Induction of protective immunity by using Anaplasma marginale initial body membranes

Abstract

Anaplasma marginale initial bodies of the Norton Zimbabwe strain were disrupted and separated into two membrane fractions banding at 1.15 and 1.22 g/cm3 by sucrose density centrifugation. The membrane fractions

Immunization of Bovines Using a DNA Vaccine (pcDNA3.1/MSP1b) Prepared from the Jaboticabal Strain of Anaplasma marginale

“…Furthermore, serum antibody titers in calves detected by serological assays were not enough to achieve a total protection against anaplasmosis, although high titers of antibodies were reported as responsible for protection level. 7 In our experiment, it was not significant at first sight since antibodies developed by immunized calves were directed toward MSP1b and not to other MSPs from A. marginale. Moreover, the lack of protection observed in immunized calves in comparison with nonvaccinated calves could be explained by the polymorphism encoded in the variant MSP1b proteins since just one protein was cloned.…”

“…6 Immunization of cattle using killed whole organisms or purified outer membranes has been shown to induce partial protection against high-level rickettsemia and severe disease. 7 The use of inactivated A. marginale immunization to protect cattle has been reported and attempts to make improved vaccine have been made by purifying and using specific surface membrane proteins. 3 Six major surface proteins (MSPs) from A. marginale have been well characterized, which include MSP1a, MSP1b, MSP2, MSP3, MSP4, and MSP5.…”

Genome-wide screening and identification of antigens for rickettsial vaccine development: 1

“…Immunization with A. marginale outer membranes, isolated from intact bacteria using density gradient centrifugation, induces protection as measured by significant reduction in bacteremia following challenge with complete protection against infection in 20-30% of vaccinates (Tebele et al, 1991;Brown et al, 1998;Noh et al, 2008). Bioinformatic identification of outer membrane proteins (Omp) in the A. marginale genome using PSORT, PSORTB, and homology to experimentally identified surface proteins predicted 62 Omps within the 949 proteins predicted to represent the complete proteome (Brayton et al, 2005).…”

“…The outer membrane immunogen used in the initial screen included all proteins in the isolated fraction (Tebele et al, 1991;Lopez et al, 2005). To narrow the candidate list to only those proteins with surface-exposed domains, a macromolecular surface complex was isolated by cross-linking proteins on intact bacteria using 3,3′-dithiobis[sulfosuccinimidylpropionate], reactive with primary amines with a spacer arm of 1.2 nm, followed by electrophoretic separation (Noh et al, 2008).…”

Cloning and expression of the recombinant polypeptide based on the Anaplasma marginale MSP1b protein in Escherichia coli

“…Journal "Veterinaria i kormlenie" Ââåäåíèå Anaplasma marginale (ïîðÿäîê Riñkettsiales, ñåìåéñòâî Anaplasmatacåa), îáëèãàòíûé âíóòðèýðèòðîöèòàðíûé ïàòîãåí, ÿâëÿåòñÿ âîçáóäèòåëåì àíàïëàçìîçà êðóïíîãî ðîãàòîãî ñêîòà, ðàñïðîñòðàí¸ííîãî ïî âñåìó ìèðó è íàíîñÿùåãî ñåðüåçíûé ýêîíîìè÷åñêèé óùåðá [1,11]. Áûëî ïîêàçàíî, ÷òî èììóíèçàöèÿ aeèâîòíûõ î÷èùåííûìè íàðóaeíûìè ìåìáðàíàìè âûçûâàåò çàùèòó îò îñòðîé èíôåêöèè A. marginale [24]. Ðÿä áåëêîâ íàðóaeíîé ìåìáðàíû (MSP -major surface proteins), òàêèå êàê MSP1α, MSP1β, MSP2, MSP3, MSP4, MSP5 è VirB9 è VirB10, áûëè îõàðàêòåðèçîâàíû êàê ïîòåíöèàëüíûå êàíäèäàòû íà ðîëü àíòèãåíîâ äëÿ ïðîèçâîäñòâà âàêöèí ïðîòèâ A. marginale è äëÿ ñåðîëîãè÷åñêîé äèàãíîñòèêè àíàïëàçìîçà êðóïíîãî ðîãàòîãî ñêîòà [19,12,25].…”