Cryobanking as tool for conservation of biodiversity: Effect of brown trout sperm cryopreservation on the male genetic potential

Martínez‐Páramo, S.; Pérez‐Cerezales, Serafín; Gómez-Romano, Fernando; Blanco, Gloria; Sánchez, José A.; Herráez, M.P.

doi:10.1016/j.theriogenology.2008.09.034

Cited by 70 publications

(46 citation statements)

References 40 publications

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“…These findings are crucial for planning efficient artificial fertilization design in restoration programs involving artificial breeding and other innovative conservation strategies such as gamete cryopreservation, considered an effective method to save endangered fish species by the storage of their gametes in gene banks (Gausen, 1993;Akcay et al, 2004;Martínez-Páramo et al, 2009;Iaffaldano et al, in press) and the utilization in crossed fertilization protocols.…”

Section: Discussionmentioning

confidence: 99%

“…After 10 min of incubation at room temperature in the dark, 3 μL of working PI solution (PI solution diluted 1:100 in phosphate buffered saline "PBS" diluent), was added to the cell suspension. Spermatozoa were incubated for a further 10 min under the same conditions (Martínez-Páramo et al, 2009). Next, 10 μL of this suspension were placed on microscope slides, covered with coverslips and examined at a magnification ×1000 using a ×100 oil immersion objective under epifluorescence illumination.…”

Section: Evaluation Of Sperm Qualitymentioning

confidence: 99%

“…SYBR-14, a membrane permeable nucleic acid stain, emits green fluorescence, whereas PI, a nonpermeable probe, emits red fluorescence (Martínez-Páramo et al, 2009).…”

Section: Evaluation Of Sperm Qualitymentioning

confidence: 99%

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Iaffaldano¹,

Iorio²,

Manchisi³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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This research aimed to provide a characterization of fresh semen in wild and threatened Mediterranean brown trout (Salmo cettii), inhabiting the Biferno River basin and to study the variance of sperm parameters in a reproductive system with high male competition. The evaluated sperm quality parameters were: volume, density, viability, motility and sperm movement duration. High variability in sperm traits were found probably in response to the heterogeneity of individual condition, social status, spawning activity and migration costs. Duration of motility was positively correlated (P<0.05) with age class of breeders and motility. Stronger positive correlations (P<0.001) were observed between motility and viability and between sperm counts and viable sperms. This study reported the first data about semen quality of an Italian wild population of native trout. These results are useful for planning efficient artificial fertilization protocols in restoration programs involving supportive breeding together with other innovative conservation strategies as gamete cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Sperm Qualitymentioning

confidence: 99%

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Iaffaldano¹,

Iorio²,

Manchisi³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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“…Cryopreservation of fish sperm is relatively common in the breeding and management of fish species, including salmonid, cyprinids, silurids, sturgeons [29][30][31][32].The creation of a cryopreserved sperm bank is an effective strategy for protecting the biodiversity of fish population and provides the opportunity to preserve the sperm samples of the most valuable males, which can be used in reproductive technologies in hatchery conditions. Sperm cryobanking could be a real alternative to breeding in captivity in order to preserve genetic diversity [33]. Sperm cryopreservation is a valuable conservation tool with applications in genome security as well as wider practical uses in minimizing the potential risk of disease transmission and removing animal welfare concerns associated with transporting live animals between breeding facilities [34].…”

Section: Sperm Preservationmentioning

confidence: 99%

Bio-banking: An Emerging Approach for Conservation of Fish Germplasm

Goswami¹,

Mishra²,

Ns³

et al. 2016

Poult Fish Wildl Sci

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Global biodiversity is declining with substantial losses of populations, species and habitats causing a significant impact on human life. Holistic and integrated approach for biodiversity conservation requires concrete and urgent action. Although there is increasing concern over the loss of species and degradation of habitat, still more innovative efforts are required. Biobanking holds immense promise to provide biomaterial for conservation of fish germplasm. Establishment of repositories along with gene banks could provide opportunities for collaboration among the leading experts in the fields of fish ecology, physiology, and cryobiology to synthesize effective ways for conservation of fish genetic resources, and discuss what needs to be done to increase the impact in the next century. The article reviews different causes for loss of fish biodiversity, the ways to overcome the situation and advocates cell line repository as an alternative aid to conserve fish germplasm.

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“…In addition to being indicated to minimize asynchrony in the maturation of gametes and facilitate their transport, this technique limits the stock of males in intensive fish farming, reducing production costs (MARTÍNEZ-PÁRAMO et al, 2009). Cryopreservation is also important to preserve diversity of native species, reducing the negative impacts of the hydroelectric dams in the reproductive cycle.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Prochilodus lineatus semen: effect of cryoprotectants combination

et al. 2015

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The study was conducted with the objective of comparing the toxicity and the effect of the combination of intra and extracellular cryoprotectants in curimba Prochilodus lineatus sperm cells subjected to cryopreservation. Semen from 19 males were analyzed and diluted in four solutions comprise of intra and extracellular cryoprotectants in the following combinations: methanol+lactose, methanol+egg yolk, DMSO+lactose and DMSO+egg yolk. A portion of the diluted semen was frozen while the remaining fraction was kept in repose and evaluated after 10 min. For freezing, the diluted samples were packaged in 0.50 mL straws and placed into liquid nitrogen vapor for 24 h and, after this time, submerged into liquid nitrogen for 10 days. The combination of DMSO+lactose was less toxic to the diluted semen, resulting in higher motility rates and durations when compared to the other treatments. After thawing, the highest motility rate and duration were obtained using lactose as extracellular cryoprotectant, regardless of its combination. There was no significant difference between treatments when analyzing sperm morphology after thawing. Considering the effects of the tested treatments, the use of lactose as an extracellular cryoprotectant added with DMSO or methanol is the most suitable, since these combinations presented the highest motility rates and durations and low morphological change rate after thawing.Keywords: genetic preservation; cryobiology; sperm analysis; freezing protocol; toxicity; curimba CRIOPRESERVAÇÃO DO SÊMEN DO Prochilodus lineatus: EFEITO DA COMBINAÇÃO DE CRIOPROTETORES RESUMOO estudo foi realizado com o objetivo de comparar a toxicidade e o efeito da combinação de crioprotetoresintra e extracelulares em espermatozóides de curimba Prochilodus lineatus submetidos à criopreservação. O sêmen de 19 machos foi analisado e diluído em quatro soluções com crioprotetores intra e extracelulares nas seguintes combinações: metanol+lactose, metanol+gema de ovo, DMSO+lactose e DMSO+gema de ovo. Uma porção do sêmen diluído foi congelada enquanto que a fração remanescente foi mantida em repouso e avaliada após 10 min. Para o congelamento, as amostras diluídas foram envasadas em palhetas de 0,50 mL e colocadas em vapor de nitrogênio líquido durante 24 h e, após este tempo, submersas em nitrogênio líquido durante 10 dias. A combinação de DMSO+lactose se mostrou menos tóxica para o sêmen diluído, resultando em taxas mais elevadas de motilidade e duração espermática quando comparadas com os outros tratamentos. Após descongelamento, as maiores taxas de motilidade e duração foram obtidas usando lactose como crioprotector extracelular, independentemente da sua combinação. Não houve diferença significativa entre os tratamentos ao analisar a morfologia espermática após a descongemento. Considerando-se os efeitos dos tratamentos utilizados, o uso de lactose como crioprotector extracelular, adicionada ao DMSO ou metanol, é o mais adequado, uma vez que estas combinações apresentaram maiores taxas de motilidade e duração espe...

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Cryobanking as tool for conservation of biodiversity: Effect of brown trout sperm cryopreservation on the male genetic potential

Cited by 70 publications

References 40 publications

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Bio-banking: An Emerging Approach for Conservation of Fish Germplasm

Cryopreservation of Prochilodus lineatus semen: effect of cryoprotectants combination

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