Cross-validated stable-isotope dilution GC–MS and LC–MS/MS assays for monoacylglycerol lipase (MAGL) activity by measuring arachidonic acid released from the endocannabinoid 2-arachidonoyl glycerol

Kayaçelebi, Arslan Arinç; Schauerte, Celina; Kling, Katharina Maria; Herbers, Jan; Beckmann, Bibiana; Engeli, Stefan; Jordan, Jens; Zoerner, Alexander A.; Tsikas, Dimitrios

doi:10.1016/j.jchromb.2016.08.004

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…To measure MAGL activity, assays can be performed using deuterium-labeled 2AG (d8-2AG) such as the MAGL substrate and measure deuterium-labeled AA (d8-AA) as the MAGL product in biological samples [129]. Furthermore, the enzyme activity can also be measured using fluorometric methods, as already discussed.…”

Section: Alterations In Protein Activitymentioning

confidence: 99%

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Navarro

Gasparyan

Navarrete

et al. 2022

IJMS

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The therapeutic benefits of the current medications for patients with psychiatric disorders contrast with a great variety of adverse effects. The endocannabinoid system (ECS) components have gained high interest as potential new targets for treating psychiatry diseases because of their neuromodulator role, which is essential to understanding the regulation of many brain functions. This article reviewed the molecular alterations in ECS occurring in different psychiatric conditions. The methods used to identify alterations in the ECS were also described. We used a translational approach. The animal models reproducing some behavioral and/or neurochemical aspects of psychiatric disorders and the molecular alterations in clinical studies in post-mortem brain tissue or peripheral tissues were analyzed. This article reviewed the most relevant ECS changes in prevalent psychiatric diseases such as mood disorders, schizophrenia, autism, attentional deficit, eating disorders (ED), and addiction. The review concludes that clinical research studies are urgently needed for two different purposes: (1) To identify alterations of the ECS components potentially useful as new biomarkers relating to a specific disease or condition, and (2) to design new therapeutic targets based on the specific alterations found to improve the pharmacological treatment in psychiatry.

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Section: Alterations In Protein Activitymentioning

confidence: 99%

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Navarro

Gasparyan

Navarrete

et al. 2022

IJMS

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“…For instance, the perfluoro group presents high electron affinity, being suitable for NCI. In this manner, an esterification process of AA and PGE 2 was accomplished using pentafluorobenzyl bromide (PFB-Br) as derivatization agent, allowing the controlled fragmentation of both metabolites and accordingly, resulting in a highly sensitive quantitative method [18]. Additionally, PCI has been used for the determination of AEA by GC-MS using TMS as derivatization agent.…”

Section: Endocannabinoidsmentioning

confidence: 99%

Exploring the Mysteries of Cannabis through Gas Chromatography

García-Valverde¹,

Medina²,

Codesido³

et al. 2022

Recent Advances in Gas Chromatography

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In the last decades, cannabinoids, the active constituents of Cannabis sativa L., have been attracting a strong interest, regarding the health effects associated with the use of Cannabis and Cannabis-derived products. The progressive legalization of this species in several countries has prompted an increasing concern about the characterization and quantification of cannabinoids in diverse chemotypes of the plant, as well as the obtained final products. Therewith, Process and Product Quality Assurance (PPQA) becomes a mandatory practise to verify the Good Manufacturing Practices (GMP). Gas chromatography is one of the most used techniques in this sense due to its high attainable resolution. However, sample complexity and the thermal lability of cannabinoids hinder the analysis. In this chapter, a fully description of the recent advances in the Cannabis sativa L. analysis by gas chromatography will be presented, including different approaches that have come up to solve the obstacles encountered.

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Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

Amir Hamzah,

Turner,

Nichols

et al. 2024

Mass Spectrometry Reviews

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Liquid chromatography paired with tandem mass spectrometry (LC‐MS/MS) is the gold standard in measurement of endocannabinoid concentrations in biomatrices. We conducted a systematic review of literature to identify advances in targeted LC‐MS/MS methods in the period 2017–2024. We found that LC‐MS/MS methods for endocannabinoid quantification are relatively consistent both across time and across biomatrices. Recent advances have primarily been in three areas: (1) sample preparation techniques, specific to the chosen biomatrix; (2) the range of biomatrices tested, recently favoring blood matrices; and (3) the breadth of endocannabinoid and endocannabinoid‐like analytes incorporated into assays. This review provides a summary of the recent literature and a guide for researchers looking to establish the best methods for quantifying endocannabinoids in a range of biomatrices.

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Cross-validated stable-isotope dilution GC–MS and LC–MS/MS assays for monoacylglycerol lipase (MAGL) activity by measuring arachidonic acid released from the endocannabinoid 2-arachidonoyl glycerol

Cited by 6 publications

References 16 publications

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Exploring the Mysteries of Cannabis through Gas Chromatography

Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

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