Crossprotective immunity between the Florida and a Zimbabwe stock ofAnaplasma marginale

Tebele, N; Palmer, Guy H.

doi:10.1007/bf02357100

Cited by 19 publications

(9 citation statements)

References 13 publications

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“…Organisms. A. marginale isolated in 1988 from a holstein steer with acute clinical anaplasmosis was designated the Norton Zimbabwe strain after the geographic area of isolation (29). The strain was maintained as a cryopreserved stabilate (10).…”

Section: Methodsmentioning

confidence: 99%

“…However, when challenged with a more virulent field strain, animals immunized by premunition are susceptible to infection and severe disease (19). Such incomplete protection was observed in cattle recovered from infection with the Florida strain and challenged with the Norton Zimbabwe strain and indicated the presence of both conserved and strain-specific protection-inducing antigens (29).…”

mentioning

confidence: 98%

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Induction of protective immunity by using Anaplasma marginale initial body membranes

Tebele¹,

McGuire²,

Palmer³

1991

Infect Immun

122

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

Induction of protective immunity by using Anaplasma marginale initial body membranes

Tebele¹,

McGuire²,

Palmer³

1991

Infect Immun

122

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“…For vaccine production, splenectomized calves maintained under quarantine conditions are experimentally inoculated with defined strains and serve as a source of infective blood. Vaccinated cattle develop persistent infections, which induce lifelong protective immunity in cattle, and revaccination is usually not required (1,139,160,175). Vaccination strategies using live organisms include (i) infection and treatment, (ii) live vaccines containing attenuated strains of A. marginale, and (iii) live vaccines containing the less pathogenic A. centrale.…”

Section: Live Vaccinesmentioning

confidence: 99%

Antigens and Alternatives for Control ofAnaplasma marginaleInfection in Cattle

et al. 2003

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Anaplasmosis, a tick-borne cattle disease caused by the rickettsia Anaplasma marginale, is endemic in tropical and subtropical areas of the world. The disease causes considerable economic loss to both the dairy and beef industries worldwide. Analyses of 16S rRNA, groESL, and surface proteins have resulted in the recent reclassification of the order Rickettsiales. The genus Anaplasma, of which A. marginale is the type species, now also includes A. bovis, A. platys, and A. phagocytophilum, which were previously known as Ehrlichia bovis, E. platys, and the E. phagocytophila group (which causes human granulocytic ehrlichiosis), respectively. Live and killed vaccines have been used for control of anaplasmosis, and both types of vaccines have advantages and disadvantages. These vaccines have been effective in preventing clinical anaplasmosis in cattle but have not blocked A. marginale infection. Thus, persistently infected cattle serve as a reservoir of infective blood for both mechanical transmission and infection of ticks. Advances in biochemical, immunologic, and molecular technologies during the last decade have been applied to research of A. marginale and related organisms. The recent development of a cell culture system for A. marginale provides a potential source of antigen for the development of improved killed and live vaccines, and the availability of cell culture-derived antigen would eliminate the use of cattle in vaccine production. Increased knowledge of A. marginale antigen repertoires and an improved understanding of bovine cellular and humoral immune responses to A. marginale, combined with the new technologies, should contribute to the development of more effective vaccines for control and prevention of anaplasmosis

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“…Os altos índices de identidade do gene msp5 da amostra PR1 de A. marginale com a amostra Flórida e Saint Maries (98%), Havana e Brasil-PE (97%) e A. centrale (91%) confirmam a conservação da MSP5 nos diferentes isolados de diferentes regiões (TEBELE et al, 1991;VISSER et al, 1992;NDUNG'U et al, 1995).…”

Section: Discussionunclassified

Clonagem, expressão, caracterizaÇão molecular da proteína de superfície MSP5 da amostra PR1 de Anaplasma marginale e sua aplicaÇão em um teste de ELISA por competiÇão

Marana¹,

Kano²,

Vicentini³

et al. 2009

RBPV

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ResumoNo presente trabalho, um teste imunoenzimático por competição (cELISA) foi padronizado com a proteína recombinante de superfície rMSP5, clonada a partir do gene msp5 do isolado PR1 de A. marginale. O sequenciamento mostrou que o gene que codifica a rMSP5/PR1 tem 98% de identidade com os isolados Flórida e Santa Maria, 97% com isolados de Pernambuco, Brasil e Havana, Cuba e 91% com A. centrale. O teste de cELISA-PR1 foi comparado com os testes de IFI e cELISA-USA. Para a padronização e validação do cELISA-PR1, foram utilizados 380 soros bovinos comprovadamente positivos ou negativos pelo cELISA-USA. Desse total, 245 soros positivos eram de animais de área endêmica e 135 soros eram negativos, de área livre de anaplasmose. Na padronização, foram utilizados 283 soros de bovinos, dos quais 135 eram negativos e 148 positivos. Os testes de cELISA-PR1 e IFI apresentaram especificidade de 100 e 99,3%, sensibilidade de 100 e 98%, com coeficiente kappa de 0,993 e 0,978, respectivamente. Na validação do teste, foram utilizados 245 soros de bovinos de áreas endêmicas para anaplasmose, testados pelo cELISA-PR1 e IFI e apresentaram especificidade de 96,7 e 69,1%, sensibilidade de 98,9 e 96,3% e coeficiente kappa de 0,956 e 0,699, respectivamente. Esses resultados permitem afirmar que o teste de cELISA-PR1 apresentou performance equivalente ao cELISA-USA, podendo também ser utilizado em estudos epidemiológicos, programas de controle e movimentação internacional de animais, enquanto a IFI, com os resultados menos precisos apresentados, não deve ser utilizada em situações que requerem maior rigor no diagnóstico.Palavras-chave: Anaplasma marginale, ELISA, IFI, proteínas, bovinos. AbstractA competitive enzyme-linked immunosorbent test using the PR1 recombinant major surface protein 5 (rMSP5-PR1-ELISA)of Anaplasma marginale was standardized and validated using sera from anaplasmosis free and endemic regions. The sequencing of the msp5 gene of PR1 isolate showed 98% of identity with the Florida and Saint Maries isolates, 97% with Brazil (Pernambuco) and Havana isolates; and 91% with A. centrale. The cELISA-PR1 test was compared to IFI and cELISA-USA. For the standardization and validation of the cELISA-PR1, 380 bovine sera were used, whereas 245 truly positives and 135 truly negatives sera tested by the cELISA-USA. In the standardization of the cELISA-PR1 135 negative and 148 positive bovine sera were used. The cELISA-PR1 and IFI tests showed 100 and 99.3% specificity, 100 and 98%, sensibility, and a kappa coefficient of 0.993 and 0.978, respectively. For test validation, 245 bovine sera from an anaplasmosis endemic area were analyzed by the cELISA-PR1 and IFI, which showed 96.7 and 69.1% specificity, 98.9 e 96.3% sensibility and kappa coefficient of 0.956 and 0.699, respectively. These results indicate that the cELISA-PR1, likewise the cELISA-USA, could sensitively and specifically detect cattle naturally infected with A. marginale and would be recommended for epidemiological studies, eradications program, and regulation of in...

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Crossprotective immunity between the Florida and a Zimbabwe stock ofAnaplasma marginale

Cited by 19 publications

References 13 publications

Induction of protective immunity by using Anaplasma marginale initial body membranes

Induction of protective immunity by using Anaplasma marginale initial body membranes

Antigens and Alternatives for Control ofAnaplasma marginaleInfection in Cattle

Clonagem, expressão, caracterizaÇão molecular da proteína de superfície MSP5 da amostra PR1 de Anaplasma marginale e sua aplicaÇão em um teste de ELISA por competiÇão

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