Low periostin expression predicts poor survival in intestinal type gastric cancer patients

Abstract

Background and aimPeriostin is a protein from the Fascilin family. It is commonly present in normal tissues and is responsible for cell adhesion. Evidence has emerged showing that changes in periostin expression play an important role in tumor initiation, development, and progression. This study aims to investigate the effect of periostin in gastric cancer (GC) patients who underwent gastrectomy. Seven hundred and forty-seven GC patients who underwent gastrectomy between December 2006 and July 2011 were included in this study.MethodsSeven hundred and forty-seven cancer tissues and 70 paired adjacent normal tissues were collected. Periostin expression was evaluated by immunohistochemistry. The Gene Expression Omnibus database was used to study the association between the mRNA level and patient’s overall survival. The tumor microenvironment was also studied.ResultsPeriostin expression in stroma was downregulated in tumor tissues but it was upregulated in the epithelial cells. After dividing the tissues according to the Lauren Classification, we found that periostin expression in stroma and epithelial cells was higher in intestinal type than in diffuse type (P<0.001 and P=0.010, respectively). Periostin was an independent predictor of lymph node (LN) metastasis in GC patients. The study of CD163(+) tumor-associated macrophages (TAMs) revealed that in diffuse type GC, periostin expression was associated with CD163(+) TAMs.ConclusionWe found that the periostin expression can predict LN metastasis in patients undergoing curative gastrectomy. Intestinal type GC patients with high periostin level had both a favorable survival and lesser LN metastasis.

Stromal Protein-Mediated Immune Regulation in Digestive Cancers

“…In a colitis-associated colorectal cancer model using periostin-/- mice, lack of periostin expression resulted in fewer inflammatory peritoneal macrophages, as evidenced by a reduced expression of TNF-α and IL-1β, and up-regulation of TGF-β and IL-10 [ 155 ]. Interestingly, in patients with intestinal type gastric cancer, low periostin levels were associated with poor survival and lymph node metastasis [ 156 ]. The same study revealed that high periostin levels in diffuse gastric cancer was associated with high M2 macrophage infiltration, whereas this was not the case in intestinal gastric cancer.…”

Opposing biological functions of the cytoplasm and nucleus DAXX modified by SUMO-2/3 in gastric cancer

“…DAXX expression was detected in tumor tissue and normal tissue using IHC. Tissue microarrays (TMAs) were constructed and IHC was performed on the TMA slides as described previously 22 . Briefly, the slides were incubated with mouse anti-DAXX antibodies (1:200 dilution, Santa Cruz Biotechnology, Santa Cruz, Calif., USA; sc-8043), with antibody diluent buffer used as a negative control.…”

The Head and Neck Squamous Cell Carcinoma Microenvironment as a Potential Target for Cancer Therapy

“…However, periostin and tenascin-C should be mentioned here, as they represent the hot topic of ECM research in cancer biology. Nevertheless, the interpretation of the findings is frequently problematic and cancer type-specific [44,45,46,47]. Particular attention is dedicated to endogenous lectins such as galectins and their relation to ECM and cancer progression [48,49].…”