Cryopreservation of Atlantic croaker spermatozoa

Gwo, Jin‐Chywan; Strawn, Kirk; Longnecker, Michael T.; Arnold, C. R.

doi:10.1016/0044-8486(91)90179-b

Cited by 84 publications

(47 citation statements)

References 50 publications

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“…This fact was vouched by Cognie et al (1989) who found that the motility and fertility of deep frozen spermatozoa of C. carpio were significantly improved when the dilution ratio was reduced from 1:100 to 1:2. According to Rana and Mc Andrew (1989) and Gwo et al (1991) the milt dilution ratio had strong effect on fertility. There was significant difference (p˂0.05) between the fertilisation and hatching rates of different age group brooders and between different dilution ratios.…”

Section: Discussionmentioning

confidence: 99%

Captive broodstock development of common carp Cyprinus carpio Linnaeus, 1758: influence of age on spermatogenesis and fertilisation parameters

Betsy¹,

Kumar²,

Jawahar³

et al. 2016

Indian J. Fish.

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Knowledge on the quality of male and female gametes which may affect fertilisation success and larval survival is necessary to enhance fish production to meet global demand. Gamete quality is affected by factors like sex ratio, stocking density, age, size, nutrition and feeding regime. In the present study, the spermatological and fertilisation parameters as influenced by the age of brooders of Cyprinus carpio Linnaeus 1758 developed under captive condition from a single population were studied with special reference to dilution ratios. Milt was collected from 6, 12 and 18 months old brooders of C. carpio and diluted at three ratios viz., 1:40, 1:80 and 1:120 using freshwater fish saline. Sperm density, spermatocrit value, seminal plasma composition, motility duration, fecundity, fertilisation and hatching rates were studied for brooders of all age groups. The results showed that milt from 12 months old brooder produced good quality milt in terms of motility duration (69.66±4.72 sec), fertilisation rate (91.3±3.05%) and hatching rate (76.5±2.64%) at 1:40 dilution ratio.

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Section: Discussionmentioning

confidence: 99%

Captive broodstock development of common carp Cyprinus carpio Linnaeus, 1758: influence of age on spermatogenesis and fertilisation parameters

Betsy¹,

Kumar²,

Jawahar³

et al. 2016

Indian J. Fish.

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“…Most subsequent studies, however, have been of freshwater species, especially salmonids (see reviews by Scott Cryopreservation of sperm from the Atlantic croaker (Micropogonius undulutus) was studied by Gwo et al (1991) and represents the only report on cryopresemation of sperm f " a sciaenid species.…”

Section: Thefamilysciaenidaecontajnsseveralspeciesimportantmentioning

confidence: 99%

“…The cryopmtectant yielding highest post-thaw motility was DMSO at 10% wben a freezing rate of -35"cImin was used, although faster rates of freezing have been shown to be successful in the cryopreservation of Atlantic croaker sperm (Gwo et al 1991). Sperm frozen in the computer-controlled freezerat45"C/minretainedalowerpost-thawmotility than sperm hzenat either dewar freezing rate.…”

mentioning

confidence: 99%

Cryopreservation of Sperm of Spotted Seatrout (Cynoscion nebulosus)

Wayman

Thomas

Tiersch

1996

GCR

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Cryopreservation of fish sperm has applications in preserving genetic resources from stocks of endangered fishes, replenishing fisheries, reducing the number of males needed in hatchery situations, and allowing repeated spawning of specific males. As part of a larger study on artificial breeding of sciaenid fishes, we developed procedures for collection, handling, refrigerated storage, and cryopreservation of spotted seatrout sperm. Hanks' balanced salt solution (HBSS) was used as an extender for collection and storage of sperm. Sperm motility in relation to graded concentrations of HBSS was used to determine the osmolality at which sperm were activated. Based on these findings, HBSS was prepared at 201 mOsm/kg as an extender for sperm storage. To determine if ions present in HBSS were involved in sperm activation, separate activating solutions were prepared by the addition of NaC1, CaCJ, KC1, Na,HFQ,, or MgSO, to aliquots of a stock glucose solution (185 mOsm/kg).The chemicals were added at the concentration of each found in 1-x HBSS. Only the glucose solution containing 8 g/l NaCl(424 mOsm/kg) produced activation of sperm. We also evaluated four chemicals as cyroprotectants: methanol, glycerol, dimethyl sulfoxide (DMSO), and n,n-dimethyl acetamide. Two freezing rates were evaluated by placing samples at either of two heights within a nitrogen vapor shipping dewar. The highest post-thaw motilities were in 10% DMSO with an average retention of 60% of initial motility at the lower position in the dewar, and 37% at the upper position. A third freezing rate was produced using a computer-controlled freezer programmed for a rate of -45"C/min, yielding a retention of initial motility of 31%. Our freezing and transport of cryopreserved sperm in shipping dewars demonstrate the utility of this procedure for field applications.

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“…Tiersch (2000) has also reported that the application of cryoprotectant at concentration between 5% to 20% ususally provides good protection in most fish species. The protective effect and optimal concentration of cryoprotectants could be species specific (Gwo et al, 1991;Suquet et al, 2000;Rideout et al, 2003). Therefore, the optimal concentration needs to be determined individually in each species studied through experimentations.…”

Section: Dilution Of Semen Samplementioning

confidence: 99%