Crude extract of cyanobacterium Radiocystis fernandoi strain R28 induces anemia and oxidative stress in fish erythrocytes

Sakuragui, Marise Margareth; Paulino, Marcelo Gustavo; Souza, Naiara Elisabete da Silva de; Tavares, Driele; Terezan, Ana Paula; Pesenti, Emanuele Cristina; Giani, Alessandra; Fernandes, João B.; Cestari, Marta Margarete; Fernandes, Marisa Narciso

doi:10.1016/j.toxicon.2019.08.002

Cited by 8 publications

(9 citation statements)

References 57 publications

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“…Although many tissues and organs such as blood, gills, heart, kidney, and spleen can be affected by MC causing biochemical, histological, and consequently, functional disruption, 36–39 it is well known that the primary target for MC in the organism is the liver, the main organ to perform a wide range of functions, including energy generation and xenobiotic detoxification 40 …”

Section: Discussionmentioning

confidence: 99%

Liver dysfunction and energy storage mobilization in traíra, Hoplias malabaricus (Teleostei, Erythrinidae) induced by subchronic exposure to toxic cyanobacterial crude extract

Paulino

Rossi

Tavares

et al. 2022

Environmental Toxicology

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Microcystins (MC) are hepatotoxic for organisms. Liver MC accumulation and structural change are intensely studied, but the functional hepatic enzymes and energy metabolism have received little attention. This study investigated the liver and hepatocyte structures and the activity of key hepatic functional enzymes with emphasis on energetic metabolism changes after subchronic fish exposure to cyanobacterial crude extract (CE) containing MC. The Neotropical erythrinid fish, Hoplias malabaricus, were exposed intraperitoneally to CE containing 100 μg MC-LR eq kg À1 for 30 days and, thereafter, the plasma, liver, and white muscle was sampled for analyses. Liver tissue lost cellular structure organization showing round hepatocytes, hyperemia, and biliary duct obstruction. At the ultrastructural level, the mitochondria and the endoplasmic reticulum exhibited disorganization. Direct and total bilirubin increased in plasma. In the liver, the activity of acid phosphatase (ACP) increased, and the aspartate aminotransferase (AST) decreased; AST increased in plasma. Alkaline phosphatase (ALP) and alanine aminotransferase (ALT) were unchanged in the liver, muscle, and plasma. Glycogen stores and the energetic metabolites as glucose, lactate, and pyruvate decrease in the liver; pyruvate decreased in plasma and lactate decreased in muscle. Ammonia levels increased and protein concentration decreased in plasma. CE alters liver morphology by causing hepatocyte intracellular disorder, obstructive cholestasis, and dysfunction in the activity of key liver enzymes. The increasing energy demand implies glucose mobilization and metabolic adjustments maintaining protein preservation and lipid recruitment to supply the needs for detoxification allowing fish survival.

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Section: Discussionmentioning

confidence: 99%

Liver dysfunction and energy storage mobilization in traíra, Hoplias malabaricus (Teleostei, Erythrinidae) induced by subchronic exposure to toxic cyanobacterial crude extract

Paulino

Rossi

Tavares

et al. 2022

Environmental Toxicology

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“…Erythrocyte is one of blood components functioned as an oxygen transporter. Erythrocytes carry O2 to the tissue and release CO2 and protons produced during the metabolism process (Sakuragui et al, 2019)R. fernandoi strain R28, which produces microcystin (MC.…”

Section: Discussionmentioning

confidence: 99%

“…This condition indicates that there are no negative effects after feeding the fish with meniran and garlic powder supplemented feed as fish did not suffer from anemia. Anemia occurs when the hematocrit level is less than 20% (Sakuragui et al, 2019). Meanwhile, hematocrit level greater than 45% is categorized as polycythemia (Grant, 2015)education, and aquaculture evolves.…”

Section: Discussionmentioning

confidence: 99%

Grouper nursery development in sea floating net cage through the application of meniran and garlic powder in feed

Effendi

Pratama

Mulyadin³

et al. 2021

Jurnal Akuakultur Indonesia

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Untuk mendukung usaha budidaya ikan kerapu kini tengah digalakkan sistem pendederan intensif dalam keramba jaring apung (KJA) di laut. Salah satu cara untuk menjaga kondisi ikan tetap sehat dalam sistem tersebut yaitu dengan pemberian fitofarmaka seperti meniran-bawang putih. Tujuan penelitian ini adalah untuk mengevaluasi penambahan tepung meniran-bawang putih melalui pakan terhadap status kesehatan dan kinerja produksi benih ikan kerapu cantang pada pendederan dalam keramba jaring apung di laut. Benih ikan kerapu cantang (panjang 8.27 ± 0.16 cm dan bobot 10.89 ± 0.83 g) dipelihara dalam KJA berupa waring 1x1x1.5 m3 dan diberi pakan dengan penambahan tepung meniran-bawang putih 20 + 25 g/kg pakan selama 7 hari dan 14 hari pertama pemeliharaan, serta tanpa tepung meniran-bawang putih (kontrol) sebagai perlakuan. Ikan dipelihara selama 42 hari dan disampling setiap 2 minggu untuk diambil darah serta diukur bobot dan panjangnya. Penambahan tepung meniran-bawang putih selama 14 hari pada pakan ikan kerapu dapat meningkatkan status kesehatan dan kinerja produksi. Kata kunci: ikan kerapu cantang, kinerja produksi, meniran-bawang putih, status kesehatan.

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“…RBCs are nucleated in birds, reptiles, fish, and amphibians. These blood cells have been used in environmental studies stress [24][25][26][27][28][29][30], and to evaluate the response of xenobiotics in mammals [31][32][33]. However, hardly any studies have been published where environmental pollutants have been quantified in sea turtles [34,35].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Biomarkers in Erythrocytes of Captive Pre-Juvenile Loggerhead Turtles Following Acute Exposure to Methylmercury

et al. 2020

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This study describes the use of erythrocytes (RBCs) of loggerhead turtles as in vitro models for evaluating their toxicity to methylmercury. Blood samples of loggerhead turtles that were born in the Colombian Caribbean were used. The LC50 of RBCs to methylmercury was determined at 96 h using methylmercury concentrations of 0.5–100 mg L−1. Next, the viability of the RBCs and the activity of the enzymes superoxide dismutase (SOD), glutathione S-transferase (GST), and lipid peroxidation by malondialdehyde (MDA) at 6 and 12 h of exposure to acute concentrations of 0, 1, and 5 mg L−1 were evaluated. The LC50 for loggerhead turtle RBCs was 8.32 mg L−1. The cell viability bioassay of RBCs exposed for 12 h only showed 100% cell viability. Increasing in vitro MeHg concentrations caused a corresponding increase in MDA concentration as well as decreases in the activities of SOD and GST. The RBCs represent an excellent model for ecotoxicological studies and SOD, GST, and MDA are biomarkers of environmental pollution and oxidative stress in loggerhead turtles. This was the first study conducted on loggerhead turtle where the response of RBCs to MeHg-induced oxidative stress is evaluated.

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Crude extract of cyanobacterium Radiocystis fernandoi strain R28 induces anemia and oxidative stress in fish erythrocytes

Cited by 8 publications

References 57 publications

Liver dysfunction and energy storage mobilization in traíra, Hoplias malabaricus (Teleostei, Erythrinidae) induced by subchronic exposure to toxic cyanobacterial crude extract

Liver dysfunction and energy storage mobilization in traíra, Hoplias malabaricus (Teleostei, Erythrinidae) induced by subchronic exposure to toxic cyanobacterial crude extract

Grouper nursery development in sea floating net cage through the application of meniran and garlic powder in feed

Oxidative Stress Biomarkers in Erythrocytes of Captive Pre-Juvenile Loggerhead Turtles Following Acute Exposure to Methylmercury

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