48 (60.0±8.9%). Spermatozoa recovery was lower (P<0.05) 48 hours after death (2064.2±230.7 x 10 6 spermatozoa) compared to G0(2623.6±288.4 x 10 6 spermatozoa). In conclusion, under the conditions of this study, it would be possible to use epididymal spermatozoa recovered up to 24 hours after death for artificial insemination or in vitro fertilization; however, fertility trials are necessary to prove this hypothesis. Keywords: ram, epididymis, room temperature RESUMO Os objetivos deste estudo foram avaliar o período pelo qual era possível recuperar espermatozoides ovinos viáveis da cauda de epidídimos mantidos em temperatura ambiente (18-25°C). O sêmen coletado em vagina artificial (AV) foi utilizado como controle. Os espermatozoides foram coletados dos epidídimos à zero hora (G0), às seis (G6), 12 (G12), 24 (G24) e 48 (G48) horas

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Cryopreservation of epididymal stallion sperm

Cited by 43 publications

References 28 publications

Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

Evaluación de la calidad espermática epididimal en hipopótamos Hippopotamus amphibius (Artiodactyla: Hippopotamidae) ubicados en el Magdalena Medio, Colombia

Viability of ovine spermatozoa collected from epididymides stored at 18°-25°C for 48 hours post mortem

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