Cross-talk between endocytic clearance and secretion in macrophages

Kzhyshkowska, Julia; Krusell, Liis

doi:10.1016/j.imbio.2009.03.007

Cited by 28 publications

(20 citation statements)

References 121 publications

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“…LDL-R and LDLR-related protein (LRP, CD91) can endocytose lipoproteins through apoE or apoB interactions (Mahley and Rall 2000;van den Elzen et al 2005). Scavenger receptors bind and internalize a variety of ligands including lipoproteins and provide signals that can modulate the inflammatory and immune responses (Areschoug and Gordon 2009;Kzhyshkowska and Krusell 2009). Among the scavenger receptors expressed by DCs, non-modified LDL and HDL can bind to class B scavenger Fig.…”

Section: Discussionmentioning

confidence: 99%

High-density lipoprotein phospholipids interfere with dendritic cell Th1 functional maturation

et al. 2012

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Lipoproteins are both lipid carriers in the blood and regulators of essential biological processes. Several studies demonstrated that lipoproteins modified during pathological conditions could alter dendritic cell (DC) maturation. Here the immune function of non-pathological lipoproteins is addressed by analysing their impact on human DC maturation triggered by TLR ligands. Upon TLR4 stimulation, low- and high-density lipoproteins (LDL and HDL) strongly inhibited the ability of DC to induce a Th1 response of T cells, characterized by high levels of IFNγ secretion, whereas the effect of very low-density lipoprotein was subject to variations. HDL also inhibited the Th1 function of DC stimulated by TLR1/2 and TLR2/6 ligands. The phospholipid fraction from HDL retained the inhibitory activity of the lipoprotein. We identified the 1-palmitoyl-2-linoleyl-phosphatidylcholine (PLPC) as one active phospholipid that inhibited the Th1 function of mature DCs whereas the dipalmitoyl-phosphatidylcholine had no significant effect. The treatment of DC by PLPC, 24h before TLR4 stimulation, resulted in reduced activation of NF-κB. This study shows that some HDL phospholipids have a direct immunoregulatory function, by modulating DC ability to activate a Th1 response of T cells.

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Section: Discussionmentioning

confidence: 99%

High-density lipoprotein phospholipids interfere with dendritic cell Th1 functional maturation

et al. 2012

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“…Many of the expected AA markers could also be regulated by M-CSF, while a proportion of the genes were uniquely regulated by IL-4. Cell Biology and Membrane Dynamics in AAM Apart from their effects on specific receptors and antigen markers, IL-4 and IL-13 profoundly alter membrane flow in macrophages (Kzhyshkowska and Krusell, 2009;Montaner et al, 1999;. Human blood monocytederived macrophages displayed enhanced flow from the plasma membrane, through early endosomes into an expanded vacuolar compartment, accompanied by enhanced fusion with lysosomes (Montaner et al, 1999).…”

Section: Analytic Approachesmentioning

confidence: 99%

Alternative Activation of Macrophages: Mechanism and Functions

2010

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The concept of an alternative pathway of macrophage activation has stimulated interest in its definition, mechanism, and functional significance in homeostasis and disease. We assess recent research in this field, argue for a restricted definition, and explore pathways by which the T helper 2 (Th2) cell cytokines interleukin-4 (IL-4) and IL-13 mediate their effects on macrophage cell biology, their biosynthesis, and responses to a normal and pathological microenvironment. The stage is now set to gain deeper insights into the role of alternatively activated macrophages in immunobiology.

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“…Further, macrophages are able to sense the time point when an injury is terminated, to start the process of resolution of the inflammation, and to control the healing phase. We and others have demonstrated that macrophages orchestrate the healing phase by releasing tolerogenic cytokines and growth factors as well as extracellular matrix enzymes and structural components, and by clearance of waste molecules and apoptotic cells [31,33,38]. During the healing phase, macrophages suppress inflammatory activities of other immune cells, clear the tissue from apoptotic bodies and other unwanted-self components, induce and support angiogenesis to supply healing tissue with oxygen and nutrition, and stimulate somatic cells to reconstitute normal tissue composition [39].…”

Section: Diversity Of Monocyte-derived Macrophagesmentioning

confidence: 99%

“…As key inflammatory cytokines we took TNF-α (marker of M1 response) and CCL18 (marker of M2 response) which are shown to take part in chronic inflammation [102,103]. For the study of surface markers, we analyzed the expression of CD206 (marker of M2 response, strongly up-regulated in macrophages during chronic inflammation [104]) and stabilin-1 (marker of M2 response with tolerogenic functions [38]). …”

Section: Application Of Ex Vivo Monocyte-based Test Systemmentioning

confidence: 99%

Perspectives for Monocyte/Macrophage-Based Diagnostics of Chronic Inflammation

Kzhyshkowska¹,

Gudima

Moganti

et al. 2016

Transfus Med Hemother

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Low-grade chronic inflammation underlies the development of the most dangerous cardiometabolic disorders including type 2 diabetes and its vascular complications. In contrast to acute inflammation induced by bacteria and viruses, chronic inflammation can be driven by abnormal reaction to endogenous factors, including Th2 cytokines, metabolic factors like advanced glycation end products (AGEs), modified lipoproteins, or hyperglycemia. The key innate immune cells that recognize these factors in blood circulation are monocytes. Inflammatory programming of monocytes which migrate into tissues can, in turn, result into generation of tissue macrophages with pathological functions. Therefore, determination of the molecular and functional phenotype of circulating monocytes is a very promising diagnostic tool for the identification of hidden inflammation, which can precede the development of the pathology. Here we propose a new test system for the identification of inflammatory programming of monocytes: surface biomarkers and ex vivo functional system. We summarize the current knowledge about surface biomarkers for monocyte subsets, including CD16, CCR2, CX3CR1, CD64, stabilin-1 and CD36, and their association with inflammatory human disorders. Furthermore, we present the design of an ex vivo monocyte-based test system with minimal set of parameters as a potential diagnostic tool for the identification of personalized inflammatory responses.

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Cross-talk between endocytic clearance and secretion in macrophages

Cited by 28 publications

References 121 publications

High-density lipoprotein phospholipids interfere with dendritic cell Th1 functional maturation

High-density lipoprotein phospholipids interfere with dendritic cell Th1 functional maturation

Alternative Activation of Macrophages: Mechanism and Functions

Perspectives for Monocyte/Macrophage-Based Diagnostics of Chronic Inflammation

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