Cryopreservation of bison semen without exogenous protein in extender and its fertility potential in vitro and in vivo following fixed-time artificial insemination

Assisted reproductive techniques in wisents: Achievements and further challenges

“…Currently, in reproductive biotechnology, there is a growing tendency to avoid diluents containing animal products. Egg yolk or milk may be a potential source of pathogenic microorganisms, such as avian influenza virus, Salmonella sp., Escherichia coli or mycoplasmas (71). Elimination of potential pathogens from the gamete conservation process is important for creating stable genetic reserves and, in the future, for obtaining disease-free progeny (25,34,71).…”

“…Egg yolk or milk may be a potential source of pathogenic microorganisms, such as avian influenza virus, Salmonella sp., Escherichia coli or mycoplasmas (71). Elimination of potential pathogens from the gamete conservation process is important for creating stable genetic reserves and, in the future, for obtaining disease-free progeny (25,34,71). Currently, in research on the development of optimal extenders free of exogenous protein for various animal species, cyclodextrins are of a great interest (6,9,53,71).…”

“…Elimination of potential pathogens from the gamete conservation process is important for creating stable genetic reserves and, in the future, for obtaining disease-free progeny (25,34,71). Currently, in research on the development of optimal extenders free of exogenous protein for various animal species, cyclodextrins are of a great interest (6,9,53,71). Successful attempts to cryopreserve semen with media enriched with a complex of cyclodextrin and cholesterol were made in Bison bison athabascae, the effectiveness of which was confirmed in vitro and in vivo (71).…”

“…Currently, in research on the development of optimal extenders free of exogenous protein for various animal species, cyclodextrins are of a great interest (6,9,53,71). Successful attempts to cryopreserve semen with media enriched with a complex of cyclodextrin and cholesterol were made in Bison bison athabascae, the effectiveness of which was confirmed in vitro and in vivo (71). Considerable attention is also given to other semen additions, such as sericin (2), caffeine (61), melatonin (12) and antioxidants, such as vitamin C or polyphenols (70), which have not been tested in wisents.…”

“…The possibility of using the technique of AI in herds kept on farms has been demonstrated in the wood bison, closely related to the wisent (71). Yang et al (71) made an attempt to synchronize oestrus during the season by two methods: one was based on follicle ablation and the other involved using intravaginal steroid systems. In both groups, PGF2 and hCG were injected at different times after the administration of prostaglandin (71).…”