Cryoprecipitate: An autologous substrate for human fetal retinal pigment epithelium

Farrokh-Siar, Lili; Ka, Rezai; Sc, Patel; Ernest, J. Terry

doi:10.1076/ceyr.19.2.89.5331

Cited by 29 publications

(15 citation statements)

References 13 publications

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“…As the authors gave no specific information, an estimation of the thickness on a photograph suggests a thickness of about 1,000 μm. This thickness may allow an easy extraocular handling as described by the authors but may pose a problem to the overlying retina and RPE when transplanted [5]. Castellarin et al describe the use of cadaver Bruch's membrane as scaffolding for fetal RPE.…”

Section: Discussionmentioning

confidence: 94%

“…Transplanted RPE cells have been shown to deteriorate within 24 hours, if attachment to Bruch's membrane has not occurred [4]. Several studies have therefore investigated different substrates and cells such as cryoprecipitated extracellular matrix membranes [5], anterior lens capsule [6], cadaver Bruch's membrane [7], Descemet's membrane [8], synthetic biodegradable polymer films [9], collagen type I [10], microspheres of cross-linked fibrinogen [11], non-degradable polymer substrates [3] and amniotic membrane [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inner limiting membrane as membranous support in RPE sheet-transplantation

Beutel

Greulich

Lüke

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Inner limiting membrane as membranous support in RPE sheet-transplantation

Beutel

Greulich

Lüke

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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“…Cryoprecipitate, a substance composed primarily of fibrinogen and fibronectin [138] can be processed into a membrane [138]. These membranes have the potential to be an autologous scaffold, with material developed from a patient′s own blood.…”

Section: Scaffolds For Retinal Pigment Epithelium Cell Transplantationmentioning

confidence: 99%

“…An interesting approach has been the production of cryoprecipitate from blood plasma [138], and ECM from RPE cells [139]; both produced by freeze-thaw cycles. Cryoprecipitate, a substance composed primarily of fibrinogen and fibronectin [138] can be processed into a membrane [138].…”

Section: Scaffolds For Retinal Pigment Epithelium Cell Transplantationmentioning

confidence: 99%

A tissue-engineered approach towards retinal repair: Scaffolds for cell transplantation to the subretinal space

Hynes

Lavik

2010

Graefes Arch Clin Exp Ophthalmol

159

135

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“…To achieve this it is necessary to identify the optimal substrates on which to grow the cells in culture prior to implantation. To date a number of substrates have been studied and cells have been grown on and transplanted on substrates such as cryoprecipitated membranes [12], anterior lens capsule [23, 31, 53], cadaver Bruch's membrane [7], Descemet's membrane [78], synthetic biodegradable polymer films [15], collagen type I [5] and as microspheres on crosslinked fibrinogen [54]. Most of this research concentrates on the use of either biological substrates or degradable substrates to sustain the RPE monolayer.…”

Section: Underlying Substratesmentioning

confidence: 99%

Basement membranes and artificial substrates in cell transplantation

Sheridan

Williams

Grierson

2003

Graefe's Arch Clin Exp Ophthalmol

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Basement membranes and artificial substrates in cell transplantationchange influenced by both genetic predisposition and environmental factors and focused at the level of Bruch's membrane. A number of age-related changes occur at the level of Bruch's membrane [26, 32]. Morphological and biochemical studies have demonstrated that the changes that occur to Bruch's membrane are typified by increased thickness and the progressive accumulation of deposits (such as drusen-see later) within the inner layers of the membrane.Bruch's membrane is a basement membrane complex located between the retinal pigment epithelium (RPE) and the choroid. It is a pentalaminar structure with a central elastin layer bordered on either side by a collagenous zone. There is the basal lamina of the RPE at the innermost collagenous layer of Bruch's membrane and a second basal lamina, produced by the endothelial cells of the choriocapillaris, that is juxtaposed to the outer collagenous layer. Although much more remains to be learned, the basic ultrastructural organization and biochemical composition of Bruch's membrane are similar to basement membrane complexes in other tissues, such as the choroid plexus, kidney glomerulus, and airway alveoli, where an epithelial-endothelial juxtaposition occurs. All ionic exchange and metabolic traffic from the neural retina and RPE to the choroidal capillaries, and vice versa, must traverse Bruch's membrane, thus leaving the neural retina vulnerable to any disruptions of those processes [3]. It is well documented that Bruch's membrane undergoes a number of changes throughout life, including increased thickening, protein cross-linking and reduced permeability to nutrients as well as increased amounts of lipid deposition and the accumulation of basal laminar deposits and drusen (for more detailed reviews see [13, 22,86].Drusen are insoluble deposits that accumulate at the interface between Bruch's membrane and the RPE. Clinically, drusen are divided into two main morphologic phenotypes-hard and soft. Hard drusen are hemispherical structures with well-defined edges, whereas soft This article will concentrate largely on the current developments in the area of cell transplantations presented at the 1st Workshop for Cell Transplantation in Age-related Macular Degeneration. In particular, this brief review will address our current understanding of the role of cell-matrix interactions by covering the pathobiology of normal ageing Bruch's membrane; some of the problems faced at the time of surgery from a basement membrane prospective; the dedifferentiation and differentiation of RPE cells; and how the use of artificial substrates may address several of these issues. We will concentrate on problems related to age-related macular degeneration (AMD), the leading cause of irreversible blindness in Europe, America and other industrialized nations. AMD is likely to be a family of disorders, rather than a single biologic entity, characterized by the progressive loss of sight in the central portion of the visual field [6, 56...

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Cryoprecipitate: An autologous substrate for human fetal retinal pigment epithelium

Abstract: Cryoprecipitate membranes may provide an ideal source for the adhesion, cultivation, and transfer of HFRPE cells. Their autologous isolation from the recipient's blood grants an additional advantage for their application as a carrier for HFRPE transplantation into the subretinal space.

Cited by 29 publications

References 13 publications

Inner limiting membrane as membranous support in RPE sheet-transplantation

Inner limiting membrane as membranous support in RPE sheet-transplantation

A tissue-engineered approach towards retinal repair: Scaffolds for cell transplantation to the subretinal space

Basement membranes and artificial substrates in cell transplantation

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