Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility

Yang, S. X.; Adams, Gregg P.; Zwiefelhofer, E. M.; Rajapaksha, Kosala; Anzar, M.

doi:10.1016/j.anireprosci.2021.106691

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…Excessive peroxidation can damage the sperm plasma membrane, leading to a decrease in sperm quality [ 30 ]. Cholesterol is the main component of the sperm plasma membrane, and a higher content of cholesterol in the plasma membrane makes it more resistant to cold shock [ 31 , 32 , 33 ]. Studies have shown that AMPK is involved in the maintenance of sperm plasma membrane, which can inhibit the process of inducing the membrane lipid disorder and reduce the translocation of phosphatidylserine to the outer surface of sperm [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Bai,

Zhang,

Shen

et al. 2023

Molecules

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The aim of this study was to investigate the effects of sodium salicylate (SS) on the preservation and metabolic regulation of sheep sperm. Under 4 °C low-temperature conditions, SS (at 10 µM, 20 µM, 30 µM, and 50 µM) was added to the semen diluent to detect sperm motility, plasma membrane, and acrosome integrity. Based on the selected optimal concentration of SS (20 µM), the effects of 20 µM of SS on sperms’ antioxidant capacity and mitochondrial membrane potential (MMP) were evaluated, and metabolomics analysis was conducted. The results showed that on the 20th day of low-temperature storage, the sperm motility of the 20 µM SS group was 62.80%, and the activities of catalase (CAT) and superoxide dismutase (SOD) were significantly higher than those of the control group (p < 0.01). The content of Ca2+, reactive oxygen species (ROS), and malondialdehyde (MDA) were significantly lower than those of the control group (p < 0.01), and the total antioxidant capacity (T-AOC) was significantly higher than that of the control group (p < 0.05); mitochondrial activity and the total cholesterol (TC) content were significantly higher than those in the control group (p < 0.01). An ultrastructural examination showed that in the SS group, the sperm plasma membrane and acrosome were intact, the fibrous sheath and axoneme morphology of the outer dense fibers were normal, and the mitochondria were arranged neatly. In the control group, there was significant swelling of the sperm plasma membrane, rupture of the acrosome, and vacuolization of mitochondria. Using metabolomics analysis, 20 of the most significant differential metabolic markers were screened, mainly involving 6 metabolic pathways, with the amino acid biosynthesis pathway being the most abundant. In summary, 20 µM of SS significantly improved the preservation quality of sheep sperm under low-temperature conditions of 4 °C.

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Section: Discussionmentioning

confidence: 99%

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Bai,

Zhang,

Shen

et al. 2023

Molecules

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“…Regrettably, extending the PGF to FTAI interval by an additional 24 h has led to a similar low pregnancy rate reported by Yapura (2013). Similarly, substituting a waxbased dip-coated letrozole device, used by Yang et al (2021), for a T-shaped silicone letrozole-releasing device has not improved the P/AI.…”

Section: Letrozole-based Protocolmentioning

confidence: 93%

Timed artificial insemination protocols in dairy cattle: Functioning, shortcomings, and improvements

Boudaoud¹

2023

CZECH J ANIM SCI

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Oestrus detection is currently at the top of the factors limiting the success of artificial insemination in dairy herds. This limitation becomes more pronounced as milk production increases because of higher metabolic clearance rates of steroid hormones leading to low circulating oestradiol concentration near oestrus. The standard OvSynch has overcome this problem by enabling fixed-time artificial insemination, for it synchronises follicular wave emergence, luteolysis, and ovulation. However, it did not improve fertility compared to cycles with spontaneous ovulation, which motivated the development of "fertility programs". These later are nothing but improved OvSynch protocol. This article explains what went wrong with the original OvSynch protocol, discusses the improvements made by "fertility programs", and highlights the advantages and limits of the newly tested molecules.

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“…Additionally, cooling-induced efflux of cholesterol from the membrane induces capacitation-like changes in frozen sperm [ 152 ]. Several articles have reported that the treatment of sperm before cryopreservation with cyclodextrins loaded with cholesterol leads to improved plasma membrane integrity and increases the osmotic tolerance of sperm [ 153 , 154 , 155 ].…”

Section: Prevention Of Sperm Cryoinjurymentioning

confidence: 99%

Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

Sharafi

Borghei-Rad

Hezavehei

et al. 2022

Animals

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Cryopreservation is a way to preserve germplasm with applications in agriculture, biotechnology, and conservation of endangered animals. Cryopreservation has been available for over a century, yet, using current methods, only around 50% of spermatozoa retain their viability after cryopreservation. This loss is associated with damage to different sperm components including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, and microRNAs. To mitigate this damage, conventional strategies use chemical additives that include classical cryoprotectants such as glycerol, as well as antioxidants, fatty acids, sugars, amino acids, and membrane stabilizers. However, clearly current protocols do not prevent all damage. This may be due to the imperfect function of antioxidants and the probable conversion of media components to more toxic forms during cryopreservation.

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Cholesterol-cyclodextrin complex as a replacement for egg yolk in bull semen extender: sperm characteristics post-thawing and in vivo fertility

Cited by 4 publications

References 39 publications

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Metabolomics Analysis of Sodium Salicylate Improving the Preservation Quality of Ram Sperm

Timed artificial insemination protocols in dairy cattle: Functioning, shortcomings, and improvements

Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

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