Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail

Ansari, Immad; Ejaz, Uroosa; Abideen, Zainul; Gulzar, Salman; Syed, Muhammad Noman; Liu, Jing; Li, Wang; Fu, Pengcheng; Sohail, Muhammad

doi:10.3389/fmicb.2021.714940

Cited by 12 publications

(1 citation statement)

References 65 publications

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“…Of all the pre-treatment methods used, the substrate subjected to pre-treatment using acid in an autoclave, was found to give maximum yield of reducing sugar upon enzymatic hydrolysis. The results were also consistent with Ansari et al (2021) , who screened different halophytes for enzymatic hydrolysis. Results of saccharification revealed that acid pre-treated P. karka yielded maximum monosaccharides.…”

Section: Resultssupporting

confidence: 89%

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases

Nawaz

Qadoos²,

Haq³

et al. 2023

Front. Bioeng. Biotechnol.

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Bioethanol is believed to be an influential revolutionary gift of biotechnology, owing to its elevating global demand and massive production. Pakistan is home to a rich diversity of halophytic flora, convertible into bounteous volumes of bioethanol. On the other hand, the accessibility to the cellulosic part of biomass is a major bottleneck in the successful application of biorefinery processes. The most common pre-treatment procedures existent include physicochemical and chemical approaches, which are not environmentally benign. To overcome these problems, biological pre-treatment has gained importance but the drawback is the low yield of the extracted monosaccharides. The current research was aimed at exploring the best pre-treatment method for the bioconversion of halophyte Atriplex crassifolia into saccharides using three thermostable cellulases. Atriplex crassifolia was subjected to acid, alkali and microwave pre-treatments, followed by compositional analysis of the pre-treated substrates. Maximum delignification i.e. 56.6% was observed in the substrate pre-treated using 3% HCl. Enzymatic saccharification using thermostable cellulases also validated the results where the highest saccharification yield i.e. 39.5% was observed for the sample pre-treated using same. Maximum enzymatic hydrolysis of 52.7% was obtained for 0.40 g of the pre-treated halophyte Atriplex crassifolia where Endo-1,4-β-glucanase (300U), Exo-1,4-β-glucanase (400U) and β-1,4-glucosidase (1000U) were simultaneously added and incubated for 6 h at 75°C. The reducing sugar slurry obtained after optimization of saccharification was utilized as glucose in submerged fermentation for bioethanol production. The fermentation medium was inoculated with Saccharomyces cerevisiae, incubated at 30°C and 180 rpm for 96 h. Ethanol production was estimated using potassium dichromate method. Maximum production of bioethanol i.e. 16.33% was noted at 72 h. It can be concluded from the study that Atriplex crassifolia owing to its high cellulosic content after pre-treatment using dilute acid method, yields substantial amount of reducing sugars and high saccharification rates when subjected to enzymatic hydrolysis using thermostable cellulases, under optimized reaction conditions. Hence, the halophyte Atriplex crassifolia is a beneficial substrate that can be utilized to extract fermentable saccharides for bioethanol production.

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Section: Resultssupporting

confidence: 89%