Crucial Role of Extracellular Vesicles in Bronchial Asthma

Nagano, Tatsuya; Katsurada, Masahiro; Dokuni, Ryota; Hazama, Daisuke; Kiriu, Tatsunori; Umezawa, Kanoko; Kobayashi, Kazuyuki; Nishimura, Yoshihiro

doi:10.3390/ijms20102589

Cited by 35 publications

(34 citation statements)

References 179 publications

(212 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Copious research has linked an array of miRNAs, likely released in EVs or other carrier molecules, with asthma. EV-miR-145 has been identified as essential to epithelial and smooth muscle cell functions during asthma-induced inflammation 56 , 57 . During asthma, miR-145 is inhibited, preventing eosinophilic inflammation, mucous hypersecretion, Th2 cytokine production, and airway hyperresponsiveness, all of which are physiological responses to bronchial stress such as that induced by asthma 41 , 57 .…”

Section: Physiological Functions Of Evs In Respiratory Diseasesmentioning

confidence: 99%

“… 21 COPD Endothelial cell-derived EVs are upregulated in the lung. 38 , 39 EV-miR-210 controls autophagy functions and differentiation of my fibroblast 42 Pulmonary hypertension 15-LO2-enriched exosomes trigger proliferation of PAECs by activating STAT3 pathway 47 miR-143-enriched exosomes induce migration and angiogenesis of PAECs, 48 Lung fibrosis EV-bound WNT5A promote lung fibroblast proliferation and pathogenesis of lung fibrosis 50 EVs released from mesenchymal stem cells are involved in repairing damaged tissues 51 Asthma EV-miR-145 is essential to epithelial and smooth muscle cell functions during asthma-induced lung inflammation 56 EVs from mast cells, T-cells, dendritic cells, and eosinophils promote asthma-induced lung inflammation 65 , 66 EVs from fibroblasts promote smooth muscle tissue and epithelial cell proliferation in severe asthma 67 …”

Section: Physiological Functions Of Evs In Respiratory Diseasesmentioning

confidence: 99%

See 1 more Smart Citation

Emerging role of extracellular vesicles in the respiratory system

Holtzman

Lee

2020

Exp Mol Med

View full text Add to dashboard Cite

Extracellular vesicles (EVs) present numerous biomedical ways of studying disease and pathology. They function as protective packaging for the delivery of controlled concentrations of miRNAs and effector molecules, including cytokines, chemokines, genetic material, and small signaling molecules. Previous studies of EVs have yielded valuable insights into pathways of intercellular communication that affect a variety of biological processes and disease responses. The roles of EVs, specifically microRNA-containing EVs (EV-miRNAs), in either mitigating or exacerbating pulmonary disease symptoms are numerous and show promise in helping us understand pulmonary disease pathology. Because of their well-documented involvement in pulmonary diseases, EVs show promise both as possible diagnostic biomarkers and as therapeutic agents. This review surveys the physiological functions of EVs in the respiratory system and outlines the pulmonary disease states in which EVs are involved in intercellular crosstalk. This review also discusses the potential clinical applications of EV-miRNAs in pulmonary diseases.

show abstract

Section: Physiological Functions Of Evs In Respiratory Diseasesmentioning

confidence: 99%

Section: Physiological Functions Of Evs In Respiratory Diseasesmentioning

confidence: 99%

Emerging role of extracellular vesicles in the respiratory system

Holtzman

Lee

2020

Exp Mol Med

View full text Add to dashboard Cite

show abstract

“…The greatest differences of EVs subpopulations between AR patients and HS group were identi ed for platelets, endothelium, mastocytes and eosinophil derived EVs. Indeed, during a proin ammatory stimulus an increased amount of EVs released by both platelets and endothelium has been already identi ed as well as for mastocytes derived EVs, speci cally found in greater concentration during an IgE based immune response, a speci c signature of allergic conditions [67][68][69]. The effects of EVs released by mastocytes was also linked to the maturation of the dendritic cells, critical players and sentinels of the immune system in the nasal mucosa during AR [70,71].…”

Section: Discussionmentioning

confidence: 98%

Characterization and Analysis of The Nasal Microbiota and Plasmatic Extracellular Vesicles in Allergic Rhinitis: A Case-Control Study

Mariani¹,

Iodice²,

Cantone³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Increasing evidence suggests a possible link between the bacterial nasal microbiota (bNM) to allergic diseases such as allergic Rhinitis (AR), as it might modulate the allergic response by acting on the onset and progression of allergic inflammation. In this case-control study, we aimed to compare the bNM between 25 AR patients and 25 matched healthy subjects (HS) as well as to evaluate possible modifications within the host-microbiota cross-talk, investigating the release pattern of both bacterial- and host-Extracellular Vesicles (EVs).Results: bNM analysis showed that Actinobacteria (AR: 2.5%-83.5%; HS: 18.6%-92.7%), Firmicutes (AR: 6.1%-63.5%; HS: 6.4%-72.2%) and Proteobacteria (AR: 0.6%-89.6%; HS 0.7%-38%) were the most abundant phyla in the study population. Diversity reduction in AR patient bNM was pointed out compared to the HS group bNM (Observed OTUs: p =0.018; PD whole tree: p =0.014). Moreover, the distribution of the weighted normalized intra-group UniFrac distances analysis showed a less uniform bacterial community within AR patients rather than HS group (nonparametric test p =0.01). Since EVs may have a central role in the cross-talk between bNM and the host, we evaluated their size and concentration in the plasma of the study subjects. AR patients were characterized by a higher concentration of EVs ranging between 130-231 nm, 257-287 nm and 323-348 nm (p <0.05). We further characterized plasmatic EVs investigating both bacterial-EVs and host-EVs subpopulations and observed that all EV subtypes mean concentrations were higher in AR patients compared to those of HS group, except for EpCAM+ EVs.Conclusion: Our results suggest that AR patients are characterized by a less diverse and wide intra-group variable bNM compared to that of HS, as well as an altered host-microbiota EV communication network. Further studies are needed to disentangle the relationship between the host and the nasal bacterial community during pathological conditions and health.

show abstract

“…ey exist in biological fluids and play pivotal roles in multiple physiological and pathological processes [9]. Recently, the crucial role of exosomes in bronchial asthma has been discovered [10]. Bronchoalveolar lavage fluid (BALF) exosomes are involved in the cytokine and leukotriene production in allergic asthma [11].…”

Section: Introductionmentioning

confidence: 99%

Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede

Tang

Fang

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Asthma is a common respiratory disease with inflammation in the lungs. Exosomes and microRNAs (miRNAs) play crucial role in inflammation, whereas the role of exosomal miRNA in asthma remains unknown. Here, we aimed to identify the key exosomal miRNAs and their underlying mechanisms involved in scorpio and centipede (SC) treatment in asthma. Eighteen mice were randomly divided into three groups: control group, asthma group, and SC treatment group. Effect of SC was assessed by hematoxylin-eosin staining and real-time PCR. Exosomes from asthma and SC treatment groups were analyzed by small RNA-seq. Results revealed SC significantly alleviated the pathogenesis of asthma and suppressed the release of inflammatory cytokines. A total of 328 exosomal miRNAs were differentially expressed between the exosomes from asthma and SC-treated mice, including 118 up- and 210 downregulated in SC-treated mice. The altered exosomal miRNAs were primarily involved in the function of transcription, apoptotic process, and cell adhesion; and pathway of calcium, Wnt, and MAPK signaling. Real-time PCR verified exosomal miR-147 was downregulated, while miR-98-5p and miR-10a-5p were upregulated in SC-treated mice compared to asthma mice. Moreover, the target genes of miR-147-3p, miR-98-5p, and miR-10a-5p were mainly enriched in Wnt and MAPK inflammatory signaling. miR-10a-5p promoted the proliferation of mouse lung epithelial cells and downregulated the expression of Nfat5 and Map2k6. These data suggest SC-induced exosomal miRNAs might mediate the inflammatory signaling and might be involved in the SC treatment in asthma. The exosomal miRNAs might be promising candidates for the treatment of asthma.

show abstract

Crucial Role of Extracellular Vesicles in Bronchial Asthma

Cited by 35 publications

References 179 publications

Emerging role of extracellular vesicles in the respiratory system

Emerging role of extracellular vesicles in the respiratory system

Characterization and Analysis of The Nasal Microbiota and Plasmatic Extracellular Vesicles in Allergic Rhinitis: A Case-Control Study

Small RNA Sequencing Reveals Exosomal miRNAs Involved in the Treatment of Asthma by Scorpio and Centipede

Contact Info

Product

Resources

About