Crude Xylanases and Pectinases from Bacillus spp. Along with Commercial Cellulase Formulate an Efficient Tailor-Made Cocktail for Sugarcane Bagasse Saccharification

Thite, Vihang S.; Nerurkar, Anuradha S.

doi:10.1007/s12155-019-10050-5

Cited by 15 publications

(9 citation statements)

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“…In the enzymatic hydrolysis process, cellulase acts synergistically with other enzymes in the hydrolysis of cellulose ( Borges et al, 2014 ). Although PK did not contain an appreciable quantity of pectin, pectinolytic preparations were also incorporated in enzyme cocktails containing cellulase, as Thite and Nerurkar (2020) reported about the synergistic action of pectinases, xylanases, and cellulases on SB, which also have very low levels of pectin. Pectinases served as accessory enzymes, and ∼1.6- to ∼1.9-fold increases in agro-waste biomass saccharification were obtained by using enzyme cocktail of cellulases, xylanases, and pectinases ( Thite and Nerurkar, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail

et al. 2021

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Section: Resultsmentioning

confidence: 99%

Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail

et al. 2021

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“…También las industrias de biocombustibles demandan esta enzima junto con las xilanasas, pues mejorar la sacarificación de la biomasa vegetal, durante la producción de biocombustible (Thite & Nerurkar, 2019).…”

Section: Resultados Y Discusión Tinción De Gram Y Morfología Bacterianaunclassified

Bacteria associated with Sarcocornia neei (Lag.) With apparent hydrolytic activity and biological control from the Paraguayan dry Chaco

2021

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Resumen: Las plantas halófitas muestran un nicho perfecto para aislar bacterias de interés, pues están adaptadas a ambientes extremos, estas bacterias podrían ser analizadas y explotadas por sus capacidades enzimáticas en la investigación y en diversas industrias. En este trabajo analizamos la actividad enzimática de las bacterias endofíticas y la rizosfera de Sarcocornia neei, una halófita que se desarrolla en los saladares chaqueños. La actividad enzimática se determinó mediante placas de Petri, que contenían medio base, suplementado con 2 g / l (almidón, carboximetilcelulosa, pectina y caseína). Se determinó degradación enzimática por la formación de halos. Se obtuvieron un total de 83 aislamientos bacterianos, siendo Gram positivas 80 (96,3%) y Gram negativas 3 (3,6%). En los ensayos cualitativos de producción de enzimas hidrolíticas, se determinaron productores de amilasas 50,6%, celulasas 40,9%, pectinasas 36,1% y proteasas 10,8%. Los aislados bacterianos capaces de producir enzimas hidrolíticas fueron mayoritariamente bacterias endofíticas, de los cuales siete aislados fueron capaces de producir simultáneamente amilasas, celulasas y pectinasas.Finalmente, las bacterias capaces de inhibir el crecimiento de F. solani fueron 13,8%, mientras que el 4,6% de los aislados fueron capaces inhibir el crecimiento de K. pneumoniae y solo el 0,6% mostró actividad antimicrobiana frente a S. aureus.

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“…For instance, the thermostability of the endoglucanase Cel8A [ 95 , 212 , 213 ], exoglucanase Cel48S [ 214 ] and β Glucosidase A [ 96 ] from Clostridium thermocellum increased upto14-fold when compared with the wild type strain with increased yield in soluble form of glucose using these methods. The substitutive approach for tailor-made cocktails formulations of xylanses and pectinase increased saccharification in bagasse suggesting another strategy for optimization of CAZymes for industrial application [ 215 ]. Almost all the commercially available cellulase from dominating enzyme suppliers are the products of random mutagenesis generated derivative of mutant strain RUT-30 of Trichoderma reesei which has excessive cellulase producing properties [ 216 ].…”

Section: Cazyme Application In Biorefineriesmentioning

confidence: 99%

Innovations in CAZyme gene diversity and its modification for biorefinery applications

Chettri

Verma

2020

Biotechnology Reports

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Highlights The review analyses the role of CAZymes in lignocellulose degradation with focus on the different approaches for improving the enzyme activity. Omics based approaches for the analysis of genes, transcripts, proteins and metabolites related to plant degrading mechanism. Directed evolution and rational design as approaches for engineering to enhancing the CAZyme properties. Microbial engineering of the fungal and microbial strains by adapting techniques like CRISPR/cas9 and use of various expression systems. Novel enzymes and cocktail formulation for biorefinery-based application for production of value-added products using low cost plant biomass.

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Crude Xylanases and Pectinases from Bacillus spp. Along with Commercial Cellulase Formulate an Efficient Tailor-Made Cocktail for Sugarcane Bagasse Saccharification

Cited by 15 publications

References 53 publications

Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail

Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail

Bacteria associated with Sarcocornia neei (Lag.) With apparent hydrolytic activity and biological control from the Paraguayan dry Chaco

Innovations in CAZyme gene diversity and its modification for biorefinery applications

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