Crude Tissue Culture Antigen for Determination of Varicella-Zoster Complement Fixing Antibody

Brunell, Philip A.; Casey, Helen

doi:10.2307/4592257

Cited by 54 publications

(11 citation statements)

References 7 publications

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“…First, we analyzed the RFLPs of ORF38 ( Pst I), ORF54 ( Bgl I), and ORF62 ( Sma I) as previously described [6, 8, 9]. The strain Ellen [17, 18] and the strain Kawaguchi [19] were used to represent the American laboratory VZV and the Japanese wild-type VZV, respectively, as controls. Most VZV isolates in Europe and America, including strain Dumas [20], strain MLS [21], and strain Ellen, contain a Pst I restriction enzyme site in ORF38 (positive for the Pst I site: Pst I + ).…”

Section: Case Presentationmentioning

confidence: 99%

Genetic analysis of varicella-zoster virus in the aqueous humor in uveitis with severe hyphema

et al. 2017

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BackgroundGenetic variations have been identified in the genome of varicella-zoster virus (VZV) strains using vesicle fluid, varicella scabs and throat swab samples. We report a rare case of VZV-associated uveitis with severe hyphema, which was immediately diagnosed by polymerase chain reaction (PCR) using the aqueous humor, in which we were able to analyze the VZV genotype for the first time.Case presentationA 16-year-old Japanese boy was referred to our hospital with a 20-day history of unilateral anterior uveitis and 11-day history of hyphema. At presentation, details of the iris, the iridocorneal angle, and the fundus were not visible due to the severe hyphema. Serum anti-VZV IgG and anti-VZV IgM were elevated, and 1.61 × 109 copies/mL of VZV-DNA were detected by real-time PCR using the aqueous humor. As there were no eruptions on his face or body, we diagnosed zoster sine herpete and started intravenous administration of prednisolone and acyclovir. The hyphema completely disappeared 2 weeks after presentation, while sectorial iris atrophy and mild periphlebitis of the fundus became gradually apparent. Anterior inflammation and periphlebitis gradually improved and VZV-DNA in the aqueous humor was reduced to 1.02 × 106 copies/mL at 4 weeks after presentation. Examination by slit lamp microscope revealed no inflammation after 5 months, and VZV-DNA could no longer be detected in the aqueous humor. Serum anti-VZV IgG and anti-VZV IgM also showed a gradual decrease along with improvement in ocular inflammation. The genetic analysis of multiple open reading frames and the R5 variable repeat region in the VZV genes, using DNA extracted from the aqueous humor at presentation, showed that the isolate was a wild-type clade 2 VZV strain (prevalent in Japan and surrounding countries) with R5A allele and one SNP unique to clade 1 (both are major types in Europe and North America).ConclusionsVZV-associated uveitis may develop hyphema that obscures ocular inflammation, thus PCR analysis using the aqueous humor is the key investigation necessary for the diagnosis. The measurement of VZV-DNA copies by real-time PCR would be useful for evaluation of therapeutic effects. We could amplify and analyze VZV genotype using the aqueous humor including a very large number of VZV-DNA copies (1.61 × 109 copies/mL).

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Section: Case Presentationmentioning

confidence: 99%

Genetic analysis of varicella-zoster virus in the aqueous humor in uveitis with severe hyphema

et al. 2017

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“…According to the report of Weller and Witton (1958) CF antibody against v-z virus became detectable within 4-5 days after clinical onset of the disease and reached a peak by 2 weeks, Brunell and Helen (1964) said that CF antibody titer against v-z virus began to decline by 2-3 months after onset and was able to detect during one year. According to the report of Taylor-Robinson (1959) neutralizing antibody titers against v-z virus were able to be detected over a few years.…”

Section: Methodsmentioning

confidence: 99%

Serological Studies on Two Epidemics of Varicella in a Babies Home

Ikeda

Agatsuma

Wataya

et al. 1973

Tohoku J. Exp. Med.

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“…Quantitative immunoglobulins were determined during the acute illness by radial immunodiffusion (12 (12). Anti-VZ CFA titrations were performed by the method of Brunell and Casey (13). Vesicle fluid interferon (V-IF) titrations, and studies to determine if the interfering activity had the physicochemical properties of interferon, were performed as described previously (14).…”

Section: Methodsmentioning

confidence: 99%

Interferon, antibody, and other host factors in herpes zoster

Stevens¹,

Merigan²

1972

J. Clin. Invest.

102

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A B S T R A C T The influence of several factors on the course of herpes zoster was studied in 151 patients. Dissemination of zoster was associated with the presence of a concurrent disease, especially Hodgkin's disease, and/or the use of immunosuppressive therapy. Several host-immune parameters, including quantitative immunoglobulins, circulating lymphocyte counts, delayed hypersensitivity to multiple skin test antigens, and lymphocyte transformation to phytohernagglutinin did not correlate with dissemination of disease. Development of virus-specific complement-fixing antibody (CFA) was delayed in some patients with disseminated disease.Vesicle interferon (V-IF) titers were low early in the disease in patients with localized and disseminated zoster and then rose, usually abruptly, to a peak value and declined as pustulation and crusting occurred. However, titers in patients with localized disease rose at an earlier time. This could be seen in terms of time to development of intermediate values of V-IF or by the day on which the sharpest rise occurred. In 15 carefully studied patients with disseminated disease, the development of the maximum V-IF response was followed within 48 hr by cessation of dissemination. Half of the patients in this group had no CFA detectable until after dissemination had ceased.These findings suggest at least two host factors whose interaction might determine host response to zoster; local interferon production (possibly mediated by sensitized lymphocytes) and humoral antibody, acting to prevent or shorten dissemination of an initially local disease.

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Crude Tissue Culture Antigen for Determination of Varicella-Zoster Complement Fixing Antibody

Abstract: ALTHOUGH the antibody response to varicella-zoster (V-Z) infection may be measured by complement fixation (CF), this

Cited by 54 publications

References 7 publications

Genetic analysis of varicella-zoster virus in the aqueous humor in uveitis with severe hyphema

Genetic analysis of varicella-zoster virus in the aqueous humor in uveitis with severe hyphema

Serological Studies on Two Epidemics of Varicella in a Babies Home

Interferon, antibody, and other host factors in herpes zoster

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