A tissue-engineered approach towards retinal repair: Scaffolds for cell transplantation to the subretinal space

Hynes, Sara Royce; Lavik, Erin B.

doi:10.1007/s00417-009-1263-7

Cited by 159 publications

(139 citation statements)

References 148 publications

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“…Transplantation of neural stem or precursor cells has been used as a reliable therapeutic strategy in restoring tissue damage and regaining lost function in the CNS [1][2][3][4]. Direct cell transplantation, however, suffers several disadvantages such as limited survival upon implant ation and the formation of abnormal cell architecture in vivo [5]. In recent years, biomimetic 3D scaffolds have emerged as a very encouraging method for repairing a damaged nervous system including both spinal cord and peripheral nerve injuries.…”

Section: Introductionmentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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Objective. Nanomaterials, such as carbon nanotubes (CNTs), have been introduced to modify the surface properties of scaffolds, thus enhancing the interaction between the neural cells and biomaterials. In addition to superior electrical conductivity, CNTs can provide nanoscale structures similar to those present in the natural neural environment. The primary objective of this study is to investigate the proliferative capability and differential potential of neural stem cells (NSCs) seeded on a CNT incorporated scaffold. Approach. Amine functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated with a PEGDA polymer to provide enhanced electrical properties as well as nanofeatures on the surface of the scaffold. A stereolithography 3D printer was employed to fabricate a well-dispersed MWCNT-hydrogel composite neural scaffold with a tunable porous structure. 3D printing allows easy fabrication of complex 3D scaffolds with extremely intricate microarchitectures and controlled porosity. Main results. Our results showed that MWCNT-incorporated scaffolds promoted neural stem cell proliferation and early neuronal differentiation when compared to those scaffolds without the MWCNTs. Furthermore, biphasic pulse stimulation with 500 µA current promoted neuronal maturity quantified through protein expression analysis by quantitative polymerase chain reaction. Significance. Results of this study demonstrated that an electroconductive MWCNT scaffold, coupled with electrical stimulation, may have a synergistic effect on promoting neurite outgrowth for therapeutic application in nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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“…86 Natural materials provide the advantage of directing cell behavior by closely imitating the native extracellular matrix (ECM) of the targeted tissue, although they raise concerns such as the purity of animal-derived scaffold, risk of infection, difficulties controlling consistency of the material, and mechanical instability. 87,88 In contrast, synthetic scaffolds can be custom made, including incorporation of biocompatibility, mimicking native ECM, and biodegradability. 89 Poly lactic-co-glycolic acid is one of the most widely used synthetic polymers.…”

Section: Scaffold Material Fabrication Technique and Propertiesmentioning

confidence: 99%

Induced Pluripotent Stem Cell Therapies for Degenerative Disease of the Outer Retina: Disease Modeling and Cell Replacement

Foggia

Makwana

Ali³

et al. 2016

Journal of Ocular Pharmacology and Therapeutics

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Stem cell therapies are being explored as potential treatments for retinal disease. How to replace neurons in a degenerated retina presents a continued challenge for the regenerative medicine field that, if achieved, could restore sight. The major issues are: (i) the source and availability of donor cells for transplantation; (ii) the differentiation of stem cells into the required retinal cells; and (iii) the delivery, integration, functionality, and survival of new cells in the host neural network. This review considers the use of induced pluripotent stem cells (iPSC), currently under intense investigation, as a platform for cell transplantation therapy. Moreover, patientspecific iPSC are being developed for autologous cell transplantation and as a tool for modeling specific retinal diseases, testing gene therapies, and drug screening.

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“…Tissue engineering can be defined as using a polymer scaffold with the appropriate architecture to guide the repair and restoration of function to damaged tissues or organs [17]. The field was largely developed in order to address the shortage of tissues and organs available for transplantation [18].…”

Section: Polymer Scaffoldsmentioning

confidence: 99%

Synthetic Polymer Scaffolds for Stem Cell Transplantation in Retinal Tissue Engineering

2011

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Age-related macular degeneration and retinitis pigmentosa are two leading causes of irreversible blindness characterized by photoreceptor loss. Cell transplantation may be one of the most promising approaches of retinal repair. However, several problems hinder the success of retinal regeneration, including cell delivery and survival, limited cell integration and incomplete cell differentiation. Recent studies show that polymer scaffolds can address these three problems. This article reviews the current literature on synthetic polymer scaffolds used for stem cell transplantation, especially retinal progenitor cells. The advantages and disadvantages of different polymer scaffolds, the role of different surface modifications on cell attachment and differentiation, and controlled drug delivery are discussed. The development of material and surface modification techniques is vital in making cell transplantation a clinical success.

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A tissue-engineered approach towards retinal repair: Scaffolds for cell transplantation to the subretinal space

Cited by 159 publications

References 148 publications

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

Induced Pluripotent Stem Cell Therapies for Degenerative Disease of the Outer Retina: Disease Modeling and Cell Replacement

Synthetic Polymer Scaffolds for Stem Cell Transplantation in Retinal Tissue Engineering

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