Cross‐talk between ovarian cancer cells and macrophages through periostin promotes macrophage recruitment

Meng, Tong; Liu, Bingji; Bu, Xiaocui; Zhao, Peng

doi:10.1111/cas.13567

Cited by 34 publications

(29 citation statements)

References 35 publications

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“…Nowadays, the tumor infiltrating inflammatory cells are mainly considered as tumor associated macrophages (TAMs), which play an important role in tumorigenesis, cancer invasion and metastasis [11, 12]. Recent studies have shown that the interaction of TAM and OCSLCs is involved in the occurrence, recurrence and multidrug resistance of ovarian cancers [13, 14]. Our previous study showed that THP-1 macrophages co-cultured with SKOV3-derived OCSLCs contain the characteristics of TAMs [15].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis

Ning

Feng

Cao

et al. 2019

J Exp Clin Cancer Res

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BackgroundRecent studies showed that macrophages co-cultured with ovarian cancer stem-like cells (OCSLCs) induced SKOV3 cell stemness via IL-8/STAT3 signaling. Genistein (GEN) demonstrates chemopreventive activity in inflammation-associated cancers. The present study aimed to examine whether and if GEN inhibits the stemness of SKOV3 and OVCA-3R cells induced by co-culture of THP-1 macrophages and SKOV3-derived OCSLCs.MethodsThe co-culture was treated with or without different concentrations (10, 20, and 40 μmol/L) of GEN for 24 h. Depletion or addition of IL-8 in Co-CM and knockdown or overexpression of STAT3 in THP-1 macrophages was performed to demonstrate the possible associated mechanisms. The combined effects of GEN and STAT3 knockdown were examined with the nude mouse modle by co-injection of SKOV3-derived OCSLCs with THP-1 macrophages.ResultsOur results showed that GEN down-regulated CD163 and p-STAT3 expression of THP-1 macrophage, decreased the levels of IL-10, increased the levels of IL-12 and nitric oxide (NO) in the conditioned medium, and reduced the clonogenic and sphere-forming capacities and the expression of CD133 and CD44 in SKOV3 cells induced by co-culture of THP-1 macrophages and OCSLCs in a dose-dependent manner. Moreover, depletion or addition of IL-8 enhanced or attenuated the effect of GEN. Additionally, knockdown or overepression of STAT3 in THP-1 macrophages potentiated or attenuated the inhibitory effects of GEN. Importantly, STAT3 overexpression retrieved the effects of IL-8 combined with GEN depletion on M2 polarization of THP-1 macrophages and stemness of SKOV3 cells induced by co-culture. The combination of GEN and STAT3 knockdown cooperatively inhibited the growth of tumors co-inoculated with OCSLCs/THP-1 macrophages in nude mice in vivo through blocking IL-8/STAT3 signaling.ConclusionsIn summary, our findings suggested that GEN can inhibit the increased M2 polarization of macrophages and stemness of ovarian cancer cells by co-culture of macrophages with OCSLCs through disrupting IL-8/STAT3 signaling axis. This assisted GEN to be as a potential chemotherapeutic agent in human ovarian cancer.Electronic supplementary materialThe online version of this article (10.1186/s13046-018-1010-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis

Ning

Feng

Cao

et al. 2019

J Exp Clin Cancer Res

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“…Ovarian cancer cell lines and tumor biopsy specimens express TNF, CCL22, and CXCL12, which induce macrophage polarization into the M2 phenotype in the TME (67). Periostin from ovarian cancer cells was shown to be a key factor in M2 macrophage recruitment (68). SEMA4D is a member of the transmembrane or secretory signaling protein IV subfamily.…”

Section: Roles Of Macrophage Polarization In the Development And Progmentioning

confidence: 99%

Macrophage Polarization in the Development and Progression of Ovarian Cancers: An Overview

et al. 2019

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Ovarian cancer is the most lethal gynecological malignancy worldwide. Most patients are diagnosed at late stages because of atypical symptoms and the lack of effective early diagnostic measures. The mechanisms underlying the oncogenesis and development of ovarian cancer are not clear. Macrophages, immune cells derived from the innate immune system, have two states of polarization (M1 and M2) that develop in response to different stimuli. The polarization and differentiation of macrophages into the cancer-inhibiting M1 and cancer-promoting M2 types represent the two states of macrophages in the tumor microenvironment. The interaction of polarized macrophages with cancer cells plays a crucial role in a variety of cancers. However, the effects of macrophage M1/M2 polarization on ovarian cancer have not yet been systematically and fully discussed. In this review, we discuss not only the occurrence, development and influences of macrophage polarization but also the association between macrophage polarization and ovarian cancer. The polarization of macrophages into the M1 and M2 phenotypes plays a pivotal role in ovarian cancer initiation, progression, and metastasis, and provides targets for macrophage-centered treatment in the cancer microenvironment for ovarian cancer therapy. We also addressed the regulation of macrophage polarization in ovarian cancer via noncoding RNAs, exosomes, and epigenetics.

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“…One of the major constituents of the tumor microenvironment is TAMs, which is also known as tumor-infiltrating macrophages 45–47. Studies showed that TAMs promote tumor initiation, development, and distant metastasis by enhancing the tumor angiogenesis, invasion, and migration ability 28. Macrophage is one of the major components that infiltrates most tumors.…”

Section: Discussionmentioning

confidence: 99%

“…Diffuse GC usually originates from the gastric mucosa and mostly occurs in younger patients because of a chronic active inflammation 35. Periostin has immunological roles in various malignancies and diseases 28,30,31. Studies found that periostin recruit macrophages through integrin alpha-v-beta-3 26.…”

Section: Discussionmentioning

confidence: 99%

Low periostin expression predicts poor survival in intestinal type gastric cancer patients

Zhang¹,

Zheng²,

Chandoo³

et al. 2018

CMAR

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Background and aimPeriostin is a protein from the Fascilin family. It is commonly present in normal tissues and is responsible for cell adhesion. Evidence has emerged showing that changes in periostin expression play an important role in tumor initiation, development, and progression. This study aims to investigate the effect of periostin in gastric cancer (GC) patients who underwent gastrectomy. Seven hundred and forty-seven GC patients who underwent gastrectomy between December 2006 and July 2011 were included in this study.MethodsSeven hundred and forty-seven cancer tissues and 70 paired adjacent normal tissues were collected. Periostin expression was evaluated by immunohistochemistry. The Gene Expression Omnibus database was used to study the association between the mRNA level and patient’s overall survival. The tumor microenvironment was also studied.ResultsPeriostin expression in stroma was downregulated in tumor tissues but it was upregulated in the epithelial cells. After dividing the tissues according to the Lauren Classification, we found that periostin expression in stroma and epithelial cells was higher in intestinal type than in diffuse type (P<0.001 and P=0.010, respectively). Periostin was an independent predictor of lymph node (LN) metastasis in GC patients. The study of CD163(+) tumor-associated macrophages (TAMs) revealed that in diffuse type GC, periostin expression was associated with CD163(+) TAMs.ConclusionWe found that the periostin expression can predict LN metastasis in patients undergoing curative gastrectomy. Intestinal type GC patients with high periostin level had both a favorable survival and lesser LN metastasis.

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Cross‐talk between ovarian cancer cells and macrophages through periostin promotes macrophage recruitment

Cited by 34 publications

References 35 publications

RETRACTED ARTICLE: Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis

RETRACTED ARTICLE: Genistein inhibits stemness of SKOV3 cells induced by macrophages co-cultured with ovarian cancer stem-like cells through IL-8/STAT3 axis

Macrophage Polarization in the Development and Progression of Ovarian Cancers: An Overview

Low periostin expression predicts poor survival in intestinal type gastric cancer patients

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