Crossed Immunoelectrophoresis and Crossed-line Immunoelectrophoresis of Salmonella dublin Antigens

Brown, Grafton Tyler; Jones, Phillip W.

doi:10.1099/00221287-116-2-315

Cited by 8 publications

(6 citation statements)

References 8 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The precipitin pattern of 82 anodic-migrating antigens was very complex, similar to the results obtained with other gram-negative bacterial species by the same methods (7,14,21,23,26,41,43,44), and more closely approximates a demonstration of the total antigenic profile suggested by Joly and Kenney (28).…”

Section: Resultssupporting

confidence: 83%

Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens

Collins¹,

Cho²,

Høiby³

et al. 1983

Infect Immun

View full text Add to dashboard Cite

By crossed immunoelectrophoresis, 85 different antigens were demonstrated in sonicated preparations of Legionella pneumophila serogroup 1 (Lp1). The precipitin patterns of 82 anodic-migrating antigens were numbered and were designated the Lp1 reference system. Eleven antigens were stable to boiling, and seven of these were shown to be surface antigens. One heat-stable surface antigen (antigen no. 61) was highly reactive with limulus amoebocyte lysates and formed a precipitin resembling lipopolysaccharide. Serum from an isolation confirmed case of Lp1 infection and serogroup-specific rabbit antiserum reacted specifically with antigen no. 61, which was designated the serogroup-specific antigen. Normal human and rabbit sera commonly had antibodies to antigen no. 66 of the Lp1 reference system. This antigen is antigenically related to the "common antigen" of Pseudomonas aeruginosa.

show abstract

Section: Resultssupporting

confidence: 83%

Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens

Collins¹,

Cho²,

Høiby³

et al. 1983

Infect Immun

View full text Add to dashboard Cite

show abstract

“…A complex antigenic composition of S. epidermidis was found. This, as well as the findings of a high degree of cross-reactivity between strains of the same species, is in agreement with results obtained with quantitative immunoelectrophoretic methods employed in the study of other bacterial species (6,9,17).…”

Section: Discussionsupporting

confidence: 91%

CROSS‐REACTIONS BETWEEN STAPHYLOCOCCUS EPIDERMIDIS AND 23 OTHER BACTERIAL SPECIES

Espersen

Hertz

Høiby

1981

Acta Pathologica Microbiologica Scandinavica Section B Microbio

View full text Add to dashboard Cite

Espersen, F., Hertz, J. B. & Hsiby, N. Cross-reactions between Staphylococcus epidermidis and 23 other bacterial species. Acta path. microbiol. scand. Sect. B, 89: 253-260, 1981. By quantitative immunoelectrophoretic methods, 43 antigens were found in a mixture of sonicated preparations of four Staphylococcus epidermidis strains, using corresponding rabbit antiserum. Two of the antigens were identified as cell wall teichoic acid and a peptidoglycan antigen, respectively. Using this antigen/antibody reference system, cross-reactions between S. epidermidis antigens and antigens from other bacterial species were investigated. Fourteen of the S. epidermidis antigens cross-reacted with antigens from all S. aureus strains investigated. Only few cross-reactions were found between S. epidermidis and bacteria not belonging to the Micrococcaceae. The antigenic relatedness, expressed as a matching coefficient, seems promising for taxonomic work.The antigenic structure of S. epidermidis has been investigated by different methods ( I , 8, 12, 14, 32).Most investigators have focused on teichoic acid and peptidoglycan antigens found in the cell wall; other antigens have received less attention. Characterization of the antigens of this organism would seem to be important because of its increasing significance in human infections.The purpose of the present study was to investigate the antigenic components of S. epidermidis by quantitative immunoelectrophoretic techniques and to study cross-reactivity of S. epidermidis with other bacteria, especially other Micrococcaceae. MATERIALS AND METHODSStrains. Four Staphylococcus epidermidis strains, representing the four biotypes of Baird-Parker (41, were chosen as reference strains. AU were isolated from blood cultures of patients with bacteraemia. The strains were provided by Dr. K. Rosendal, Statens Seruminstitut, Copenhagen, who also performed the biotyping. The main biochemical characteristics of these strains are given in Table I .The origin of many of the other bacteria shown in Table 3 have been described previously (18, 31). The collection strains of Micrococcaceae, NCTC (National Collection of Type Cultures, London) 4163,8532, 761 2 and 7292; CCM (Czechoslovak Collection of Microorganisms, Brno) 901 and 385; ACBP (Dr. A . C. Baird-Parker, England) 3, 5 and 9, were provided by Dr. N . Mortensen, Institute for Medical Microbiology, University of Copenhagen. Cowan 1-111, Wood 46 and S. aureus strains belonging to the 4 phage groups (Collection numbers E 237 I , E 2476, E 1346 and E 1430) were provided by Dr. K. Rosendal, Statens Seruminstitut. Peptococcus micron and Clostridium perfringens were provided by Dr. M. Tvede, Statens Seruminstitut.Antigens. Antigens for immunoelectrophoretic experiments were obtained by sonication (18). Reference antigen (Ref. Ag.) was a mixture of equal volumes from each reference strain, the colloid concentration was 6.9 g/l (refractometry, using human IgG as standard).Antigens for investigation of heat-stability were prepared by autoclaving Ref . A...

show abstract

“…66 was similar to antigens no. 4,9,10,11, and 19 of the Haemophilus influenzae (54), Salmonella typhi (19), P. aeruginosa (31), Bordetella pertussis (28), and N. meningitidis (29) XIE systems, respectively, all of which have been shown to cross-react extensively with many gram-negative bacteria. Antigen no.…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of L. pneumophila antigens by crossed immunoelectrophoresis (XIE) in our laboratory was previously described, and a reference system with 82 antigens was established (11). XIE techniques are well-suited for the study of cross-reactions between bacterial species and have been widely used for this purpose (10,18,20,25,30,32,34,54,63). Joly and Kenny (38) used the XIE technique to study cross-reactions between L. pneumophila SGs 1, 2, 3, and 4 and Tatlockia (Legionella) micdadei.…”

mentioning

confidence: 99%

Cross-reactions between Legionella pneumophila (serogroup 1) and twenty-eight other bacterial species, including other members of the family Legionellaceae

Collins¹,

Espersen²,

Høiby³

et al. 1983

Infect Immun

View full text Add to dashboard Cite

Cross-reactions between Legionella pneumophila serogroup 1 and 28 other bacterial species were studied by various quantitative immunoelectrophoretic techniques. A sonicated L. pneumophila antigen and purified homologous rabbit antibody were used as a reference system. Few antigens (0 to 6) cross-reacted with non-Legionellaceae, but two were found in nearly all gram-negative bacteria tested (antigens no. 1 and 66). Antigen no. 66 of the L. pneumophila reference system was shown to be antigenically similar to the "common antigen" of Pseudomonas aeruginosa reported in many gram-negative bacteria. Greater than 85% of the antigens from L. pneumophila serogroup 1 cross-reacted with the other six serogroups of L. pneumophila. By contrast, Fluoribacter (Legionella) bozemanae, F. (L.) dumoffii, F. (L.) gormanii, and Tatlockia (Legionella) micdadei cross-reacted with only 45, 53, 39, and 43% of the reference system antigens, respectively. The antigenic relatedness of members of the Legionellaceae, expressed as a matching coefficient, is discussed in terms of its taxonomic significance. Serogroup-, genus-, and family-specific antigens are identified in the L. pneumophila reference system. Several serological studies have described cross-reactions between serogroups (SGs) of Legionella pneumophila and between L. pneumophila and other bacteria, leading to speculations concerning SG-specific antigens, Legionella-common antigens and antigens common to Legionella spp. and other bacterial species (13,39,49,(65)(66)(67). SG-specific antigens have been demonstrated by five different groups of investigators, all using precipitation reactions, but each using different antigen extraction methods (11,16,38,56,58). These studies indicated that the SG-specific antigen of L. pneumophila was a high-molecular-weight, heat-stable surface antigen with the physiochemical properties of lipopolysaccharide.Antigens common to several members of the Legionellaceae also have been demonstrated (15,38,68), but Legionella-common antigen or antigens, which are present in all Legionella spp. and not cross-reactive with other bacteria, have not been reported. As the number of antigenic types of Legionellaceae continues to grow, the need for identification and isolation of t Present address: Department of Microbiology, Colorado State University, Fort Collins, CO 80523. 1441 on August 1, 2020 by guest http://iai.asm.org/ Downloaded from 33 21 21 21 21 21reference system (11) which were SG-specific, those which were common only to members of the Legionellaceae, and those which were crossreactive with other bacteria. MATERIALS AND METHODSLpl reference system. The Lpl XIE reference system has been described previously, in a study in which 82 anodic-migrating immunoprecipitates were also INFECT. IMMUN.on August 1, 2020 by guest http://iai.asm.org/ Downloaded from

show abstract

Crossed Immunoelectrophoresis and Crossed-line Immunoelectrophoresis of Salmonella dublin Antigens

Cited by 8 publications

References 8 publications

Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens

Crossed immunoelectrophoretic analysis of Legionella pneumophila serogroup 1 antigens

CROSS‐REACTIONS BETWEEN STAPHYLOCOCCUS EPIDERMIDIS AND 23 OTHER BACTERIAL SPECIES

Cross-reactions between Legionella pneumophila (serogroup 1) and twenty-eight other bacterial species, including other members of the family Legionellaceae

Contact Info

Product

Resources

About