Cryopreservation of epididymal sperm from ibexes (Capra pyrenaica) using short equilibration time with glycerol

Pradieé, J.; Esteso, M.C.; Castaño, C.; Toledano-Dı́az, A.; Santiago-Moreno, J.

doi:10.1016/j.theriogenology.2014.05.012

Cited by 16 publications

(5 citation statements)

References 22 publications

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“…Phylogenetic relationships between the mouflon and domestic sheep might explain the similarity in the response of their spermatozoa. Although shortening the equilibration time improves the results obtained with ibex epididymal spermatozoa (Pradiee et al., ), the present data suggest that mouflon spermatozoa collected by electroejaculation require a long equilibration period in the presence of cryoprotectant.…”

Section: Discussioncontrasting

confidence: 47%

Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification

et al. 2016

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This work examines the effectiveness of a TCG (Tris, citric acid, glucose, 6% egg yolk and 5% glycerol) and a TEST (TES, Tris, glucose, 6% egg yolk and 5% glycerol) sperm extender in the freezing of mouflon spermatozoa at slow cooling rates, using different pre-freezing equilibration times (2-3 hr). It also examines the tolerance of mouflon spermatozoa to different concentrations of cryoprotectants (5, 10, 20% glycerol; 5%, 10%, 20% dimethyl sulfoxide; 6% polyvinylpyrrolidone) and/or sucrose (100, 300, 500 mm). The highest quality (p < .01) thawed spermatozoa were obtained when using the TEST extender and an equilibration time of 3 hr. Sperm motility and membrane integrity were strongly reduced when using rapid freezing rates (60-85°C min ), independent of the concentration of cryoprotectants. The lowest sucrose concentration (100 mm) provided the highest (p < .05) percentage of motile spermatozoa and live spermatozoa with an intact acrosome. Vitrified-warmed sperm variables were at their best when the spermatozoa was diluted in TCG-6% egg yolk + 100 mm sucrose and warmed at 60°C. Slow warming at 37°C strongly reduced (p < .05) sperm motility and viability. However, sperm vitrification returned lower fertility, sperm motility and sperm viability values than conventional sperm freezing.

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Section: Discussioncontrasting

confidence: 47%

Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification

et al. 2016

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“…; Pradiee et al. ). Because the same volume of extender (1.5 ml) was used in both flushing and float‐out method, the final sperm concentration was related to the effectiveness of the method.…”

Section: Discussionmentioning

confidence: 98%

“…Resistance to low temperatures has been reported for the epididymal sperm of several mammalian species, including impala (Aepyceros melampus), lion (Panthera leo), bull (Bos taurus), buffalo (Syncerus caffer) and ibexes (Capra pyrenaica) (Gilmore et al 1998;Herold et al 2004;Muiño et al 2007;Pradiee et al 2014). Because the same volume of extender (1.5 ml) was used in both flushing and float-out method, the final sperm concentration was related to the effectiveness of the method.…”

Section: Discussionmentioning

confidence: 99%

Influence of Post‐Mortem Sperm Recovery Method and Extender on Unstored and Refrigerated Rooster Sperm Variables

Villaverde‐Morcillo¹,

Esteso²,

Castaño³

et al. 2015

Reprod Domestic Animals

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Many post-mortem sperm collection techniques have been described for mammalian species, but their use in birds is scarce. This paper compares the efficacy of two post-mortem sperm retrieval techniques - the flushing and float-out methods - in the collection of rooster sperm, in conjunction with the use of two extenders, i.e., L&R-84 medium and Lake 7.1 medium. To determine whether the protective effects of these extenders against refrigeration are different for post-mortem and ejaculated sperm, pooled ejaculated samples (procured via the massage technique) were also diluted in the above extenders. Post-mortem and ejaculated sperm variables were assessed immediately at room temperature (0 h), and after refrigeration at 5°C for 24 and 48 h. The flushing method retrieved more sperm than the float-out method (596.5 ± 75.4 million sperm vs 341.0 ± 87.6 million sperm; p < 0.05); indeed, the number retrieved by the former method was similar to that obtained by massage-induced ejaculation (630.3 ± 78.2 million sperm). For sperm collected by all methods, the L&R-84 medium provided an advantage in terms of sperm motility variables at 0 h. In the refrigerated sperm samples, however, the Lake 7.1 medium was associated with higher percentages of viable sperm, and had a greater protective effect (p < 0.05) with respect to most motility variables. In conclusion, the flushing method is recommended for collecting sperm from dead birds. If this sperm needs to be refrigerated at 5°C until analysis, Lake 7.1 medium is recommended as an extender.

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“…Following dissection of the epididymis, a cut is made close to the tail region, and the extender is then injected into the epididymal lumen and the content is expelled using a syringe filled with air. The sperm cells are then recovered (Monteiro et al, 2013;Pradiee et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders

Lima

Silva

Cortez

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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We aimed to compare fresh sperm and sperm cooled to 4ºC that had been recovered from the epididymides of cats using powdered coconut water (ACP-117c) and Tris extenders. Sixty epididymides were divided into 6 groups: 10 fresh epididymides were recovered using Tris (T0h); 10 were kept at 4°C/2h and recovered using Tris (T2h); 10 were kept at 4°C/4h and recovered using Tris (T4h); 10 fresh were recovered using ACP-117c (A0h); 10 were kept at 4°C/2h and recovered using ACP-117c (A2h), and 10 were kept at 4°C/4h and recovered using ACP-117c (A4h). The testis-epididymis complexes (TEC) control were not cooled. The others were cooled at 4°C for 2 or 4h. The epididymis was separated and the sperm was recovered by the modified flotation method. Sperm kinetic parameters were evaluated by a computer-system analysis, and vigor, viability, concentration, membrane function and morphology of the sperm were assessed under a light microscope. The progressive motility with ACP-117c declined after 2h of cooling, but did not differ between fresh and 4h. The vigor and membrane function were higher in A4h than A0h. The vigor at T2h and T4h were decreased compared to T0h. T0h was higher than A0h for vigor and sperm membrane function. However, after 4h of cooling, ACP-117c maintained a higher percentage of living cells. Feline epididymal sperm quality can be maintained to the degree necessary for artificial breeding programs following cooling and ACP-117c may be successfully used to recover cat sperm that have been cooled for up to 4h.

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Cryopreservation of epididymal sperm from ibexes (Capra pyrenaica) using short equilibration time with glycerol

Cited by 16 publications

References 22 publications

Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification

Conventional slow freezing cryopreserves mouflon spermatozoa better than vitrification

Influence of Post‐Mortem Sperm Recovery Method and Extender on Unstored and Refrigerated Rooster Sperm Variables

Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders

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